Ion Etching of Dental Tissues in a Scanning Electron Microscope

Nature ◽  
1962 ◽  
Vol 196 (4849) ◽  
pp. 81-82 ◽  
Author(s):  
A. D. G. STEWART ◽  
A. BOYDE
1979 ◽  
Vol 57 (22) ◽  
pp. 2528-2532 ◽  
Author(s):  
Yosio Yukawa ◽  
Shuhei Tanaka

Clubroot galls taken from 40-day-old Brassica rapa L. var. glabra Kitamura, grown in soil infested with Plasmodiophora brassicae Wor. were examined by scanning (SEM) and transmission (TEM) microscopy. SEM materials were prepared by freeze fracturing followed by ion etching. A thin layer enveloped the mass of sporangia which in etched specimens appeared to be embedded in a fibrillar net deposited among the sporangia. Both SEM and TEM micrographs revealed a circular germ pore (ca. 1 μm in diameter) with a thickened plug-like region in the pore orifice. The wall of the mature resting sporangium consisted of three layers W1, W2, and W3.


2009 ◽  
Vol 4 (2) ◽  
pp. 190-195
Author(s):  
Ewa Bałczewska ◽  
Leszek Klimek ◽  
Aleksandra Palatyńska-Ulatowska

AbstractThe aim of the present study is to examine microscopically the surface of dental enamel by using a scanning electron microscope (SEM), using their replicas formed in female patients with diagnosed periodontal diseases and systemic calcium deficiency. Replicas of dental enamel surfaces in patients referred for treatment of periodontal diseases were subjected to microscopic analysis. The replicas, after coating with platinum-palladium alloy, were examined under the scanning electron microscope at magnifications of 15–5000 x. Densitometric examinations of spine (L2 - L4 segment) revealed bone mineral density BMD T-score lower than −2.5 in 5 patients, in the range of −1.5 to −2.5 in 10 patients, and higher than −1.5 in the remaining patients. Non-homogenous images of surfaces in the form of light and dark areas were observed. Light areas corresponded to damaged surfaces of dental tissues. Patients with higher systemic calcium deficiency had areas lighter in color. More of these areas were found in patients with higher systemic calcium deficiency. It can be assumed that the calcium deficit is likely to appear in the selected dental tissues, particularly in the dental enamel.


2011 ◽  
Vol 166 (12) ◽  
pp. 927-934 ◽  
Author(s):  
Nabil El-Faramawy ◽  
Reham Ameen ◽  
Khaled El-Haddad ◽  
Ahmed Maghraby ◽  
Medhat El-Zainy

Detailed descriptions of the microstructure, which includes both histology and ultrastructure, are given for each type of tissue in each of the three species of Australian dipnoan. Most of the tissues have not previously been recorded in this way even for other species within the same genera. The microstructure is interpreted from examination of ground sections in transmitted and polarized light, together with examination of functional, forming and fracture surfaces in the scanning electron microscope. No previous studies of dipnoan dental tissues have been published in which the histology is related to surface morphology as seen in the scanning electron microscope. The histology, microstructure, growth and distribution of buccal denticles are described in Griphognathus whitei and Holodipterus gogoensis , and their morphogenesis and adaptation to function is discussed. It is concluded that the phyletic trend towards macromerism, as shown in chondricthyans and primitive teleostomes, is illustrated in the three species of dipnoan. The continuous layer of dentine of the tooth ridges, lips and covering to the dermal snout is described and found to be similar in the three species and equivalent to the tissue described previously in other dipnoans, sometimes as a form of cosmine. A term not in current usage is suggested for this dentine, namely syndentine. Dentine terminology is reviewed and the relevance to dipnoan dental tissues is discussed with the conclusion that an older term should be reintroduced to eliminate the current anomalies in terminology. Chirodipterus australis is the only species with typical tooth plates. The histology is compared with previous accounts of tooth plates in dipnoans and found to have some similarity with those of Neoceratodus forsteri . The microstructure is reported from examination of the worn functional surfaces and acid-etched functional surfaces with the scanning electron microscope, this information has not previously been reported for dipnoan tooth plates and is presented here as a basis for comparison with other species. New features of cosmine structure are described from scanning electron micrographs of the surfaces and these are related to the probable mode of formation and the involvement of the epithelial cells. The structure is found to compare in some ways with the cosmine of osteolepids. The loss of cosmine and replacement by tubercles in Griphognathus whitei and Holodipterus gogoensis is explained as retention of an ontogenetic potential which is comparable with Ørvig’s theory of cosmine regression in the porolepids. Superpositional growth in the dermal skull bones is described for the first time in dipnoans. It is postulated that the ancestors of dipnoans had superimposed denticles beneath a cosmine covering. The information obtained from the microstructure is used to examine the hypothesis proposed by Miles (1977) in a paper on the phyletic relations within the dipnoans. This confirms that Griphognathus white i has retained a primitive dentition with separate buccal denticles and tooth ridges; Holodipterus gogoensis has retained these features, denticles and lip ridges, together with an advanced feature of tooth cusps which are a divergent specialization phyletically preceding tooth plates; Chirodipterus australis has not retained buccal denticles but has lip ridges on the snout and anterior part of the lower jaw, and the specialized tooth plates. Pleromic dentine is recorded in two of these species and discussed with other observed methods of adaptation to wear.


2019 ◽  
Vol 56 (3) ◽  
pp. 578-582
Author(s):  
Gianina Iovan ◽  
Sorina Solomon ◽  
Simona Stoleriu ◽  
Angela Cristina Ghiorghe ◽  
Irina Nica ◽  
...  

Biodentine is a tricalcium silicate cement used as a dentin replacement in restorative dentistry. The aim of this study was to assess the influence of cavity moisture on the immediate sealing and morphology of the interface between Biodentine and the dental tissues. 20 class II cavities were prepared in extracted teeth. 10 cavities were dried using the air spray before restoring with Biodentine and 10 cavities were preserved moist for the restorative procedure. The teeth were subjected to a microleakage test and then the axial sections were observed under optical microscope and scanning electron microscope. In most situations Biodentine provided an immediate good marginal sealing and a tide contact with adjacent tissues in both occlusal and cervical margins which did not seem to be influenced by the moisture of the dental supporting tissues.


Author(s):  
R. E. Ferrell ◽  
G. G. Paulson

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.


Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.


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