scholarly journals An S1 nuclease-sensitive region in the first intron of human platelet-derived growth factor A-chain gene contains a negatively acting cell type-specific regulatory element

1994 ◽  
Vol 22 (3) ◽  
pp. 457-464 ◽  
Author(s):  
Zhao-Yi Wang ◽  
Nobuyoshi Masaharu ◽  
Qing-Qing Qiu ◽  
Yasuo Takimato ◽  
Thomas F. Deuel
Keyword(s):  
Growth Factor ◽  
Human Platelet ◽  
Regulatory Element ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
Cell Type ◽  
S1 Nuclease ◽  
First Intron ◽  
Cell Type Specific ◽  
A Chain

scholarly journals Identification of a Cell Type-specific Enhancer in the Distal 5′-Region of the Platelet-derived Growth Factor A-chain Gene

1998 ◽  
Vol 273 (50) ◽  
pp. 33239-33246 ◽  
Author(s):  
Raymond S. Maul ◽  
Hongxing Zhang ◽  
James D. Reid ◽  
Nancy G. Pedigo ◽  
David M. Kaetzel
Keyword(s):  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
Cell Type ◽  
A Cell ◽  
Cell Type Specific ◽  
A Chain

scholarly journals Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

1988 ◽  
Vol 8 (2) ◽  
pp. 571-577 ◽  
Author(s):  
F Rorsman ◽  
M Bywater ◽  
T J Knott ◽  
J Scott ◽  
C Betsholtz
Keyword(s):  
Growth Factor ◽  
Genomic Dna ◽  
Human Platelet ◽  
Restriction Endonuclease Analysis ◽  
Platelet Derived Growth Factor ◽  
Alternative Exon ◽  
Chain Gene ◽  
Precursor Proteins ◽  
Exon Usage ◽  
A Chain

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

scholarly journals Promoter region of the human platelet-derived growth factor A-chain gene.

1991 ◽  
Vol 88 (5) ◽  
pp. 1686-1690 ◽  
Author(s):  
Y. Takimoto ◽  
Z. Y. Wang ◽  
K. Kobler ◽  
T. F. Deuel
Keyword(s):  
Growth Factor ◽  
Promoter Region ◽  
Human Platelet ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
A Chain

Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins

1988 ◽  
Vol 8 (2) ◽  
pp. 571-577
Author(s):  
F Rorsman ◽  
M Bywater ◽  
T J Knott ◽  
J Scott ◽  
C Betsholtz
Keyword(s):  
Growth Factor ◽  
Genomic Dna ◽  
Human Platelet ◽  
Restriction Endonuclease Analysis ◽  
Platelet Derived Growth Factor ◽  
Alternative Exon ◽  
Chain Gene ◽  
Precursor Proteins ◽  
Exon Usage ◽  
A Chain

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

scholarly journals Alternatively spliced platelet-derived growth factor A-chain transcripts are not tumor specific but encode normal cellular proteins.

1990 ◽  
Vol 10 (11) ◽  
pp. 6051-6054 ◽  
Author(s):  
R M Young ◽  
A E Mendoza ◽  
T Collins ◽  
S H Orkin
Keyword(s):  
Growth Factor ◽  
Immunohistochemical Localization ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
S1 Nuclease ◽  
Normal Tissues ◽  
Alternative Mrna Splicing ◽  
Alternatively Spliced ◽  
A Chain ◽  
Nuclease Mapping

Two platelet-derived growth factor A-chain proteins, termed short and long A chains, are generated as a result of alternative mRNA splicing of exon 6 of the A-chain gene. S1 nuclease mapping and polymerase chain reaction analyses demonstrate that both short and long A-chain transcripts are expressed in a variety of normal tissues. In addition, immunohistochemical localization of long A-chain protein reveals a cellular distribution identical to that observed with platelet-derived growth factor heteroserum.

Alternatively spliced platelet-derived growth factor A-chain transcripts are not tumor specific but encode normal cellular proteins

1990 ◽  
Vol 10 (11) ◽  
pp. 6051-6054
Author(s):  
R M Young ◽  
A E Mendoza ◽  
T Collins ◽  
S H Orkin
Keyword(s):  
Growth Factor ◽  
Immunohistochemical Localization ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
S1 Nuclease ◽  
Normal Tissues ◽  
Alternative Mrna Splicing ◽  
Alternatively Spliced ◽  
A Chain ◽  
Nuclease Mapping

Two platelet-derived growth factor A-chain proteins, termed short and long A chains, are generated as a result of alternative mRNA splicing of exon 6 of the A-chain gene. S1 nuclease mapping and polymerase chain reaction analyses demonstrate that both short and long A-chain transcripts are expressed in a variety of normal tissues. In addition, immunohistochemical localization of long A-chain protein reveals a cellular distribution identical to that observed with platelet-derived growth factor heteroserum.

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