The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants

10.1038/70781 ◽  
1999 ◽  
Vol 17 (12) ◽  
pp. 1223-1226 ◽  
Author(s):  
Rafael Gutierrez-Campos ◽  
Juan Antonio Torres-Acosta ◽  
Luis Jorge Saucedo-Arias ◽  
Miguel Angel Gomez-Lim
Keyword(s):  
Transgenic Tobacco ◽  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Cysteine Proteinase Inhibitors

Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants

1993 ◽  
Vol 21 (4) ◽  
pp. 655-663 ◽  
Author(s):  
Sameer A. Masoud ◽  
Lowell B. Johnson ◽  
Frank F. White ◽  
Gerald R. Reeck
Keyword(s):  
Transgenic Tobacco ◽  
Proteinase Inhibitor ◽  
Cysteine Proteinase ◽  
Cysteine Proteinase Inhibitor ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

Molecular Characterization and Mapping of Murine Genes Encoding Three Members of the Stefin Family of Cysteine Proteinase Inhibitors

Genomics ◽  
1993 ◽  
Vol 15 (3) ◽  
pp. 507-514 ◽  
Author(s):  
Florence W.L. Tsui ◽  
Hing-Wo Tsui ◽  
Samuel Mok ◽  
Irena Mlinaric ◽  
Neal G. Copeland ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Cysteine Proteinase Inhibitors ◽  
Genes Encoding

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

Sign in / Sign up

Export Citation Format

Share Document