Peanut lectin binding properties of germinal centers of mouse lymphoid tissue

Nature ◽  
1980 ◽  
Vol 284 (5754) ◽  
pp. 364-366 ◽  
Author(s):  
M. L. Rose ◽  
M. S. C. Birbeck ◽  
V. J. Wallis ◽  
J. A. Forrester ◽  
A. J. S. Davies
Keyword(s):  
Lymphoid Tissue ◽  
Lectin Binding ◽  
Germinal Centers ◽  
Peanut Lectin ◽  
Binding Properties

The Biology of Germinal Centers in Lymphoid Tissue

1998 ◽  
Author(s):  
G. Jeanette Thorbecke ◽  
Vincent K. Tsiagbe
Keyword(s):  
Lymphoid Tissue ◽  
Germinal Centers

Leguminous glycosidases: Hydrophobic and lectin binding properties

1989 ◽  
Vol 28 (2) ◽  
pp. 355-357 ◽  
Author(s):  
Wolfgang Einhoff ◽  
Harold Rüdiger
Keyword(s):  
Lectin Binding ◽  
Binding Properties

Functional zones of Peyer’s patch (PPs) in rabbits’ caecum

2021 ◽  
Vol 9 (2) ◽  
pp. 82-86
Author(s):  
M. O. Nikitina ◽  
M. V. Kravtsova ◽  
A. A. Bohomaz
Keyword(s):  
Germinal Center ◽  
Lymphoid Tissue ◽  
Experimental Period ◽  
Vermiform Appendix ◽  
Mean Value ◽  
Germinal Centers ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Mantle Zone ◽  
Lymphoid Nodules

A feature of rabbit gut-associated lymphoid tissue is that its structure is more developed than in other animal species. In rabbits it is composed of sacculus rotundus, vermiform appendix and Peyer’s patches. These immune formations contain an organized component of lymphoid tissue – lymphoid nodules (B-cell zone) and interfollicular region (T-cell). Secondary lymphoid nodules with germinal centers presented in them are formed due to antigen stimulation. The caecum of Hyplus rabbits at the age of 30 -, 60 - and 90-days was investigated. Each age group consisted of 5 rabbits. Experimental rabbits are clinically healthy, unvaccinated and untreated against ecto- and endoparasites. Peyer’s patches of the caecum were selected for the study and fixed in 10% of formalin. Subsequently, the specimens stained with hematoxylin-eosin were prepared from the obtained samples. On the 30th day of life, Peyer’s patches in the cecum were detected by gross examination. On the histological level, they had formed interfollicular region and lymphoid nodules. In turn, lymphoid nodules were divided into primary and secondary ones. A well-defined mantle zone and germinal centers were observed in the secondary lymphoid nodules. The regularities of their area indicators increase (mean value, median and interquartile range (IQR)) and their correlation were studied. The most intensive growth of the mantle area and the germinal center was observed from the 30th to the 60th day. The relative area of the mantle zone and the germinal center as part of the secondary lymphoid nodule was determined. Its value did not change during the experimental period.

scholarly journals Germinal centers in human lymph nodes contain reactivated memory B cells

2007 ◽  
Vol 204 (11) ◽  
pp. 2655-2665 ◽  
Author(s):  
Richard J. Bende ◽  
Febe van Maldegem ◽  
Martijn Triesscheijn ◽  
Thera A.M. Wormhoudt ◽  
Richard Guijt ◽  
...  
Keyword(s):  
B Cells ◽  
Lymph Nodes ◽  
Germinal Center ◽  
Lymphoid Tissue ◽  
Somatic Hypermutation ◽  
Germinal Centers ◽  
Memory B Cells ◽  
Cell Clones ◽  
Clonal Origin ◽  
Successive Recall

To reveal migration trails of antigen-responsive B cells in lymphoid tissue, we analyzed immunoglobulin (Ig)M-VH and IgG-VH transcripts of germinal center (GC) samples microdissected from three reactive human lymph nodes. Single B cell clones were found in multiple GCs, one clone even in as many as 19 GCs. In several GCs, IgM and IgG variants of the same clonal origin were identified. The offspring of individual hypermutated IgG memory clones were traced in multiple GCs, indicating repeated engagement of memory B cells in GC reactions. These findings imply that recurring somatic hypermutation progressively drives the Ig repertoire of memory B cells to higher affinities and infer that transforming genetic hits in non-Ig genes during lymphomagenesis do not have to arise during a single GC passage, but can be collected during successive recall responses.

Newly established human pancreatic carcinoma cell lines and their lectin binding properties

1993 ◽  
Vol 13 (1) ◽  
pp. 31-41
Author(s):  
Nobuyuki Nishimura ◽  
Seiji Saito ◽  
Yoshiki Kubota ◽  
Nan-yo Moto-o ◽  
Kuniko Taguchi ◽  
...  
Keyword(s):  
Pancreatic Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Lectin Binding ◽  
Binding Properties ◽  
Carcinoma Cell Lines ◽  
Human Pancreatic Carcinoma Cell

Tissue specific O-linked glycosylation of the neural cell adhesion molecule (N-CAM)

Development ◽  
1989 ◽  
Vol 105 (4) ◽  
pp. 803-811
Author(s):  
F.S. Walsh ◽  
R.B. Parekh ◽  
S.E. Moore ◽  
G. Dickson ◽  
C.H. Barton ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Alternative Splicing ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Lectin Binding ◽  
Peanut Lectin ◽  
3T3 Fibroblasts ◽  
Extracellular Region ◽  
Neural Cell Adhesion ◽  
A Site

We have shown previously that the predominant N-CAM isoform in skeletal muscle myotubes contains as a result of alternative splicing a novel domain (MSD1) in its extracellular region. Here we show that this region represents a site for O-linked carbohydrate attachment. The lipid tailed N-CAM in myotubes was found to bind peanut lectin while the transmembrane isoform from myoblasts lacking MSD1 did not. In addition, N-CAM from a variety of neural sources failed to bind the lectin. Analysis of 3T3 fibroblasts transfected with various N-CAM cDNAs, showed that peanut lectin binding was correlated specifically with the expression of the MSD1 region. The oligosaccharides isolated from a purified preparation of myotube N-CAM were shown to contain an O-linked oligosaccharide whose core structure was a sialylated version of Gal beta 1—3GalNac which is the structure recognized specifically by peanut lectin. These data provide the first evidence for the expression of O-linked carbohydrate on any N-CAM isoform and more specifically target this oligosaccharide to the MSD1 region of myotube N-CAM.

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