Pyruvic Acid, a Unique Component of an Exocellular Bacterial Polysaccharide

Nature ◽  
1962 ◽  
Vol 194 (4827) ◽  
pp. 478-479 ◽  
Author(s):  
J. H. SLONEKER ◽  
DANUTE G. ORENTAS
Keyword(s):  
Pyruvic Acid ◽  
Bacterial Polysaccharide

EXOCELLULAR BACTERIAL POLYSACCHARIDE FROM XANTHOMONAS CAMPESTRIS NRRL B-1459: PART I. CONSTITUTION

1962 ◽  
Vol 40 (11) ◽  
pp. 2066-2071 ◽  
Author(s):  
J. H. Sloneker ◽  
Allene Jeanes
Keyword(s):  
Acid Hydrolysis ◽  
Pyruvic Acid ◽  
Potassium Salt ◽  
Xanthomonas Campestris ◽  
Glucuronic Acid ◽  
Solution Viscosity ◽  
Bacterial Polysaccharide ◽  
Partial Acid Hydrolysis ◽  
Acid Stable ◽  
End Group

Polysaccharide B-1459 is the first bacterial polysaccharide reported to contain pyruvic acid as a constituent. The polysaccharide, isolated as the potassium salt, was shown to be composed of D-glucose, D-mannose, and D-glucuronic acid, acetic acid, and pyruvic acid in the ratio 2.8:3.0:2.0:1.7:0.51–0.63. One-third of the total mannose was released readily as free mannose by graded acid hydrolysis with only a slow loss of the high solution viscosity. The remainder of the mannose was isolated as the acid-stable aldobiouronic acid 2-O-(β-D-glucopyranosyluronic acid)-D-mannose. Partial acid hydrolysis and preparative paper chromatography afforded two higher oligosaccharides: an aldotriouronic acid composed of glucuronic acid β-linked 1,2 to mannose with glucose as the reducing end group, and what appears to be an aldotetraouronic acid composed of glucuronic acid, mannose, and glucose.

Mg++ - and Ca++-Induced Platelet Aggregation and ADP

1970 ◽  
Vol 24 (03/04) ◽  
pp. 432-437 ◽  
Author(s):  
S Cronberg ◽  
J. P Caen
Keyword(s):  
Platelet Aggregation ◽  
Pyruvate Kinase ◽  
Pyruvic Acid ◽  
Active Principle ◽  
Platelet Suspension ◽  
Edta Plasma ◽  
Phosphoenol Pyruvic Acid

SummaryReports on platelet aggregation after addition of calcium or magnesium to EDTA- PRP or platelet suspensions were confirmed. An aggregating principle was found in the EDTA-plasma and the supernatant of the platelet suspensions. Aggregation by magnesium in a platelet suspension was inhibited by adenosine and phosphoenol- pyruvic acid and pyruvate kinase, which suggested that the active principle was identical with ADP. Degradation of ADP in EDTA plasma was blocked.It thus appears that aggregation induced by calcium or magnesium in EDTA-PRP and platelet suspension was due to accumulation of spontaneously liberated ADP, which was not degraded.

scholarly journals THE RESPIRATORY DECOMPOSITION OF PYRUVIC ACID BY BARLEY

1938 ◽  
Vol 37 (5) ◽  
pp. 455-473 ◽  
Author(s):  
W. O. JAMES ◽  
I. P. NORVAL
Keyword(s):  
Pyruvic Acid
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