Mitotic Activity in the Chick Embryo Epidermis

Nature ◽  
1967 ◽  
Vol 216 (5122) ◽  
pp. 1352-1352 ◽  
Author(s):  
SIMON ROTHBERG ◽  
THOMAS M. EKEL
Keyword(s):  
Chick Embryo ◽  
Mitotic Activity

Action of nuclear and cytoplasmic fractions of liver homogenate on liver growth in the chick embryo

Development ◽  
1968 ◽  
Vol 20 (1) ◽  
pp. 53-71
Author(s):  
G. D. Tumanishvili ◽  
Naira V. Salamatina
Keyword(s):  
Cell Division ◽  
Chick Embryo ◽  
Mitotic Activity ◽  
Liver Homogenate ◽  
Liver Growth ◽  
Organ Growth ◽  
Tissue Volume ◽  
Number Of Cells

A number of observations have been made during a study of the regulation of organ growth which lead to the conclusion that there is a certain correlation between the density of cell packing and mitotic activity in the tissue (Tumanishvili, 1964, 1965a, b; Tumanishvili & Tabidze, 1962, 1963). The density of cell packing is expressed as the concentration of nuclei (CN), reflecting not only the number of cells in a given tissue volume, but an aspect of interrelation of nuclei and cytoplasm as well. Observations have shown that an increase in mitotic activity always leads to an increase in the concentration of nuclei, and a decrease to a decrease. At the same time an increase in the concentration of nuclei appears to cause suppression of mitotic activity, while a decrease of the nuclear concentration to cause vigorous cell division.

Stimulation of DNA synthesis and mitotic activity of chick embryo hepatocytes in primary culture

In Vitro ◽  
1984 ◽  
Vol 20 (3) ◽  
pp. 172-182 ◽  
Author(s):  
Hans Hirsiger ◽  
Urs Giger ◽  
Urs A. Meyer
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Primary Culture ◽  
Mitotic Activity ◽  
Stimulation Of

Sclerotome Mitotic Activity in Stage 19 Chick Embryo Braquial Somites

1997 ◽  
Vol 3 (S2) ◽  
pp. 179-180
Author(s):  
M. González-Santander Martinez ◽  
M. Monteagudo de la Rosa ◽  
G. Martinez Cuadrado ◽  
R. González Santander
Keyword(s):  
Extracellular Matrix ◽  
Cell Differentiation ◽  
Chick Embryo ◽  
Mitotic Activity ◽  
Toluidine Blue ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Chick Embryos ◽  
White Leghorn ◽  
Intercellular Spaces

Somite disintegration begins with the disruption and dispersion of cells in its ventromedial region, that upon detaching from the somite form a defined group of cells called “sclerotome”. The sclerotome is formed by scleroblasts that originate paraxial segments. Scleroblasts disperse and migrate forming the “sclerotomal mesenchyma”. This sclerotomal mesenchyma, in its evolution and cell differentiation, will give rise to skeletal tissues. One of the first features of the scleroblast is the production of extracellular matrix rich in hialuronic acid that facilitates the spreading of filopodia and the migration of the sclerotome. Sclerotome development is closely associated to the microenvironment created by the extracellular matrix in which scleroblasts are located. Hialuronic acid is found in the ample intercellular spaces occupied by the hydrated matrix.White Leghorn chick embryos at H.H. stages 9 to 12 were fixed in 2.5% glutaraldehyde, postfixed in 1 % osmium tetroxide and embedded in araldite. Semithin transversal sections were stained with toluidine blue and visualized under the light microscope to locate the sclerotome.

