scholarly journals Correction: Muscle-specific gene expression controlled by a regulatory element lacking a MyoD1-binding site

Nature ◽  
1990 ◽  
Vol 345 (6273) ◽  
pp. 364-364 ◽  
Author(s):  
Timothy J. Baldwin ◽  
Steven J. Burden
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Regulatory Element ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals A new function for methyl CpG: Creating a C/EBPα binding site & mediating tissue‐specific gene expression

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Raghunath Chatterjee ◽  
Vikas Rishi ◽  
Julian Rozenberg ◽  
Paramita Bhattacharya ◽  
Kimberly Glass ◽  
...  
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Specific Gene ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Specific Gene Expression

scholarly journals A small regulatory element from chromosome 19 enhances liver-specific gene expression

Gene Therapy ◽  
2008 ◽  
Vol 16 (1) ◽  
pp. 43-51 ◽  
Author(s):  
C Li ◽  
M Hirsch ◽  
P Carter ◽  
A Asokan ◽  
X Zhou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Element ◽  
Specific Gene ◽  
Chromosome 19 ◽  
Specific Gene Expression

scholarly journals The Role of Forkhead Box A2 to Restrict Androgen-Regulated Gene Expression of Lipocalin 5 in the Mouse Epididymis

2006 ◽  
Vol 20 (10) ◽  
pp. 2418-2431 ◽  
Author(s):  
Xiuping Yu ◽  
Kichiya Suzuki ◽  
Yongqing Wang ◽  
Aparna Gupta ◽  
Renjie Jin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Binding Site ◽  
Binding Sites ◽  
Transient Transfection ◽  
Specific Gene ◽  
Promoter Fragment ◽  
Specific Gene Expression ◽  
Principal Cells ◽  
Forkhead Box

Abstract Murine epididymal retinoic acid-binding protein [or lipocalin 5 (Lcn5)] is synthesized and secreted by the principal cells of the mouse middle/distal caput epididymidis. A 5-kb promoter fragment of the Lcn5 gene can dictate androgen-dependent and epididymis region-specific gene expression in transgenic mice. Here, we reported that the 1.8-kb Lcn5 promoter confers epididymis region-specific gene expression in transgenic mice. To decipher the mechanism that directs transcription, 14 chimeric constructs that sequentially removed 100 bp of 1.8-kb Lcn5 promoter were generated and transfected into epididymal cells and nonepididymal cells. Transient transfection analysis revealed that 1.3 kb promoter fragment gave the strongest response to androgens. Between the 1.2-kb to 1.3-kb region, two androgen receptor (AR) binding sites were identified. Adjacent to AR binding sites, a Foxa2 [Fox (Forkhead box) subclass A] binding site was confirmed by gel shift assay. Similar Foxa binding sites were also found on the promoters of human and rat Lcn5, indicating the Foxa binding site is conserved among species. We previously reported that among the three members of Foxa family, Foxa1 and Foxa3 were absent in the epididymis whereas Foxa2 was detected in epididymal principal cells. Here, we report that Foxa2 displays a region-specific expression pattern along the epididymis: no staining observed in initial segment, light staining in proximal caput, gradiently heavier staining in middle and distal caput, and strongest staining in corpus and cauda, regions with little or no expression of Lcn5. In transient transfection experiments, Foxa2 expression inhibits AR induction of the Lcn5 promoter, which is consistent with the lack of expression of Lcn5 in the corpus and cauda. We conclude that Foxa2 functions as a repressor that restricts AR regulation of Lcn5 to a segment-specific pattern in the epididymis.

The proximal promoter of the mouse arrestin gene directs gene expression in photoreceptor cells and contains an evolutionarily conserved retinal factor-binding site

1993 ◽  
Vol 13 (7) ◽  
pp. 4400-4408
Author(s):  
T Kikuchi ◽  
K Raju ◽  
M L Breitman ◽  
T Shinohara
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Critical Role ◽  
Proximal Promoter ◽  
Specific Gene ◽  
Regulatory Sequences ◽  
Related Sequence ◽  
Specific Gene Expression ◽  
Nuclear Factors ◽  
Evolutionarily Conserved

Regulatory sequences and nuclear factors governing tissue-restricted expression of the mouse arrestin gene were investigated. The results showed that while proximal promoter sequence positions -38 to +304 are sufficient to direct low levels of retina-specific gene expression, sequences extending upstream to position -209 support higher levels of expression in the retina, as well as detectable expression in the lens, pineal gland, and brain. Within the interval between positions -209 and -38, a broadly expressed nuclear factor, Bd, binds to sequences centered between positions -205 and -185, a region which contains two direct repeats of the hexamer, TGACCT. The proximal promoter binds three apparently retina-specific nuclear factors, Bp1, Bp2, and Bp3, through overlapping sequences centered between positions -25 and -15. Bp1 and Bp3 also recognize a closely related sequence found in the promoter regions of several other vertebrate photoreceptor-specific genes. Moreover, the consensus binding site for Bp1, designated PCE I, is identical to RCS I, an element known to play a critical role eliciting photoreceptor-specific gene expression in Drosophila melanogaster. The results suggest that PCE I and RCS I are functionally as well as structurally similar and that, despite marked differences in the fly and vertebrate visual systems, the transcriptional machinery involved in photoreceptor-specific gene expression has been strongly evolutionarily conserved.

scholarly journals The proximal promoter of the mouse arrestin gene directs gene expression in photoreceptor cells and contains an evolutionarily conserved retinal factor-binding site.

1993 ◽  
Vol 13 (7) ◽  
pp. 4400-4408 ◽  
Author(s):  
T Kikuchi ◽  
K Raju ◽  
M L Breitman ◽  
T Shinohara
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Critical Role ◽  
Proximal Promoter ◽  
Specific Gene ◽  
Regulatory Sequences ◽  
Related Sequence ◽  
Specific Gene Expression ◽  
Nuclear Factors ◽  
Evolutionarily Conserved

Regulatory sequences and nuclear factors governing tissue-restricted expression of the mouse arrestin gene were investigated. The results showed that while proximal promoter sequence positions -38 to +304 are sufficient to direct low levels of retina-specific gene expression, sequences extending upstream to position -209 support higher levels of expression in the retina, as well as detectable expression in the lens, pineal gland, and brain. Within the interval between positions -209 and -38, a broadly expressed nuclear factor, Bd, binds to sequences centered between positions -205 and -185, a region which contains two direct repeats of the hexamer, TGACCT. The proximal promoter binds three apparently retina-specific nuclear factors, Bp1, Bp2, and Bp3, through overlapping sequences centered between positions -25 and -15. Bp1 and Bp3 also recognize a closely related sequence found in the promoter regions of several other vertebrate photoreceptor-specific genes. Moreover, the consensus binding site for Bp1, designated PCE I, is identical to RCS I, an element known to play a critical role eliciting photoreceptor-specific gene expression in Drosophila melanogaster. The results suggest that PCE I and RCS I are functionally as well as structurally similar and that, despite marked differences in the fly and vertebrate visual systems, the transcriptional machinery involved in photoreceptor-specific gene expression has been strongly evolutionarily conserved.

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