Uptake and Bioactivity of Chitosan/Double-Stranded RNA Polyplex Nanoparticles in Caenorhabditis elegans

2019 ◽  
Vol 53 (7) ◽  
pp. 3832-3840 ◽  
Author(s):  
Stuart S. Lichtenberg ◽  
Olga V. Tsyusko ◽  
Subba R. Palli ◽  
Jason M. Unrine
Keyword(s):  
Caenorhabditis Elegans ◽  
Double Stranded Rna

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Nature ◽  
10.1038/35888 ◽  
1998 ◽  
Vol 391 (6669) ◽  
pp. 806-811 ◽  
Author(s):  
Andrew Fire ◽  
SiQun Xu ◽  
Mary K. Montgomery ◽  
Steven A. Kostas ◽  
Samuel E. Driver ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Double Stranded Rna ◽  
Genetic Interference

scholarly journals New strains for tissue-specific RNAi studies in Caenorhabditis elegans

2018 ◽  
Author(s):  
Jason S. Watts ◽  
Henry F. Harrison ◽  
Shizue Omi ◽  
Quentin Guenthers ◽  
James Dalelio ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Caenorhabditis Elegans ◽  
Gene Function ◽  
Target Genes ◽  
Resistant Mutant ◽  
Knockout Mutant ◽  
Tissue Specific ◽  
Double Stranded Rna ◽  
C Elegans ◽  
Insight Into

AbstractRNA interference is a powerful tool for dissecting gene function. In Caenorhabditis elegans, ingestion of double stranded RNA causes strong, systemic knockdown of target genes. Further insight into gene function can be revealed by tissue-specific RNAi techniques. Currently available tissue-specific C. elegans strains rely on rescue of RNAi function in a desired tissue or cell in an otherwise RNAi deficient genetic background. We attempted to assess the contribution of specific tissues to polyunsaturated fatty acid (PUFA) synthesis using currently available tissue-specific RNAi strains. We discovered that rde-1 (ne219), a commonly used RNAi-resistant mutant strain, retains considerable RNAi capacity against RNAi directed at PUFA synthesis genes. By measuring changes in the fatty acid products of the desaturase enzymes that synthesize PUFAs, we found that the before mentioned strain, rde-1 (ne219) and the reported germline only RNAi strain, rrf-1 (pk1417) are not appropriate genetic backgrounds for tissue-specific RNAi experiments. However, the knockout mutant rde-1 (ne300) was strongly resistant to dsRNA induced RNAi, and thus is more appropriate for construction of a robust tissue-specific RNAi strains. Using newly constructed strains in the rde-1(null) background, we found considerable desaturase activity in intestinal, epidermal, and germline tissues, but not in muscle. The RNAi-specific strains reported in this study will be useful tools for C. elegans researchers studying a variety of biological processes.

Comparison of Nanomaterials for Delivery of Double-Stranded RNA in Caenorhabditis elegans

2020 ◽  
Vol 68 (30) ◽  
pp. 7926-7934 ◽  
Author(s):  
Stuart S. Lichtenberg ◽  
Jerome Laisney ◽  
Zeinah Elhaj Baddar ◽  
Olga V. Tsyusko ◽  
Subba R. Palli ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Double Stranded Rna

scholarly journals RNAi targeting Caenorhabditis elegans α-arrestins has little effect on lifespan

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1515
Author(s):  
Sangsoon Park ◽  
Yoonji Jung ◽  
Seon Woo A. An ◽  
Heehwa G. Son ◽  
Wooseon Hwang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Functional Role ◽  
Future Research ◽  
Biological Processes ◽  
Receptor Desensitization ◽  
Double Stranded Rna ◽  
C Elegans

Background: α-arrestins are a family of proteins that are implicated in multiple biological processes, including metabolism and receptor desensitization. Methods: Here, we sought to examine the roles of α-arrestins in the longevity of Caenorhabditis elegans through an RNA interference screen. Results: We found that feeding worms with bacteria expressing double-stranded RNA against each of 24 out of total 29 C. elegans α-arrestins had little effect on lifespan. Thus, individual C. elegans α-arrestins may have minor effects on longevity. Conclusions: This study will provide useful information for future research on the functional role of α-arrestins in aging and longevity.

scholarly journals Transgene-mediated cosuppression in the C. elegans germ line

2000 ◽  
Vol 14 (13) ◽  
pp. 1578-1583 ◽  
Author(s):  
Abby F. Dernburg ◽  
Jonathan Zalevsky ◽  
Mónica P. Colaiácovo ◽  
Anne M. Villeneuve
Keyword(s):  
Caenorhabditis Elegans ◽  
Germ Line ◽  
Target Specificity ◽  
Double Stranded Rna ◽  
C Elegans ◽  
Physical Association ◽  
Endogenous Genes ◽  
Chromosomal Loci

Functional silencing of chromosomal loci can be induced by transgenes (cosuppression) or by introduction of double-stranded RNA (RNAi). Here, we demonstrate the generality of and define rules for a transgene-mediated cosuppression phenomenon in the Caenorhabditis elegans germ line. Functional repression is not a consequence of persistent physical association between transgenes and endogenous genes or of mutations in affected genes. The cosuppression mechanism likely involves an RNA mediator that defines its target specificity, reminiscent of RNAi. Cosuppression is strongly abrogated inrde-2 and mut-7 mutants, but is not blocked in anrde-1 mutant, indicating that cosuppression and RNAi have overlapping but distinct genetic requirements.

