Effects of Various Substances on DNA Synthesis in Guinea-pig Skin in vitro

Nature ◽  
1967 ◽  
Vol 216 (5116) ◽  
pp. 715-716 ◽  
Author(s):  
PATRICIA R. MANN
Keyword(s):  
Guinea Pig ◽  
Dna Synthesis ◽  
Pig Skin

Effects of selective ultraviolet phototherapy (SUP) and local PUVA treatment on DNA synthesis in guinea pig skin

1980 ◽  
Vol 267 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Helmut Pullmann ◽  
Attila Galosi ◽  
Christian Jakobeit ◽  
Gerd Klaus Steigleder
Keyword(s):  
Guinea Pig ◽  
Dna Synthesis ◽  
Pig Skin ◽  
Puva Treatment

scholarly journals In Vitro Anaphylaxis In Guinea-Pig Skin: Amplification By Burimamide

1976 ◽  
Vol 67 (6) ◽  
pp. 696-699 ◽  
Author(s):  
Shoso Yamamoto ◽  
David Francis ◽  
Malcolm W Greaves
Keyword(s):  
Guinea Pig ◽  
Pig Skin

Release of peptide leukotrienes(LTS) from sensitized guinea pig skin in in vitro cutaneous anaphylactic reactions

1991 ◽  
Vol 2 (3) ◽  
pp. 232
Author(s):  
Tsunemi Numata ◽  
Kiyoshi Furutani ◽  
Isamu Kimura ◽  
Satoru Yamada ◽  
Shoso Yamamoto
Keyword(s):  
Guinea Pig ◽  
Pig Skin

The interaction between tetracycline and reconstituted guinea-pig-skin collagen in vitro

1964 ◽  
Vol 86 (3) ◽  
pp. 579-587 ◽  
Author(s):  
Richard Jacobs ◽  
William H. Harris ◽  
Elton P. Katz ◽  
Melvin J. Glimcher
Keyword(s):  
Guinea Pig ◽  
Pig Skin ◽  
Skin Collagen

scholarly journals Toxicity and medical countermeasure studies on the organophosphorus nerve agents VM and VX

2015 ◽  
Vol 471 (2176) ◽  
pp. 20140891 ◽  
Author(s):  
Helen Rice ◽  
Christopher H. Dalton ◽  
Matthew E. Price ◽  
Stuart J. Graham ◽  
A. Christopher Green ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Penetration Rate ◽  
Lethal Dose ◽  
Scientific Advice ◽  
Complete Protection ◽  
Pig Skin ◽  
Syrian Arab Republic ◽  
Medical Countermeasures

To support the effort to eliminate the Syrian Arab Republic chemical weapons stockpile safely, there was a requirement to provide scientific advice based on experimentally derived information on both toxicity and medical countermeasures (MedCM) in the event of exposure to VM, VX or VM–VX mixtures. Complementary in vitro and in vivo studies were undertaken to inform that advice. The penetration rate of neat VM was not significantly different from that of neat VX, through either guinea pig or pig skin in vitro . The presence of VX did not affect the penetration rate of VM in mixtures of various proportions. A lethal dose of VM was approximately twice that of VX in guinea pigs poisoned via the percutaneous route. There was no interaction in mixed agent solutions which altered the in vivo toxicity of the agents. Percutaneous poisoning by VM responded to treatment with standard MedCM, although complete protection was not achieved.

Effects of ACTH and ACTH fragments on DNA synthesis in guinea-pig adrenal segments kept in organ culture

1987 ◽  
Vol 115 (3) ◽  
pp. 505-510 ◽  
Author(s):  
N. M. Wulffraat ◽  
H. A. Drexhage ◽  
P. Jeucken ◽  
R. D. van der Gaag ◽  
W. M. Wiersinga
Keyword(s):  
Guinea Pig ◽  
Organ Culture ◽  
Dna Synthesis ◽  
Intermediate Lobe ◽  
Terminal Part ◽  
Terminal Fragment ◽  
Acth Fragments ◽  
Epidermal Growth ◽  
Stimulation Of

ABSTRACT Stimulation of adrenal DNA synthesis by ACTH(1–39) and its fragments ACTH(1–24) (Synacthen) and ACTH(18–39) was investigated. Synthesis of DNA was measured as the increase in the percentage of cells in S-phase (Feulgen densitometry) in guinea-pig adrenal explants kept in organ culture and exposed to the peptides for 5 h at 37 °C. ACTH(1–39) and its C-terminal fragment ACTH(18–39) (corticotrophin-like intermediate lobe peptide) were found to be potent stimulators of in-vitro adrenal DNA synthesis. The dose–response kinetics were biphasic and optimal responsiveness was reached in both instances at 1 fmol/1–10 pmol/l (this biological effect of ACTH(18–39) has hitherto not been described). The N-terminal fragment ACTH(1–24) gave only minimal responses. Thyrotrophin and LH, tested as controls, did not induce adrenal DNA synthesis. Epidermal growth factor was a potent stimulator of adrenal DNA synthesis in vitro. Our data suggest a trophic action of the C-terminal part (ACTH(18–39)) of the corticotrophic molecule. Clear trophic effects were also found for the N-terminal part of the pro-opiomelanocortin molecule N-POC(1–76) (optimum 0·1 nmol/l) and N-POC(51– 62) (optimum 0·1 pmol/l). The latter observations support earlier concepts that this part of the proopiomelanocortin molecule has a stimulatory effect on adrenal DNA synthesis. J. Endocr. (1987) 115, 505–510

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