scholarly journals Nedd4-2 and the Regulation of Epithelial Sodium Transport

2012 ◽  
Vol 3 ◽  
Author(s):  
Daniela Rotin ◽  
Olivier Staub
Keyword(s):  
Sodium Transport

Female sex steroids modulate alveolar epithelial sodium transport

2010 ◽  
Vol 222 (S 01) ◽  
Author(s):  
M Laube ◽  
E Küppers ◽  
U Thome
Keyword(s):  
Sex Steroids ◽  
Sodium Transport ◽  
Alveolar Epithelial ◽  
Female Sex

Erythrocyte Sodium Transport, Intraplatelet pH, and Calcium Concentration in Salt-Sensitive Hypertension

Hypertension ◽  
1996 ◽  
Vol 27 (4) ◽  
pp. 919-925 ◽  
Author(s):  
María del Mar Lluch ◽  
Alejandro de la Sierra ◽  
Esteban Poch ◽  
Antonio Coca ◽  
María Teresa Aguilera ◽  
...  
Keyword(s):  
Sodium Transport ◽  
Calcium Concentration ◽  
Erythrocyte Sodium ◽  
Salt Sensitive Hypertension

scholarly journals Imaging Salt Uptake Dynamics in Plants Using PET

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gerard Ariño-Estrada ◽  
Gregory S. Mitchell ◽  
Prasenjit Saha ◽  
Ahmad Arzani ◽  
Simon R. Cherry ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Sodium Transport ◽  
Crop Production ◽  
Small Animal ◽  
Region Of Interest ◽  
Salt Transport ◽  
Small Animal Pet ◽  
Transport Dynamics ◽  
Positron Emission ◽  
Over Time

AbstractSoil salinity is a global environmental challenge for crop production. Understanding the uptake and transport properties of salt in plants is crucial to evaluate their potential for growth in high salinity soils and as a basis for engineering varieties with increased salt tolerance. Positron emission tomography (PET), traditionally used in medical and animal imaging applications for assessing and quantifying the dynamic bio-distribution of molecular species, has the potential to provide useful measurements of salt transport dynamics in an intact plant. Here we report on the feasibility of studying the dynamic transport of 22Na in millet using PET. Twenty-four green foxtail (Setaria viridis L. Beauv.) plants, 12 of each of two different accessions, were incubated in a growth solution containing 22Na+ ions and imaged at 5 time points over a 2-week period using a high-resolution small animal PET scanner. The reconstructed PET images showed clear evidence of sodium transport throughout the whole plant over time. Quantitative region-of-interest analysis of the PET data confirmed a strong correlation between total 22Na activity in the plants and time. Our results showed consistent salt transport dynamics within plants of the same variety and important differences between the accessions. These differences were corroborated by independent measurement of Na+ content and expression of the NHX transcript, a gene implicated in sodium transport. Our results demonstrate that PET can be used to quantitatively evaluate the transport of sodium in plants over time and, potentially, to discern differing salt-tolerance properties between plant varieties. In this paper, we also address the practical radiation safety aspects of working with 22Na in the context of plant imaging and describe a robust pipeline for handling and incubating plants. We conclude that PET is a promising and practical candidate technology to complement more traditional salt analysis methods and provide insights into systems-level salt transport mechanisms in intact plants.

11-Dehydrocorticosterone, a glucocorticoid metabolite, inhibits aldosterone action in toad bladder

1991 ◽  
Vol 261 (5) ◽  
pp. F873-F879 ◽  
Author(s):  
A. S. Brem ◽  
K. L. Matheson ◽  
J. L. Barnes ◽  
D. J. Morris
Keyword(s):  
Sodium Transport ◽  
Cell Layer ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Hydroxysteroid Dehydrogenase ◽  
Toad Bladder ◽  
Compound A ◽  
Dose Dependent Fashion ◽  
Epithelial Cell Layer ◽  
Dose Dependent

The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) metabolizes glucocorticoid hormones and diminishes their ability to induce sodium transport. In these studies, we determined the location of this enzyme in toad bladder and assessed the biological role for its 11-dehydro end product. Employing a polyclonal antibody directed toward 11 beta-OHSD and immunofluorescence techniques, we located the enzyme in the epithelial cell layer of the toad bladder. Although corticosterone (10(-7) M) can partially suppress aldosterone (10(-7) M)-stimulated short-circuit current (SCC), a clear excess of corticosterone (10(-6) M) did not inhibit the aldosterone-induced induced (10(-8) M) rise in SCC (n = 6). The 11-dehydro product of corticosterone, 11-dehydrocorticosterone (compound A) added to the serosal bath suppressed aldosterone (10(-8) M) peak SCC (360 min) in a dose-dependent fashion reaching 46 +/- 5% of control values at 10(-5) M (n = 6; P less than 0.001). Compound A (10(-5) M) in the mucosal bath also was capable of partially inhibiting the peak aldosterone rise in SCC to 63 +/- 7% of control values with aldosterone at 10(-8) M (n = 6; P less than 0.01) and to 64 +/- 10% of control values with aldosterone at 10(-7) M (n = 9; P less than 0.01). Compound A alone at 10(-5) M did not have any effect on SCC. Isolated toad bladders were not able to transform compound A (at 10(-8) and 10(-5) M) back to corticosterone. Thus the 11-dehydro end product of 11 beta-OHSD (compound A) may play a biologic role by regulating a component of mineralocorticoid-induced sodium transport.

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