Sodium Transport and Specific Antiserum

Nature ◽  
1967 ◽  
Vol 215 (5102) ◽  
pp. 739-741
Author(s):  
GERONIMO TERRES ◽  
JOHN X. LOUGHRAN
Keyword(s):  
Sodium Transport ◽  
Specific Antiserum

Use of Uranium-Labeled Antibodies for Electron Microscopic Localization of Various Antigens in Mammalian Cells

1967 ◽  
Vol 25 ◽  
pp. 136-137
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger
Keyword(s):  
Endoplasmic Reticulum ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Specific Antiserum ◽  
Electron Microscopic ◽  
E Coli ◽  
Cytoplasmic Membranes ◽  
Viral Progeny ◽  
Ultrathin Sections ◽  
Electron Microscopic Localization

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.

Female sex steroids modulate alveolar epithelial sodium transport

2010 ◽  
Vol 222 (S 01) ◽  
Author(s):  
M Laube ◽  
E Küppers ◽  
U Thome
Keyword(s):  
Sex Steroids ◽  
Sodium Transport ◽  
Alveolar Epithelial ◽  
Female Sex

INFLUENCE OF SPECIFIC ANTISERUM ON BIOLOGICAL EFFECTS OF HUMAN GONADOTROPHINS

1967 ◽  
Vol 56 (1_Suppl) ◽  
pp. S122
Author(s):  
P.-J. Czygan ◽  
D. Krebs ◽  
F. Lehmann ◽  
G. Bettendorf
Keyword(s):  
Biological Effects ◽  
Specific Antiserum

Erythrocyte Sodium Transport, Intraplatelet pH, and Calcium Concentration in Salt-Sensitive Hypertension

Hypertension ◽  
1996 ◽  
Vol 27 (4) ◽  
pp. 919-925 ◽  
Author(s):  
María del Mar Lluch ◽  
Alejandro de la Sierra ◽  
Esteban Poch ◽  
Antonio Coca ◽  
María Teresa Aguilera ◽  
...  
Keyword(s):  
Sodium Transport ◽  
Calcium Concentration ◽  
Erythrocyte Sodium ◽  
Salt Sensitive Hypertension
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