Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Nature ◽  
10.1038/35888 ◽  
1998 ◽  
Vol 391 (6669) ◽  
pp. 806-811 ◽  
Author(s):  
Andrew Fire ◽  
SiQun Xu ◽  
Mary K. Montgomery ◽  
Steven A. Kostas ◽  
Samuel E. Driver ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Double Stranded Rna ◽  
Genetic Interference

Uptake and Bioactivity of Chitosan/Double-Stranded RNA Polyplex Nanoparticles in Caenorhabditis elegans

2019 ◽  
Vol 53 (7) ◽  
pp. 3832-3840 ◽  
Author(s):  
Stuart S. Lichtenberg ◽  
Olga V. Tsyusko ◽  
Subba R. Palli ◽  
Jason M. Unrine
Keyword(s):  
Caenorhabditis Elegans ◽  
Double Stranded Rna

scholarly journals New strains for tissue-specific RNAi studies in Caenorhabditis elegans

2018 ◽  
Author(s):  
Jason S. Watts ◽  
Henry F. Harrison ◽  
Shizue Omi ◽  
Quentin Guenthers ◽  
James Dalelio ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Caenorhabditis Elegans ◽  
Gene Function ◽  
Target Genes ◽  
Resistant Mutant ◽  
Knockout Mutant ◽  
Tissue Specific ◽  
Double Stranded Rna ◽  
C Elegans ◽  
Insight Into

AbstractRNA interference is a powerful tool for dissecting gene function. In Caenorhabditis elegans, ingestion of double stranded RNA causes strong, systemic knockdown of target genes. Further insight into gene function can be revealed by tissue-specific RNAi techniques. Currently available tissue-specific C. elegans strains rely on rescue of RNAi function in a desired tissue or cell in an otherwise RNAi deficient genetic background. We attempted to assess the contribution of specific tissues to polyunsaturated fatty acid (PUFA) synthesis using currently available tissue-specific RNAi strains. We discovered that rde-1 (ne219), a commonly used RNAi-resistant mutant strain, retains considerable RNAi capacity against RNAi directed at PUFA synthesis genes. By measuring changes in the fatty acid products of the desaturase enzymes that synthesize PUFAs, we found that the before mentioned strain, rde-1 (ne219) and the reported germline only RNAi strain, rrf-1 (pk1417) are not appropriate genetic backgrounds for tissue-specific RNAi experiments. However, the knockout mutant rde-1 (ne300) was strongly resistant to dsRNA induced RNAi, and thus is more appropriate for construction of a robust tissue-specific RNAi strains. Using newly constructed strains in the rde-1(null) background, we found considerable desaturase activity in intestinal, epidermal, and germline tissues, but not in muscle. The RNAi-specific strains reported in this study will be useful tools for C. elegans researchers studying a variety of biological processes.

Comparison of Nanomaterials for Delivery of Double-Stranded RNA in Caenorhabditis elegans

2020 ◽  
Vol 68 (30) ◽  
pp. 7926-7934 ◽  
Author(s):  
Stuart S. Lichtenberg ◽  
Jerome Laisney ◽  
Zeinah Elhaj Baddar ◽  
Olga V. Tsyusko ◽  
Subba R. Palli ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Double Stranded Rna

scholarly journals RNAi targeting Caenorhabditis elegans α-arrestins has little effect on lifespan

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1515
Author(s):  
Sangsoon Park ◽  
Yoonji Jung ◽  
Seon Woo A. An ◽  
Heehwa G. Son ◽  
Wooseon Hwang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Functional Role ◽  
Future Research ◽  
Biological Processes ◽  
Receptor Desensitization ◽  
Double Stranded Rna ◽  
C Elegans

Background: α-arrestins are a family of proteins that are implicated in multiple biological processes, including metabolism and receptor desensitization. Methods: Here, we sought to examine the roles of α-arrestins in the longevity of Caenorhabditis elegans through an RNA interference screen. Results: We found that feeding worms with bacteria expressing double-stranded RNA against each of 24 out of total 29 C. elegans α-arrestins had little effect on lifespan. Thus, individual C. elegans α-arrestins may have minor effects on longevity. Conclusions: This study will provide useful information for future research on the functional role of α-arrestins in aging and longevity.

scholarly journals Transgene-mediated cosuppression in the C. elegans germ line

2000 ◽  
Vol 14 (13) ◽  
pp. 1578-1583 ◽  
Author(s):  
Abby F. Dernburg ◽  
Jonathan Zalevsky ◽  
Mónica P. Colaiácovo ◽  
Anne M. Villeneuve
Keyword(s):  
Caenorhabditis Elegans ◽  
Germ Line ◽  
Target Specificity ◽  
Double Stranded Rna ◽  
C Elegans ◽  
Physical Association ◽  
Endogenous Genes ◽  
Chromosomal Loci

Functional silencing of chromosomal loci can be induced by transgenes (cosuppression) or by introduction of double-stranded RNA (RNAi). Here, we demonstrate the generality of and define rules for a transgene-mediated cosuppression phenomenon in the Caenorhabditis elegans germ line. Functional repression is not a consequence of persistent physical association between transgenes and endogenous genes or of mutations in affected genes. The cosuppression mechanism likely involves an RNA mediator that defines its target specificity, reminiscent of RNAi. Cosuppression is strongly abrogated inrde-2 and mut-7 mutants, but is not blocked in anrde-1 mutant, indicating that cosuppression and RNAi have overlapping but distinct genetic requirements.

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