Imidazole inhibits a temperature-dependent component of mammalian skeletal muscle action potential

Nature ◽  
1980 ◽  
Vol 283 (5746) ◽  
pp. 485-486 ◽  
Author(s):  
D. W. Cheung ◽  
E. E. Daniel
Keyword(s):  
Skeletal Muscle ◽  
Action Potential ◽  
Mammalian Skeletal Muscle ◽  
Muscle Action ◽  
Temperature Dependent ◽  
Muscle Action Potential ◽  
Dependent Component

scholarly journals Optical recording of action potential initiation and propagation in mouse skeletal muscle fibers

2018 ◽  
Author(s):  
Q. Banks ◽  
S.J.P. Pratt ◽  
S.R. Iyer ◽  
R.M. Lovering ◽  
E.O. Hernández-Ochoa ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Action Potential ◽  
Conduction Velocity ◽  
Muscle Fibers ◽  
Extracellular Potassium ◽  
Bipolar Electrode ◽  
Muscle Action ◽  
Action Potential Propagation ◽  
Skeletal Muscle Fibers ◽  
Muscle Action Potential

ABSTRACTIndividual skeletal muscle fibers have been used to examine a wide variety of cellular functions and pathologies. Among other parameters, skeletal muscle action potential propagation has been measured to assess the integrity and function of skeletal muscle. In this paper, we utilize Di-8-ANEPPS, a potentiometric dye and mag-fluo-4, a low-affinity intracellular calcium indicator to non-invasively and reliably measure action potential conduction velocity in skeletal muscle. We used an extracellular bipolar electrode to generate an electric field that will initiate an action potential at one end of the fiber or the other. Using enzymatically dissociated flexor digitorum brevis (FDB) fibers, we demonstrate the strength and applicability of this technique. Using high-speed line scans, we estimate the conduction velocity to be approximately 0.4 m/s. In addition to measuring the conduction velocity, we can also measure the passive electrotonic potentials elicited by pulses by either applying tetrodotoxin (TTX) or reducing the bath sodium levels. We applied these methodologies to FDB fibers under elevated extracellular potassium conditions, and found that the conduction velocity is significantly reduced compared to our control concentration. Lastly, we have constructed a circuit model of a skeletal muscle in order to predict passive polarization of the fiber by the field stimuli. Our predictions from the model fiber closely resemble the recordings acquired from in vitro assays. With these techniques, we can examine how many different pathologies and mutations affect skeletal muscle action potential propagation. Our work demonstrates the utility of using Di-8-ANEPPS or mag-fluo-4 to non-invasively measure action potential conduction velocity.

scholarly journals Impact of stimulus duration on motor unit thresholds and alternation in compound muscle action potential scans

2020 ◽  
Author(s):  
Boudewijn T.H.M. Sleutjes ◽  
Janna Ruisch ◽  
Thijs E. Nassi ◽  
Jan R. Buitenweg ◽  
Leonard J. van Schelven ◽  
...  
Keyword(s):  
Action Potential ◽  
Motor Unit ◽  
Stimulus Duration ◽  
Compound Muscle Action Potential ◽  
Muscle Action ◽  
Muscle Action Potential

Intrinsic Reinnervation—Myth or Reality?

1993 ◽  
Vol 18 (4) ◽  
pp. 454-460 ◽  
Author(s):  
R. L. LESTER ◽  
P. J. SMITH ◽  
G. MOTT ◽  
R. M. R. McALLISTER
Keyword(s):  
Action Potential ◽  
Muscle Power ◽  
Muscle Action ◽  
Intrinsic Muscle ◽  
Clinical Recovery ◽  
Muscle Action Potential ◽  
Intrinsic Muscles

A clinical and electromyographic study of major nerve transections at the wrist in 22 patients has shown that electrical recovery nearly always occurs in the intrinsic muscles, despite the absence of clinically detectable function. There appears to be a level of electrical reinnervation above which clinically detectable intrinsic muscle power is usually present. This level would appear to be 50% of the contralateral maximum evoked muscle action potential; above this the “myth” of clinical recovery becomes a reality.

Determination of the best electrode position for recording of the diaphragm compound muscle action potential

2009 ◽  
Vol 40 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Annie Dionne ◽  
Anthony Parkes ◽  
Beata Engler ◽  
Bradley V. Watson ◽  
Michael W. Nicolle
Keyword(s):  
Action Potential ◽  
Compound Muscle Action Potential ◽  
Electrode Position ◽  
Muscle Action ◽  
Muscle Action Potential

Regulation of breathing at beginning of exercise

1963 ◽  
Vol 18 (6) ◽  
pp. 1183-1187 ◽  
Author(s):  
F. N. Craig ◽  
E. G. Cummings ◽  
W. V. Blevins
Keyword(s):  
Action Potential ◽  
Cycle Ergometer ◽  
Respiratory Drive ◽  
Muscle Action ◽  
Work Situation ◽  
Muscle Action Potential ◽  
Regulation Of Breathing ◽  
The Subject ◽  
Leg Movement

In initial 20-sec periods of running on the treadmill, raising the slope to 12% increased by 50% the integrated muscle action potential, recorded from the under side of the thigh, without affecting the ventilation in four men. Five men worked for 1 min at four tasks, two on the treadmill and two on the horizontal cycle ergometer. Each pair of tasks consisted of slow and rapid leg movement at the same rate of work. Ventilation often continued at about the resting rate for the first few seconds of work, unaffected in any significant way by frequency of leg movement. In the subject responding to the onset of exercise by a sudden sharp involuntary increase in ventilation, the nature of the nervous respiratory drive is not clear. Whatever drive arises from the work itself may be strongly modified by training or by complexities in the work situation. leg movement; muscle action potential; nervous respiratory drive Submitted on May 16, 1963

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