scholarly journals A Golgi stain for dendritic function?

10.1038/77650 ◽  
2000 ◽  
Vol 3 (8) ◽  
pp. 753-753
Author(s):  
Kevan Martin
Keyword(s):  
Golgi Stain

scholarly journals In Vivo Transduction of Central Neurons Using Recombinant Sindbis Virus

2001 ◽  
Vol 49 (12) ◽  
pp. 1497-1507 ◽  
Author(s):  
Takahiro Furuta ◽  
Ryohei Tomioka ◽  
Kousuke Taki ◽  
Kouichi Nakamura ◽  
Nobuaki Tamamaki ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Sindbis Virus ◽  
Fluorescent Protein ◽  
Immunocytochemical Staining ◽  
Thalamic Nuclei ◽  
Golgi Stain ◽  
Thalamocortical Axons ◽  
The Central Nervous System ◽  
Green Fluorescent

A new recombinant virus which labeled the infected neurons in a Golgi stainlike fashion was developed. The virus was based on a replication-defective Sindbis virus and was designed to express green fluorescent protein with a palmitoylation signal (pal-GFP). When the virus was injected into the ventrobasal thalamic nuclei, many neurons were visualized with the fluorescence of palGFP in the injection site. The labeling was enhanced by immunocytochemical staining with an antibody to green fluorescent protein to show the entire configuration of the dendrites. Thalamocortical axons of the infected neurons were also intensely immunostained in the somatosensory cortex. In contrast to palGFP, when DsRed with the same palmitoylation signal (palDsRed) was introduced into neurons with the Sindbis virus, palDsRed neither visualized the infected neurons in a Golgi stain-like manner nor stained projecting axons in the cerebral cortex. The palDsRed appeared to be aggregated or accumulated in some organelles in the infected neurons. Anterograde labeling with palGFP Sindbis virus was very intense, not only in thalamocortical neurons but also in callosal, striatonigral, and nigrostriatal neurons. Occasionally there were retrogradely labeled neurons that showed Golgi stain-like images. These results indicate that palGFP Sindbis virus can be used as an excellent anterograde tracer in the central nervous system.

Golgi Stain

1998 ◽  
Vol 21 (2) ◽  
pp. 165-166
Author(s):  
Venita Jay
Keyword(s):  
Golgi Stain

Neurons in Golgi-stain-like images revealed by GFP-adenovirus infection in vivo

2000 ◽  
Vol 38 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Nobuaki Tamamaki ◽  
Kouichi Nakamura ◽  
Takahiro Furuta ◽  
Ken Asamoto ◽  
Takeshi Kaneko
Keyword(s):  
Adenovirus Infection ◽  
Golgi Stain

Golgi stain identifies three types of fibres in fish muscle

1987 ◽  
Vol 8 (5) ◽  
pp. 418-427 ◽  
Author(s):  
Clara Franzini-Armstrong ◽  
William F. Gilly ◽  
Eva Aladjem ◽  
Denah Appelt
Keyword(s):  
Fish Muscle ◽  
Golgi Stain

Innervation of the Cochlea of the Guinea Pig by Use of the Golgi Stain

1975 ◽  
Vol 84 (4) ◽  
pp. 443-458 ◽  
Author(s):  
Catherine A. Smith
Keyword(s):  
Guinea Pig ◽  
Hair Cells ◽  
Guinea Pigs ◽  
Basilar Membrane ◽  
Organ Of Corti ◽  
Nerve Fibers ◽  
Outer Hair Cells ◽  
Single Row ◽  
Golgi Stain ◽  
Branching Patterns

Nerve fibers with distinctive branching patterns have been demonstrated in guinea pigs by use of the Golgi stain. The cochlear nerve fibers in the basal turn tend to supply a limited segment of the basilar membrane and have most endings on a single row of hair cells. The efferent olivocochlear nerve fibers ramify in a manner which varies from base to apex. Some efferents which terminate on outer hair cells also give branches which course in the inner spiral bundle. Other nerve fibers were studied in the spiral lamina which did not penetrate into the organ of Corti.

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