cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines

Nature ◽  
1986 ◽  
Vol 320 (6064) ◽  
pp. 695-699 ◽  
Author(s):  
Christer Betsholtz ◽  
Ann Johnsson ◽  
Carl-Henrik Heldin ◽  
Bengt Westermark ◽  
Peter Lind ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Tumour Cell ◽  
Cdna Sequence ◽  
Chromosomal Localization ◽  
Human Platelet ◽  
Platelet Derived Growth Factor ◽  
Tumour Cell Lines ◽  
A Chain

scholarly journals Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

1988 ◽  
Vol 8 (2) ◽  
pp. 571-577 ◽  
Author(s):  
F Rorsman ◽  
M Bywater ◽  
T J Knott ◽  
J Scott ◽  
C Betsholtz
Keyword(s):  
Growth Factor ◽  
Genomic Dna ◽  
Human Platelet ◽  
Restriction Endonuclease Analysis ◽  
Platelet Derived Growth Factor ◽  
Alternative Exon ◽  
Chain Gene ◽  
Precursor Proteins ◽  
Exon Usage ◽  
A Chain

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

scholarly journals Production of transforming growth factor-α in human tumour cell lines

1989 ◽  
Vol 59 (5) ◽  
pp. 761-765 ◽  
Author(s):  
K Imanishi ◽  
K Yamaguchi ◽  
M Suzuki ◽  
S Honda ◽  
N Yanaihara ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Tumour Cell ◽  
Transforming Growth Factor ◽  
Human Tumour ◽  
Transforming Growth Factor Α ◽  
Tumour Cell Lines ◽  
Factor Α ◽  
Human Tumour Cell Lines

scholarly journals Promoter region of the human platelet-derived growth factor A-chain gene.

1991 ◽  
Vol 88 (5) ◽  
pp. 1686-1690 ◽  
Author(s):  
Y. Takimoto ◽  
Z. Y. Wang ◽  
K. Kobler ◽  
T. F. Deuel
Keyword(s):  
Growth Factor ◽  
Promoter Region ◽  
Human Platelet ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
A Chain

scholarly journals A dual immunocytochemical assay for oestrogen and epidermal growth factor receptors in tumour cell lines

1994 ◽  
Vol 26 (4) ◽  
pp. 306-310 ◽  
Author(s):  
A. K. Sharma ◽  
K. Horgan ◽  
R. A. McClelland ◽  
A. G. Douglas-Jones ◽  
T. Van Agthoven ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Lines ◽  
Tumour Cell ◽  
Growth Factor Receptors ◽  
Epidermal Growth Factor Receptors ◽  
Immunocytochemical Assay ◽  
Epidermal Growth ◽  
Tumour Cell Lines

Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins

1988 ◽  
Vol 8 (2) ◽  
pp. 571-577
Author(s):  
F Rorsman ◽  
M Bywater ◽  
T J Knott ◽  
J Scott ◽  
C Betsholtz
Keyword(s):  
Growth Factor ◽  
Genomic Dna ◽  
Human Platelet ◽  
Restriction Endonuclease Analysis ◽  
Platelet Derived Growth Factor ◽  
Alternative Exon ◽  
Chain Gene ◽  
Precursor Proteins ◽  
Exon Usage ◽  
A Chain

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

scholarly journals Distribution of mRNA for human epiregulin, a differentially expressed member of the epidermal growth factor family

1997 ◽  
Vol 326 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Hitoshi TOYODA ◽  
Toshi KOMURASAKI ◽  
Daisuke UCHIDA ◽  
Sigeo MORIMOTO
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Lines ◽  
Tumour Cell ◽  
Biologically Active ◽  
Soluble Form ◽  
Egf Receptors ◽  
Epidermal Growth ◽  
Tumour Cell Lines ◽  
Egf Family

We have recently identified epiregulin as a new growth regulator and a member of the epidermal growth factor (EGF) family. Epiregulin has certain characteristics that are different from those of the classical members of the EGF family, EGF and transforming growth factor α, including mitogenic responses on several normal cells and binding to EGF receptors on epidermoid carcinoma A431 cells. In the present study we cloned and identified the expression of human epiregulin transcript. The human epiregulin gene encoded a 163-residue putative transmembrane precursor containing an EGF-like domain in the internal segment, and the structural organization was similar to that of other members of the EGF family that bind to EGF receptors. Northern blot analysis showed the expression of human epiregulin to be mainly on peripheral blood macrophages and the placenta in normal tissues, and was highest on epithelial tumour cell lines in various types of tumour cell lines. The expression profile was quite different from that of other members of the EGF family in normal and tumour cells. Recombinant expression in mammalian cells also showed that human epiregulin was secreted as a soluble form of approx. 5 kDa that is biologically active on the basis of the stimulation of DNA synthesis. Our findings suggest that epiregulin is involved in certain physiological processes such as maintenance or development of normal cell growth, and the progression of carcinomas.

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