scholarly journals A Method of Isolating and Counting Nippostrongylus brasiliensis from Unweaned Rats

Nature ◽  
1966 ◽  
Vol 209 (5023) ◽  
pp. 630-630 ◽  
Author(s):  
PAUL WHUR ◽  
MARY GRACIE
Keyword(s):  
Nippostrongylus Brasiliensis

scholarly journals Resistance of Cotton Rats, Sigmodon hispidus, to Primary Infection by Nippostrongylus brasiliensis

2002 ◽  
Vol 64 (5) ◽  
pp. 423-426 ◽  
Author(s):  
Hendra WIBAWA ◽  
Deni NOVIANA ◽  
Kimiyuki TSUCHIYA ◽  
Fadjar SATRIJA ◽  
Yoichiro HORII
Keyword(s):  
Primary Infection ◽  
Nippostrongylus Brasiliensis ◽  
Sigmodon Hispidus ◽  
Cotton Rats

The cuticle of adultNippostrongylus brasiliensis

Parasitology ◽  
1965 ◽  
Vol 55 (1) ◽  
pp. 173-181 ◽  
Author(s):  
D. L. Lee
Keyword(s):  
Electron Microscope ◽  
Body Wall ◽  
Technical Assistance ◽  
Cortical Layer ◽  
The Body ◽  
Collagen Fibrils ◽  
Intestinal Cells ◽  
Nippostrongylus Brasiliensis ◽  
Normal Appearance

The cuticle of adults ofNippostrongylus brasiliensishas been described using histological, histochemical and ultrastructural techniques.The cuticle has the following layers: an outer triple-layered membrane; a single cortical layer; a fluid-filled layer which is traversed by numerous collagen fibrils; struts which support the fourteen longitudinal ridges of the cuticle and which are suspended by collagen fibrils in the fluid-filled layer; two fibre layers, each layer apparently containing three layers of fibres; and a basement lamella.The fluid-filled layer contains haemoglobin and esterase.The muscles of the body wall are attached to either the basement lamella or to the fibre layers of the cuticle.The mitochondria of the hypodermis are of normal appearance.The longitudinal ridges of the cuticle appear to abrade the microvilli of the intestinal cells of the host.Possible functions of the cuticle are discussed.I wish to thank Dr P. Tate, in whose department this work was done, for helpful suggestions and criticism at all stages of this work, and Mr A. Page for technical assistance. I also wish to thank Professor Boyd for permission to use the electron microscope in the Department of Anatomy.

Studies on the aggregation behaviour of Nippostrongylus brasiliensis

Parasitology ◽  
1971 ◽  
Vol 63 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Thomas J. W. Alphey
Keyword(s):  
Research Council ◽  
Sexual Attraction ◽  
Scientific Research ◽  
Nippostrongylus Brasiliensis ◽  
T Tube ◽  
Aggregation Behaviour ◽  
Choice Chamber

Two behaviour patterns which could bring about clumping in N. brasiliensis, sexual attraction and thigmokinesis, were investigated. A T-tube choice-chamber to facilitate an in vitro study of sexual attraction is described. A method for studying aggregation is also outlined. Male N. brasiliensis were shown to be attracted to female worms, positive sexual attraction. Both sexes were shown to exhibit strong thigmokinetic responses, and possible functions of such behaviour are discussed.I am grateful to Professors J. Brough and D. Bellamy for providing the facilities during the course of this study. I should also like to thank Dr E.. A. Hammond for his supervision, help and criticism throughout the project. This work was carried out during the tenure of a Scientific Research Council Studentship.

scholarly journals Molecular cloning of mouse intestinal trefoil factor and its expression during goblet cell changes

1995 ◽  
Vol 311 (1) ◽  
pp. 293-297 ◽  
Author(s):  
M Tomita ◽  
H Itoh ◽  
N Ishikawa ◽  
A Higa ◽  
H Ide ◽  
...  
Keyword(s):  
Goblet Cell ◽  
Goblet Cells ◽  
Recovery Phase ◽  
Nippostrongylus Brasiliensis ◽  
Trefoil Factor ◽  
Intestinal Trefoil Factor ◽  
Cell Hyperplasia ◽  
Reverse Transcription Pcr ◽  
Rapid Amplification ◽  
Mitf Expression

A cDNA encoding mouse intestinal trefoil factor (mITF) was successfully cloned and sequenced from the small intestine of C57BL/6 mouse by using the combination of reverse transcription-PCR and rapid amplification of cDNA ends methods. The gene was, similar to rat and human ITFs, mainly expressed in the small and large intestine. The mITF expression was up-regulated during the recovery phase after depletion of goblet cells in acetic acid-induced colitis. On the other hand, the expression in the jejunum was not altered, while goblet cell hyperplasia was induced by Nippostrongylus brasiliensis infection. These results suggest that the mITF expression did not simply correlate with the number of goblet cells. The mITF may play an important role in the maintenance and repair of mucosal function of the rectum. Additionally, the mITF in the jejunum may play a role in alteration of the physicochemical nature of goblet cell mucins, thereby affecting the establishment of intestinal helminths.