Mitotic activity during somite segmentation in the early chick embryo

1984 ◽  
Vol 169 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Claudio D. Stern ◽  
Ruth Bellairs
Keyword(s):  
Chick Embryo ◽  
Mitotic Activity ◽  
Early Chick Embryo

Otoconia formation in the chick embryo

1983 ◽  
Vol 41 ◽  
pp. 572-573
Author(s):  
C.D. Fermin ◽  
M. Igarashi
Keyword(s):  
Chick Embryo ◽  
Inner Ear ◽  
Gallus Domesticus ◽  
The Body ◽  
Abnormal Behavior ◽  
Intensive Study ◽  
Basic Fuchsin ◽  
Gestational Period ◽  
Sensory Epithelia ◽  
Transmission Electron

Otoconia are microscopic geometric structures that cover the sensory epithelia of the utricle and saccule (gravitational receptors) of mammals, and the lagena macula of birds. The importance of otoconia for maintanance of the body balance is evidenced by the abnormal behavior of species with genetic defects of otolith. Although a few reports have dealt with otoconia formation, some basic questions remain unanswered. The chick embryo is desirable for studying otoconial formation because its inner ear structures are easily accessible, and its gestational period is short (21 days of incubation).The results described here are part of an intensive study intended to examine the morphogenesis of the otoconia in the chick embryo (Gallus- domesticus) inner ear. We used chick embryos from the 4th day of incubation until hatching, and examined the specimens with light (LM) and transmission electron microscopy (TEM). The embryos were decapitated, and fixed by immersion with 3% cold glutaraldehyde. The ears and their parts were dissected out under the microscope; no decalcification was used. For LM, the ears were embedded in JB-4 plastic, cut serially at 5 micra and stained with 0.2% toluidine blue and 0.1% basic fuchsin in 25% alcohol.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

Scanning electron microscopic study of collagen fibrillogenesis and extracellular compartmentalization in the chick embryo tendon

1986 ◽  
Vol 44 ◽  
pp. 208-209
Author(s):  
Grace C.H. Yang
Keyword(s):  
Chick Embryo ◽  
Extracellular Space ◽  
Fibroblast Cell ◽  
Collagen Fibrils ◽  
Electron Microscopic ◽  
Voltage Electron ◽  
Scanning Electron Microscopic Study ◽  
Microscopic Studies ◽  
And Function

The size and organization of collagen fibrils in the extracellular matrix is an important determinant of tissue structure and function. The synthesis and deposition of collagen involves multiple steps which begin within the cell and continue in the extracellular space. High-voltage electron microscopic studies of the chick embryo cornea and tendon suggested that the extracellular space is compartmentalized by the fibroblasts for the regulation of collagen fibril, bundle, and tissue specific macroaggregate formation. The purpose of this study is to gather direct evidence regarding the association of the fibroblast cell surface with newly formed collagen fibrils, and to define the role of the fibroblast in the control and the precise positioning of collagen fibrils, bundles, and macroaggregates during chick tendon development.

An adrenocortical oncocytoma with unusual mitochondrial inclusions and cytoplasmic crystals

1994 ◽  
Vol 52 ◽  
pp. 250-251
Author(s):  
W.T. Gunning ◽  
G.D. Haselhuhn ◽  
E.R. Phillips ◽  
S.H. Selman
Keyword(s):  
Adrenal Gland ◽  
Salivary Glands ◽  
Mitotic Activity ◽  
Diagnostic Criteria ◽  
Vascular Invasion ◽  
Benign Neoplasm ◽  
Benign Tumors ◽  
Nuclear Morphology ◽  
Case Presentation ◽  
Microscopic Evidence

Within the last few years, adrenal cortical tumors with features concordant with the diagnostic criteria attributed to oncocytomas have been reported. To date, only nine reported cases exist in the literature. This report is the tenth case presentation of a presumptively benign neoplasm of the adrenal gland with a rare differentiation. Oncocytomas are well recognized benign tumors of the thyroid, parathyroid, and salivary glands and of the kidney. Other organs also give rise to these types of tumors, however with less frequency than the former sites. The characteristics generally used to classify a tumor as an oncocytoma include the following criteria: the tumor is 1) usually a solitary circumscribed mass with no gross nor microscopic evidence of metastasis (no tissue nor vascular invasion), 2) fairly bland in terms of mitotic activity and nuclear morphology, and 3) composed of large eosinophillic cells in which the cytoplasm is packed full of mitochondria (Figure 1).

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