A Culture Method for Growing Small Free-Living Amœbæ for the Study of their Nuclear Division

Nature ◽  
1950 ◽  
Vol 165 (4185) ◽  
pp. 65-66 ◽  
Author(s):  
B. N. SINGH
Keyword(s):  
Nuclear Division ◽  
Culture Method ◽  
Free Living

scholarly journals Investigating the frequency of Free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020

2020 ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective: These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results: Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enabled us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study.

scholarly journals Investigating the Frequency of Free-living Amoeba in Water Resources of Bandar Abbas City, Hormozgan Province, Iran 2019-2020

2020 ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency. Results Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enables us to distinguish two genotypes of T4 and T15 in this study.

scholarly journals Investigating the frequency of Free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020

2020 ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective: These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results: Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enabled us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study.

scholarly journals Nuclear division in nine species of small free-living amoebae and its bearing on the classification of the order Amoebida

1952 ◽  
Vol 236 (636) ◽  
pp. 405-461 ◽  
Keyword(s):  
Nuclear Division ◽  
New Method ◽  
Free Living ◽  
Cultural Conditions ◽  
Naegleria Gruberi

A study of nine species of small free-living amoebae has been made under standardized and reproducible cultural conditions, by a new method that enables specimens in all stages of division to be obtained easily. In all species the resting nucleus shows a Feulgen-negative nucleolus and Feulgen-positive chromatin granules. Nuclear division in these species and in other amoebae described by other workers is of two main types on which it is proposed to create two new families— Schizopyrenidae and Hartmannellidae. In Schizopyrenidae, the type genus Schizopyrenus n.g. and two other genera, Naegleria and Didascalus n.g., are defined. Naegleria gruberi, Didascalus thorntoni n.sp., Schizopyrenus russelli n.sp., S. erythaenusa n.sp. and S. atopus n.sp. are described. In Hartmannellidae the type genus Hartmannella is defined. H. glebae, H. rhysodes n.sp., H. leptocnemus n.sp and H. agricola are described. The relation of the proposed classification to previously defined families and genera of amoebae, and its bearing on phylogeny are discussed.

scholarly journals Investigating the frequency of Free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020

2020 ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective: These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results: Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enables us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study.

scholarly journals Investigating the frequency of free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019–2020

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enabled us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study.

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