scholarly journals Role for the skeletal muscle action potential in non-Hebbian long-term depression at the amphibian (Bufo marinus) neuromuscular junction

Synapse ◽  
2008 ◽  
Vol 62 (4) ◽  
pp. 291-301 ◽  
Author(s):  
Sarah Jane Etherington ◽  
Alan William Everett
Keyword(s):  
Skeletal Muscle ◽  
Neuromuscular Junction ◽  
Action Potential ◽  
Bufo Marinus ◽  
Muscle Action ◽  
Long Term Depression ◽  
Muscle Action Potential

scholarly journals Optical recording of action potential initiation and propagation in mouse skeletal muscle fibers

2018 ◽  
Author(s):  
Q. Banks ◽  
S.J.P. Pratt ◽  
S.R. Iyer ◽  
R.M. Lovering ◽  
E.O. Hernández-Ochoa ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Action Potential ◽  
Conduction Velocity ◽  
Muscle Fibers ◽  
Extracellular Potassium ◽  
Bipolar Electrode ◽  
Muscle Action ◽  
Action Potential Propagation ◽  
Skeletal Muscle Fibers ◽  
Muscle Action Potential

ABSTRACTIndividual skeletal muscle fibers have been used to examine a wide variety of cellular functions and pathologies. Among other parameters, skeletal muscle action potential propagation has been measured to assess the integrity and function of skeletal muscle. In this paper, we utilize Di-8-ANEPPS, a potentiometric dye and mag-fluo-4, a low-affinity intracellular calcium indicator to non-invasively and reliably measure action potential conduction velocity in skeletal muscle. We used an extracellular bipolar electrode to generate an electric field that will initiate an action potential at one end of the fiber or the other. Using enzymatically dissociated flexor digitorum brevis (FDB) fibers, we demonstrate the strength and applicability of this technique. Using high-speed line scans, we estimate the conduction velocity to be approximately 0.4 m/s. In addition to measuring the conduction velocity, we can also measure the passive electrotonic potentials elicited by pulses by either applying tetrodotoxin (TTX) or reducing the bath sodium levels. We applied these methodologies to FDB fibers under elevated extracellular potassium conditions, and found that the conduction velocity is significantly reduced compared to our control concentration. Lastly, we have constructed a circuit model of a skeletal muscle in order to predict passive polarization of the fiber by the field stimuli. Our predictions from the model fiber closely resemble the recordings acquired from in vitro assays. With these techniques, we can examine how many different pathologies and mutations affect skeletal muscle action potential propagation. Our work demonstrates the utility of using Di-8-ANEPPS or mag-fluo-4 to non-invasively measure action potential conduction velocity.

scholarly journals Impact of stimulus duration on motor unit thresholds and alternation in compound muscle action potential scans

2020 ◽  
Author(s):  
Boudewijn T.H.M. Sleutjes ◽  
Janna Ruisch ◽  
Thijs E. Nassi ◽  
Jan R. Buitenweg ◽  
Leonard J. van Schelven ◽  
...  
Keyword(s):  
Action Potential ◽  
Motor Unit ◽  
Stimulus Duration ◽  
Compound Muscle Action Potential ◽  
Muscle Action ◽  
Muscle Action Potential

Intrinsic Reinnervation—Myth or Reality?

1993 ◽  
Vol 18 (4) ◽  
pp. 454-460 ◽  
Author(s):  
R. L. LESTER ◽  
P. J. SMITH ◽  
G. MOTT ◽  
R. M. R. McALLISTER
Keyword(s):  
Action Potential ◽  
Muscle Power ◽  
Muscle Action ◽  
Intrinsic Muscle ◽  
Clinical Recovery ◽  
Muscle Action Potential ◽  
Intrinsic Muscles

A clinical and electromyographic study of major nerve transections at the wrist in 22 patients has shown that electrical recovery nearly always occurs in the intrinsic muscles, despite the absence of clinically detectable function. There appears to be a level of electrical reinnervation above which clinically detectable intrinsic muscle power is usually present. This level would appear to be 50% of the contralateral maximum evoked muscle action potential; above this the “myth” of clinical recovery becomes a reality.

Determination of the best electrode position for recording of the diaphragm compound muscle action potential

2009 ◽  
Vol 40 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Annie Dionne ◽  
Anthony Parkes ◽  
Beata Engler ◽  
Bradley V. Watson ◽  
Michael W. Nicolle
Keyword(s):  
Action Potential ◽  
Compound Muscle Action Potential ◽  
Electrode Position ◽  
Muscle Action ◽  
Muscle Action Potential
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