scholarly journals Heat Shock in C. elegans Induces Downstream of Gene Transcription andAccumulation of Double-Stranded RNA

2018 ◽  
Author(s):  
Marko Melnick ◽  
Patrick Gonzales ◽  
Joseph Cabral ◽  
Mary A. Allen ◽  
Robin D. Dowell ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Caenorhabditis Elegans ◽  
Heat Shock ◽  
Gene Transcription ◽  
Specific Monoclonal Antibody ◽  
Rna Seq ◽  
Antisense Transcripts ◽  
Double Stranded Rna ◽  
C Elegans

AbstractWe observed that heat shock of Caenorhabditis elegans leads to the formation of nuclear double-stranded RNA (dsRNA) foci, detectable with a dsRNA-specific monoclonal antibody. These foci significantly overlap with nuclear HSF-1 granules. To investigate the molecular mechanism(s) underlying dsRNA foci formation, we used RNA-seq to globally characterize total RNA and immunoprecipitated dsRNA from control and heat shocked worms. We find antisense transcripts are generally increased after heat shock, and a subset of both sense and antisense transcripts enriched in the dsRNA pool by heat shock overlap with dsRNA transcripts enriched by deletion of tdp-1, which encodes the C. elegans ortholog of TDP-43. Interestingly, transcripts involved in translation are over-represented in the dsRNAs induced by either heat shock or deletion of tdp-1. Also enriched in the dsRNA transcripts are sequences downstream of annotated genes (DoGs), which we globally quantified with a new algorithm. To validate these observations, we used fluorescence in situ hybridization (FISH) to confirm both antisense and downstream of gene transcription for eif-3.B, one of the affected loci we identified.

scholarly journals Transmission Dynamics of Heritable Silencing Induced by Double-Stranded RNA in Caenorhabditis elegans

Genetics ◽  
2008 ◽  
Vol 180 (3) ◽  
pp. 1275-1288 ◽  
Author(s):  
Rosa M. Alcazar ◽  
Rueyling Lin ◽  
Andrew Z. Fire
Keyword(s):  
Caenorhabditis Elegans ◽  
Transmission Dynamics ◽  
Double Stranded Rna

scholarly journals A Family of K+ Channel Ancillary Subunits Regulate Taste Sensitivity in Caenorhabditis elegans

2005 ◽  
Vol 280 (23) ◽  
pp. 21893-21899 ◽  
Author(s):  
Ki Ho Park ◽  
Leonardo Hernandez ◽  
Shi-Qing Cai ◽  
Yi Wang ◽  
Federico Sesti
Keyword(s):  
Caenorhabditis Elegans ◽  
Mammalian Cells ◽  
K Channel ◽  
Sensory Thresholds ◽  
Test Plate ◽  
Double Stranded Rna ◽  
Voltage Gated ◽  
C Elegans ◽  
Chemosensory Neuron

We have identified a family of ancillary subunits of K+ channels in Caenorhabditis elegans. MPS-1 and its related members MPS-2, MPS-3, and MPS-4 are detected in the nervous system of the nematode. Electrophysiological analysis in ASE neurons and mammalian cells and epigenetic inactivation by double-stranded RNA interference (RNAi) in vivo show that each MPS can associate with and functionally endow the voltage-gated K+ channel KVS-1. In the chemosensory neuron ADF, three different MPS subunits combine with KVS-1 to form both binary (MPS-1·KVS-1) and ternary (MPS-2·MPS-3·KVS-1) complexes. RNAi of mps-2, mps-3, or both, enhance the taste of the animal for sodium without altering the susceptibility to other attractants. When sodium is introduced in the test plate as background or as antagonist attractant, the nematode loses the ability to recognize a second attractant. Thus, it appears that the chemosensory apparatus of C. elegans uses sensory thresholds and that a voltage-gated K+ channel is specifically required for this mechanism.

scholarly journals RNA Interference Can Target Pre-mRNA: Consequences for Gene Expression in a Caenorhabditis elegans Operon

Genetics ◽  
1999 ◽  
Vol 153 (3) ◽  
pp. 1245-1256
Author(s):  
Julia M Bosher ◽  
Pascale Dufourcq ◽  
Satis Sookhareea ◽  
Michel Labouesse
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Gene Function ◽  
Direct Evidence ◽  
Embryonic Lethality ◽  
Null Mutation ◽  
Null Mutant ◽  
Double Stranded Rna ◽  
Severe Loss

Abstract In nematodes, flies, trypanosomes, and planarians, introduction of double-stranded RNA results in sequence-specific inactivation of gene function, a process termed RNA interference (RNAi). We demonstrate that RNAi against the Caenorhabditis elegans gene lir-1, which is part of the lir-1/lin-26 operon, induced phenotypes very different from a newly isolated lir-1 null mutation. Specifically, lir-1(RNAi) induced embryonic lethality reminiscent of moderately strong lin-26 alleles, whereas the lir-1 null mutant was viable. We show that the lir-1(RNAi) phenotypes resulted from a severe loss of lin-26 gene expression. In addition, we found that RNAi directed against lir-1 or lin-26 introns induced similar phenotypes, so we conclude that lir-1(RNAi) targets the lir-1/lin-26 pre-mRNA. This provides direct evidence that RNA interference can prevent gene expression by targeting nuclear transcripts. Our results highlight that caution may be necessary when interpreting RNA interference without the benefit of mutant alleles.

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