A Culture Method of growing Entamoeba histolytica and Other Anaerobic Amoebae for the Study of their Nuclear Division

Nature ◽  
1966 ◽  
Vol 211 (5052) ◽  
pp. 992-992 ◽  
Author(s):  
J. P. DUBEY ◽  
S. R. DAS
Keyword(s):  
Entamoeba Histolytica ◽  
Nuclear Division ◽  
Culture Method

scholarly journals THE ENCYSTMENT OF DYSENTERY AMEBÆ IN VITRO

1918 ◽  
Vol 28 (4) ◽  
pp. 387-413 ◽  
Author(s):  
Kazuyoshi Yoshida
Keyword(s):  
Entamoeba Histolytica ◽  
Nuclear Division ◽  
Intermediate Stage ◽  
The Other ◽  
Central Vacuole ◽  
Transitional Form ◽  
Vegetative Form ◽  
Other Hand ◽  
Favorable Conditions

Dysentery amebæ may live for 72 hours in vitro. The transition of the large vegetative forms into the cyst may be observed in vitro. The large vegetative ameba always passes into an intermediate stage before assuming the small vegetative form. The atypical forms are dysentery amebas with abnormal nuclei and cytoplasm. When circumstances are unfavorable encystment takes place. Under favorable conditions, on the other hand, they may again change into typical forms. The majority of the "degenerative" forms of Hartmann cannot be so classed. According to our observation, they represent forms which have been described here. Hartmann's Entamœba histolytica is really a typical transitional form of Entamœba tetragena. The origin and significance of the central vacuole have been elucidated. Nuclear division of the dysentery ameba proceeds in three ways. The typical forms divide on the whole mitotically, and the atypical a mitotically or in a primitively mitotic manner.

New observations of the nuclear division in Entamoeba histolytica. The chromosomes

1997 ◽  
Vol 89 (7) ◽  
pp. 475-480 ◽  
Author(s):  
Francisco J. Solis ◽  
Henry P. Adams
Keyword(s):  
Entamoeba Histolytica ◽  
Nuclear Division

Entamoeba histolytica: Cell cycle and nuclear division

1988 ◽  
Vol 67 (1) ◽  
pp. 85-95 ◽  
Author(s):  
Esther Orozco ◽  
Francisco Javier Solís ◽  
Jorge Domínguez ◽  
Bibiana Chávez ◽  
Fidel Hernández
Keyword(s):  
Cell Cycle ◽  
Entamoeba Histolytica ◽  
Nuclear Division

Structural Organization of Gamma-Tubulin in the Microtubule Organizing Center (MTOC) During the Nuclear Division of Entamoeba histolytica Trophozoites

2000 ◽  
Vol 31 (4) ◽  
pp. S205-S206 ◽  
Author(s):  
Eduardo Gómez-Conde ◽  
M.C López–Robles ◽  
R Hernández-Rivas ◽  
P Hernández-Jáuregui ◽  
Miguel Vargas-Mejı́a
Keyword(s):  
Entamoeba Histolytica ◽  
Structural Organization ◽  
Nuclear Division ◽  
Microtubule Organizing Center ◽  
Organizing Center ◽  
Gamma Tubulin

Erythrophagocytosis by Entamoeba histolytica.- Ultrastructural Study

1972 ◽  
Vol 30 ◽  
pp. 156-157 ◽  
Author(s):  
Norberto Treviño ◽  
Alfredo Feria-Velasco ◽  
I. Ruiz de Chávez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Entamoeba Histolytica ◽  
Ultrastructural Study ◽  
Saline Solution ◽  
Physiological Saline ◽  
Ultrastructural Level ◽  
Hot Plate ◽  
Physiological Saline Solution ◽  
Axenic Conditions

Although erythrophagocytosis by various species of Entamoeba is a well known phenomenon this has not yet been studied in detail at the ultrastructural level. The present work deals with the description of the incorporation process of erythrocytes by trophozoites of E. histolytica. For this study, trophozoites of E. histolytica, HK-9:NIH strain cultured in axenic conditions and washed human erythrocytes were placed on a hot plate at 37°C in physiological saline solution. After 5 minutes, 2.5% glutarldehyde was added and the samples were processed according to conventional techniques for electron microscopy.Based upon light microscopy studies on living trophozoites in contact with erythrocytes, it seems that erythrophagocytosis only takes place in one pole of the parasite.

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

“Blastosphere”: A new culture method for human fetal hepatic progenitor cells

2001 ◽  
Vol 120 (5) ◽  
pp. A542-A543
Author(s):  
T HIROSE ◽  
K YASUCHIKA ◽  
T FUJIKAWA ◽  
H FUJII ◽  
S OE ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Culture Method ◽  
Hepatic Progenitor Cells
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