scholarly journals The effects of changes in nutritional demand on gastrointestinal parasitism in lactating rats

2007 ◽  
Vol 97 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Heidi Normanton ◽  
Jos G. M. Houdijk ◽  
Neil S. Jessop ◽  
Dave P. Knox ◽  
Ilias Kyriazakis
Keyword(s):  
Crude Protein ◽  
Inflammatory Cells ◽  
Host Immunity ◽  
Nippostrongylus Brasiliensis ◽  
Protein Food ◽  
Treatment Groups ◽  
Nutrient Demand ◽  
Low Protein ◽  
Ad Libitum ◽  
Gastrointestinal Parasitism

Lactating rats experience a breakdown of immunity to parasites, i.e. they carry larger worm burdens after re-infection compared to their non-lactating counterparts. Feeding high-protein foods to lactating rats results in reduced worm burdens. This could be attributed to changes in gastrointestinal environment or to overcoming effects of nutrient scarcity on host immunity. The latter hypothesis was addressed through a manipulation of nutrient demand by manipulating litter size. Twenty-three rats were immunized prior to mating and re-infected on day 2 of lactation with 1600 infective Nippostrongylus brasiliensis larvae. From parturition onwards, rats received ad libitum a low-protein food (100 g crude protein/kg). Litter sizes were standardised to nine (LS9), six (LS6) or three (LS3) pups, by day 2 of lactation. After a further 10 d, LS9 and LS6 rats carried more worms than LS3 rats. However, feeding treatments did not affect concentrations of mucosal inflammatory cells. Achieved feed intake did not differ consistently between the treatment groups. However, LS9 and LS6 rats lost weight, whilst LS3 rats gained weight during lactation. The results support the view that resistance to N. brasiliensis is sensitive to changes in nutrient demand, and the improved resistance to N. brasiliensis is likely due to effects of overcoming nutrient scarcity on host immunity.

scholarly journals Neither Primary nor Memory Immunity to Mycobacterium tuberculosis Infection Is Compromised in Mice with Chronic Enteric Helminth Infection

2015 ◽  
Vol 83 (3) ◽  
pp. 1217-1223 ◽  
Author(s):  
Wasiulla Rafi ◽  
Kamlesh Bhatt ◽  
William C. Gause ◽  
Padmini Salgame
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Regulatory Cells ◽  
Helminth Infection ◽  
Treg Cells ◽  
Helminth Species ◽  
Nippostrongylus Brasiliensis ◽  
T Regulatory Cell ◽  
Chronic Infections ◽  
Mycobacterium Tuberculosis Infection ◽  
Content Type

Previously we had reported thatNippostrongylus brasiliensis, a helminth with a lung migratory phase, affected host resistance againstMycobacterium tuberculosisinfection through the induction of alternatively activated (M2) macrophages. Several helminth species do not have an obligatory lung migratory phase but establish chronic infections in the host that include potent immune downregulatory effects, in part mediated through induction of a FoxP3+T regulatory cell (Treg) response. Treg cells exhibit duality in their functions in host defense againstM. tuberculosisinfection since their depletion leads to enhanced priming of T cells in the lymph nodes and attendant improved control ofM. tuberculosisinfection, while their presence in the lung granuloma protects against excessive inflammation.Heligmosomoides polygyrusis a strictly murine enteric nematode that induces a strong FoxP3 Treg response in the host. Therefore, in this study we investigated whether host immunity toM. tuberculosisinfection would be modulated in mice with chronicH. polygyrusinfection. We report that neither primary nor memory immunity conferred byMycobacterium bovisBCG vaccination was affected in mice with chronic enteric helminth infection, despite a systemic increase in FoxP3+T regulatory cells. The findings indicate that anti-M. tuberculosisimmunity is not similarly affected by all helminth species and highlight the need to consider this inequality in human coinfection studies.

scholarly journals Nippostrongylus brasiliensis: Identification of intelectin-1 and -2 as Stat6-dependent genes expressed in lung and intestine during infection

2007 ◽  
Vol 116 (4) ◽  
pp. 458-466 ◽  
Author(s):  
David Voehringer ◽  
Sarah A. Stanley ◽  
Jeffery S. Cox ◽  
Gladys C. Completo ◽  
Todd L. Lowary ◽  
...  
Keyword(s):  
Nippostrongylus Brasiliensis
Sign in / Sign up

Export Citation Format

Share Document