Ribonuclease activity of wheat leaves and rust infection

Nature ◽  
1974 ◽  
Vol 247 (5442) ◽  
pp. 577-580 ◽  
Author(s):  
ARUN K. CHAKRAVORTY ◽  
MICHAEL SHAW ◽  
LEROY A. SCRUBB
Keyword(s):  
Ribonuclease Activity ◽  
Rust Infection ◽  
Wheat Leaves

Effect of Rust Infection on Oxidative Phosphorylation of Wheat Leaves

Nature ◽  
1958 ◽  
Vol 182 (4650) ◽  
pp. 1686-1687 ◽  
Author(s):  
B. I. POZSÁR ◽  
Z. KIRÁLY
Keyword(s):  
Oxidative Phosphorylation ◽  
Rust Infection ◽  
Wheat Leaves

RIBONUCLEASE ACTIVITY IN RUSTED WHEAT LEAVES

1961 ◽  
Vol 39 (4) ◽  
pp. 775-784 ◽  
Author(s):  
R. Rohringer ◽  
D. J. Samborski ◽  
C. O. Person
Keyword(s):  
Leaf Rust ◽  
Direct Evidence ◽  
Specific Activity ◽  
Host Tissue ◽  
Ribonuclease Activity ◽  
Rnase Activity ◽  
Rnase Inhibitor ◽  
Germination Medium ◽  
Wheat Leaves ◽  
Host Tissues

Extracts from primary leaves of Lee wheat were prepared at various days following inoculation with races of leaf rust and tested for ribonuclease (RNase) activity. As early as 24 hours after inoculation there was a marked increase in the specific activity of the enzyme in extracts of rusted host tissues. A further increase in activity was observed during later stages of infection, with the susceptible and resistant reacting tissue differing only in the degree of their response. Extracts from noninoculated control leaves exhibited a constant RNase activity throughout the period of observation. The germination medium and extracts from germinating uredospores contained comparatively little RNase activity. No direct evidence was obtained either for the possible release of the enzyme from particulate cellular fractions of the host tissue as a result of infection or for the removal of an RNase inhibitor in the host tissue responding to infection.

Evaluating ten spectral vegetation indices for identifying rust infection in individual wheat leaves

2008 ◽  
Vol 10 (6) ◽  
pp. 459-470 ◽  
Author(s):  
R. Devadas ◽  
D. W. Lamb ◽  
S. Simpfendorfer ◽  
D. Backhouse
Keyword(s):  
Vegetation Indices ◽  
Rust Infection ◽  
Spectral Vegetation Indices ◽  
Wheat Leaves

scholarly journals Peroxidase isoenzyme patterns of resistant and susceptible wheat leaves following stem rust infection

1989 ◽  
Vol 111 (3) ◽  
pp. 413-421 ◽  
Author(s):  
BERENIKE E. FLOTT ◽  
BRUNO M. MOERSCHBACHER ◽  
HANS-JOACHIM REISENER
Keyword(s):  
Stem Rust ◽  
Peroxidase Isoenzyme ◽  
Rust Infection ◽  
Wheat Leaves ◽  
Isoenzyme Patterns

THE PHYSIOLOGY OF HOST–PARASITE RELATIONS: VIII. EFFECTS OF RUST INFECTION ON ASCORBIC ACID ANDGLUTATHIONE IN WHEAT LEAVES

1961 ◽  
Vol 39 (6) ◽  
pp. 1327-1336 ◽  
Author(s):  
B. I. Sahai ◽  
Michael Shaw
Keyword(s):  
Ascorbic Acid ◽  
Puccinia Graminis ◽  
Oxidized Glutathione ◽  
Redox Potentials ◽  
Rust Infection ◽  
Puccinia Graminis Tritici ◽  
Auxin Content ◽  
Wheat Leaves ◽  
Host Parasite

First seedling leaves of Little Club (susceptible), Khapli I (moderately susceptible), and Khapli (resistant) wheats were inoculated with Puccinia graminis tritici Erikss. and Henn. (Race 15B). Ascorbic (AA) and dehydroascorbic (DHA) acids and glutathione (GSH) were measured at 1–2 day intervals after inoculation until after sporulation. After infection, AA and DHA increased in Little Club and decreased in Khapli. In Khapli the DHA/AA ratio rose sharply 6–8 days after inoculation; in Little Club a similar rise occurred 15–16 days after inoculation. In Khapli I and Little Club, but not in Khapli, GSH increased sharply when sporulation occurred. AA was not detected in uredospores, but DHA was. Oxidized glutathione was not detected in healthy or infected leaves but was present in uredospores. The results are discussed briefly in relation to the changes in auxin content and redox potentials caused by rust infection.

scholarly journals The Effect of Stem Rust Infection on the Soluble Proteins of Wheat Leaves

1966 ◽  
Vol 19 (5) ◽  
pp. 895 ◽  
Author(s):  
CW Wrigley ◽  
HL Webster
Keyword(s):  
Stem Rust ◽  
Electrophoretic Pattern ◽  
Soluble Proteins ◽  
Wheat Varieties ◽  
Disk Electrophoresis ◽  
Rust Infection ◽  
Electrophoretic Patterns ◽  
Wheat Leaf ◽  
Wheat Leaves

The soluble proteins of wheat leaf and of stem rust uredospores were resolved into about 28 and 34 components, respectively, by disk electrophoresis. The funguscontaining and the fungus-free areas of infected wheat leaves were examined. The electrophoretic pattern of an extract of the lesions was markedly different from the pattern of an extract of uninfected leaves. Comparison with the pattern of an extract of uredospores suggested that some of these differences were due to contamination with fungal proteins. For susceptible varieties, extracts of the extralesion areas of infected leaves showed different electrophoretic patterns from extracts of uninfected leaves. This was not so for resistant wheat varieties.

THE PHYSIOLOGY OF HOST–PARASITE RELATIONS: XIV. THE EFFECT OF RUST INFECTION ON THE NUCLEIC ACID CONTENT OF WHEAT LEAVES

1964 ◽  
Vol 42 (11) ◽  
pp. 1531-1540 ◽  
Author(s):  
W. A. Quick ◽  
Michael Shaw
Keyword(s):  
Dry Weight ◽  
Weight Basis ◽  
Nucleic Acid Content ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Rust Infection ◽  
Dna And Rna ◽  
Fungal Dna ◽  
Wheat Leaves ◽  
Host Parasite

The increase in respiration in rust-infected leaves of Little Club wheat was followed and paralleled by an increase in RNA per gram dry weight. The massive increase in dry weight of infected leaves in the later stages of rust development (9–12 days after inoculation) sometimes obscured the increase in RNA when RNA was expressed on a dry weight but not when it was expressed on a fresh weight basis. There was no increase in RNA in rust-infected Khapli. Rust infection had no effect on DNA per gram dry weight in Little Club or Khapli, but DNA per gram fresh weight increased slightly (15%) in Little Club, indicating the synthesis of fungal DNA. Infection had little effect on the protein content of Little Club but markedly lowered that of Khapli. The results are discussed in relation to earlier cytochemical determinations of DNA and RNA in infected tissue.

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