Stimulation of DNA synthesis and mitotic activity of chick embryo hepatocytes in primary culture

In Vitro ◽  
1984 ◽  
Vol 20 (3) ◽  
pp. 172-182 ◽  
Author(s):  
Hans Hirsiger ◽  
Urs Giger ◽  
Urs A. Meyer
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Primary Culture ◽  
Mitotic Activity ◽  
Stimulation Of

Growth Hormone-Dependent Serum Stimulation of DNA Synthesis in Chick Embryo Fibroblasts in Culture12

Endocrinology ◽  
1975 ◽  
Vol 96 (1) ◽  
pp. 193-198 ◽  
Author(s):  
KENNETH L. COHEN ◽  
PATRICIA A. SHORT ◽  
S. PETER NISSLEY
Keyword(s):  
Growth Hormone ◽  
Chick Embryo ◽  
Dna Synthesis ◽  
Serum Stimulation ◽  
Embryo Fibroblasts ◽  
Stimulation Of

scholarly journals Differential effect of hormones on macromolecular synthesis and mitosis in chick embryo cells.

1975 ◽  
Vol 67 (2) ◽  
pp. 492-498 ◽  
Author(s):  
J B Baseman ◽  
N S Hayes
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Metabolic Responses ◽  
Resting Cells ◽  
Cell Heterogeneity ◽  
Macromolecular Synthesis ◽  
Maximal Stimulation ◽  
Fresh Serum ◽  
Embryo Cells ◽  
Stimulation Of

Exposure of serum-deprived confluent monolayers of chick embryo cells to fresh serum results in maximal stimulation of synthesis of RNA and protein followed by increased DNA synthesis and mitosis. The addition of insulin to quiescent cultures effects a similar acceleration of synthesis of RNA and protein, but little stimulation of DNA synthesis and mitosis is evident. However, the simultaneous addition of insulin and hydrocortisone to resting cells causes a significant increase in the rate of DNA synthesis although the level reached is considerably lower than that obtained with serum and still no mitosis occurs. Unexpectedly, insulin plus hydrocortisone prevents maximal synthesis of RNA and protein in contrast to insulin-treated cultures. Nuclear autoradiography and percent mitosis of cells incubated with various regulatory factors indicate that cell heterogeneity exists and is reflected in the metabolic responses of subpopulations to specific regulatory signals.

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