The Caenorhabditis elegans heterochronic gene lin-14 encodes a nuclear protein that forms a temporal developmental switch

Nature ◽  
1989 ◽  
Vol 338 (6213) ◽  
pp. 313-319 ◽  
Author(s):  
Gary Ruvkun ◽  
John Giusto
Keyword(s):  
Caenorhabditis Elegans ◽  
Nuclear Protein ◽  
Heterochronic Gene ◽  
Developmental Switch

scholarly journals lin-8, Which Antagonizes Caenorhabditis elegans Ras-Mediated Vulval Induction, Encodes a Novel Nuclear Protein That Interacts With the LIN-35 Rb Protein

Genetics ◽  
2005 ◽  
Vol 171 (3) ◽  
pp. 1017-1031 ◽  
Author(s):  
Ewa M. Davison ◽  
Melissa M. Harrison ◽  
Albertha J. M. Walhout ◽  
Marc Vidal ◽  
H. Robert Horvitz
Keyword(s):  
Caenorhabditis Elegans ◽  
Nuclear Protein ◽  
Rb Protein

scholarly journals The nuclear receptor gene nhr-25 plays multiple roles in the Caenorhabditis elegans heterochronic gene network to control the larva-to-adult transition

2010 ◽  
Vol 344 (2) ◽  
pp. 1100-1109 ◽  
Author(s):  
Kazumasa Hada ◽  
Masako Asahina ◽  
Hiroshi Hasegawa ◽  
Yasunori Kanaho ◽  
Frank J. Slack ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Nuclear Receptor ◽  
Gene Network ◽  
Receptor Gene ◽  
Multiple Roles ◽  
Adult Transition ◽  
Heterochronic Gene

The terminal differentiation factor LIN-29 is required for proper vulval morphogenesis and egg laying in Caenorhabditis elegans

Development ◽  
1997 ◽  
Vol 124 (21) ◽  
pp. 4333-4342 ◽  
Author(s):  
J.C. Bettinger ◽  
S. Euling ◽  
A.E. Rougvie
Keyword(s):  
Caenorhabditis Elegans ◽  
Terminal Differentiation ◽  
Egg Laying ◽  
Small Subset ◽  
Wild Type ◽  
Vulval Development ◽  
Genetic Mosaics ◽  
Hypodermal Cell ◽  
Heterochronic Gene ◽  
Final Molt

Caenorhabditis elegans vulval development culminates during exit from the L4-to-adult molt with the formation of an opening through the adult hypodermis and cuticle that is used for egg laying and mating. Vulva formation requires the heterochronic gene lin-29, which triggers hypodermal cell terminal differentiation during the final molt. lin-29 mutants are unable to lay eggs or mate because no vulval opening forms; instead, a protrusion forms at the site of the vulva. We demonstrate through analysis of genetic mosaics that lin-29 is absolutely required in a small subset of lateral hypodermal seam cells, adjacent to the vulva, for wild-type vulva formation and egg laying. However, lin-29 function is not strictly limited to the lateral hypodermis. First, LIN-29 accumulates in many non-hypodermal cells with known roles in vulva formation or egg laying. Second, animals homozygous for one lin-29 allele, ga94, have the vulval defect and cannot lay eggs, despite having a terminally differentiated adult lateral hypodermis. Finally, vulval morphogenesis and egg laying requires lin-29 activity within the EMS lineage, a lineage that does not generate hypodermal cells.

Characterization of STIP, a multi-domain nuclear protein, highly conserved in metazoans, and essential for embryogenesis in Caenorhabditis elegans

2007 ◽  
Vol 313 (7) ◽  
pp. 1460-1472 ◽  
Author(s):  
Qiongmei Ji ◽  
Cheng-Han Huang ◽  
Jianbin Peng ◽  
Sarwar Hashmi ◽  
Tianzhang Ye ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Nuclear Protein

scholarly journals Isoform-Specific Mutations in the Caenorhabditis elegans Heterochronic Gene lin-14 Affect Stage-Specific Patterning

Genetics ◽  
2001 ◽  
Vol 157 (1) ◽  
pp. 199-209 ◽  
Author(s):  
Brenda J Reinhart ◽  
Gary Ruvkun
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Larval Stage ◽  
Protein Isoforms ◽  
Specific Cell ◽  
Temporal Expression ◽  
Critical Determinant ◽  
Temporal Gradient ◽  
Protein Levels ◽  
Heterochronic Gene

Abstract The Caenorhabditis elegans heterochronic gene lin-14 specifies the temporal sequence of postembryonic developmental events. lin-14, which encodes differentially spliced LIN-14A and LIN-14B1/B2 protein isoforms, acts at distinct times during the first larval stage to specify first and second larval stage-specific cell lineages. Proposed models for the molecular basis of these two lin-14 gene activities have included the production of functionally distinct isoforms and the generation of a temporal gradient of LIN-14 protein. We report here that loss of the LIN-14B1/B2 isoforms alone affects one of the two lin-14 temporal patterning functions, the specification of second larval stage lineages. A temporal expression difference between LIN-14A and LIN-14B1/B2 is not responsible for the stage-specific phenotype: protein levels of all LIN-14 isoforms are high in early first larval stage animals and decrease during the first larval stage. However, LIN-14A can partially substitute for LIN-14B1/B2 when expressed at a higher-than-normal level in the late L1 stage. These data indicate that LIN-14B1/B2 isoforms do not provide a distinct function of the lin-14 locus in developmental timing but rather may contribute to an overall level of LIN-14 protein that is the critical determinant of temporal cell fate.

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