Amino acids evoke short-latency membrane conductance increase in pancreatic acinar cells

Nature ◽  
1980 ◽  
Vol 283 (5746) ◽  
pp. 492-494 ◽  
Author(s):  
N. Iwatsuki ◽  
O. H. Petersen
Keyword(s):  
Amino Acids ◽  
Membrane Conductance ◽  
Acinar Cells ◽  
Short Latency ◽  
Pancreatic Acinar Cells ◽  
Conductance Increase

Basic amino acids induce mitochondrial injury in rat pancreatic acinar cells

Pancreatology ◽  
2013 ◽  
Vol 13 (3) ◽  
pp. S5
Author(s):  
Eszter Végh ◽  
Gergő Kovàcs ◽  
Péter Hegyi ◽  
Zoltàn Rakonczay
Keyword(s):  
Amino Acids ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Mitochondrial Injury ◽  
Basic Amino Acids

Actions of a newly isolated intestinal peptide PHI on pancreatic acini

1981 ◽  
Vol 241 (6) ◽  
pp. G498-G502 ◽  
Author(s):  
R. T. Jensen ◽  
K. Tatemoto ◽  
V. Mutt ◽  
G. F. Lemp ◽  
J. D. Gardner
Keyword(s):  
Amino Acids ◽  
Guinea Pig ◽  
Vasoactive Intestinal Peptide ◽  
Acinar Cells ◽  
Effective Concentration ◽  
Pancreatic Acinar Cells ◽  
Amylase Secretion ◽  
Pancreatic Acini ◽  
Apparent Affinity

In dispersed acini from guinea pig pancreas, PHI, a peptide recently isolated from porcine intestine and found to contain 27 amino acids, inhibited binding of 125I-vasoactive intestinal peptide (125I-VIP), increased cellular cAMP, and stimulated amylase secretion. The increase in amylase secretion caused by a maximally effective concentration of PHI in combination with 8-bromo-cAMP, VIP, or secretin was the same as that caused by PHI alone. In contrast, the increase in amylase secretion caused by PHI plus bombesin, carbachol, or the C-terminal octapeptide of cholecystokinin was significantly greater than the sum of the increase caused by each secretagogue acting alone. From the abilities of PHI to inhibit binding of 125I-VIP, to increase cellular cAMP, and to increase amylase secretion, the apparent affinity of PHI for the VIP-preferring receptors on pancreatic acinar cells is approximately 25 times less than that of VIP but 10 times greater than that of secretin. From the ability of PHI to increase cellular cAMP, the apparent affinity of PHI for the secretin-preferring receptors on pancreatic acinar cells is approximately 300 times less than that of secretin but equal to that of VIP.

Development of glycine- and GABA-gated currents in rat spinal motoneurons

1995 ◽  
Vol 74 (1) ◽  
pp. 113-121 ◽  
Author(s):  
B. X. Gao ◽  
L. Ziskind-Conhaim
Keyword(s):  
Amino Acids ◽  
Membrane Conductance ◽  
Gamma Aminobutyric Acid ◽  
Spinal Motoneurons ◽  
Cell Recording ◽  
Inward Currents ◽  
Conductance Changes ◽  
Conductance Increase ◽  
Best Fit ◽  
Reversal Potentials

1. Developmental changes in glycine- and gamma-aminobutyric acid (GABA)-activated currents were studied in spinal motoneurons of embryonic and neonatal rats with the use of whole cell recording techniques. 2. Pressure ejection of glycine or GABA onto motoneuron somata produced Cl(-)-mediated inward currents and membrane depolarizations. During embryonic development, the average amplitude of GABA-gated currents was threefold larger than that of glycine-gated currents, but as a result of a large eightfold postnatal increase in glycine-activated currents, similar currents were produced by both amino acids after birth. 3. At all ages the decay of glycine- and GABA-gated currents best fit one-exponential curve, and their time constants were similar. The average decay time constant decreased by twofold after birth. 4. The ionic specificity of glycine- and GABA-gated channels was studied to determine whether the large amplitude of GABA-activated currents in embryonic motoneurons resulted from the contribution of an outward HCO-3 movement. Manipulations of Cl- and HCO-3 concentrations produced changes in the reversal potentials of glycine and GABA that were similar to the calculated changes in the equilibrium potentials of Cl-. This suggested that glycine- and GABA-gated currents were Cl- specific, and HCO-3 movement did not contribute more to the current generated by GABA than that produced by glycine. 5. Glycine- and GABA-gated currents were associated with severalfold increases in membrane conductance. The conductance increase generated by GABA in embryonic motoneurons was sevenfold larger than that generated by glycine, but similar conductance changes were produced by both amino acids after birth.(ABSTRACT TRUNCATED AT 250 WORDS)

Visualization of CCK-evoked exocytic events in rat pancreatic acinar cells

2001 ◽  
Vol 120 (5) ◽  
pp. A24-A24
Author(s):  
H GAISANO ◽  
L TANG ◽  
L SHEU ◽  
W TRIMBLE
Keyword(s):  
Acinar Cells ◽  
Pancreatic Acinar Cells

Effects of oncogenic K-ras on pancreatic acinar cells In vitro

2001 ◽  
Vol 120 (5) ◽  
pp. A722-A722
Author(s):  
Y BI ◽  
C LOGSDON
Keyword(s):  
Acinar Cells ◽  
Pancreatic Acinar Cells

Carbon tetrachloride induced ultrastructural alterations in pancreatic acinar cells and in the hepatocytes. Similarities and differences

Toxicology ◽  
1978 ◽  
Vol 11 ◽  
pp. 289-296 ◽  
Author(s):  
Carmen R. De Castro ◽  
Adriana S. Bernacchi ◽  
Elida C. De Ferreyra ◽  
Olga M. De Fends ◽  
José A. Castro
Keyword(s):  
Carbon Tetrachloride ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Ultrastructural Alterations ◽  
Similarities And Differences
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