Effect of Adrenalectomy on the Activity of Enyzmes of the Urea Cycle in Rat Liver

Nature ◽  
1961 ◽  
Vol 191 (4795) ◽  
pp. 1302-1303 ◽  
Author(s):  
PATRICIA McLEAN
Keyword(s):  
Rat Liver ◽  
Urea Cycle

scholarly journals N-Acetylglutamate in rat liver during foetal development

1984 ◽  
Vol 222 (3) ◽  
pp. 837-838 ◽  
Author(s):  
M Van Dijk ◽  
P Lund
Keyword(s):  
Rat Liver ◽  
Urea Cycle ◽  
Developmental Pattern ◽  
Foetal Development ◽  
Tissue Content ◽  
Foetal Rat ◽  
Foetal Life

N-Acetylglutamate is present in foetal rat liver at 17 days' gestation. The tissue content (approx. 50 nmol/g wet wt.) remains constant during later foetal life. The appearance of N-acetylglutamate does not parallel the developmental pattern of the urea cycle.

scholarly journals Real-time study of the urea cycle using 15N n.m.r. in the isolated perfused rat liver

1992 ◽  
Vol 287 (3) ◽  
pp. 813-820 ◽  
Author(s):  
A Geissler ◽  
K Kanamori ◽  
B D Ross
Keyword(s):  
Rat Liver ◽  
Urea Cycle ◽  
Isolated Perfused Rat Liver ◽  
15N Recovery ◽  
Urea Synthesis ◽  
Perfused Liver ◽  
Biochemical Assays ◽  
Low Concentrations ◽  
15N Urea ◽  
Rate Limiting

1. Isolated rat liver was perfused with 10 mM-15NH4Cl, 5 mM-lactate and 1 mM-ornithine, or with 3 mM-[15N]alanine and 1 mM-ornithine, in haemoglobin-free medium. The liver was physiologically stable for over 3 h and synthesized urea at the rate of 1.15 mumol.min-1.g of liver-1 (15NH4(+)-perfused) or 0.41 mumol.min-1.g-1 ([15N]alanine-perfused). 2. The perfused liver was continuously monitored by 15N n.m.r. spectroscopy at 20.27 MHz for 15N. Well-resolved 15N resonances of precursors and intermediates of the urea cycle, present at tissue concentrations of 0.2-3.0 mumol/g, were observed from the intact liver in 5-40 min of acquisition. Key metabolites in liver extract and the final perfusion medium were analysed by n.m.r. and by biochemical assays to determine fractional 15N enrichment and the total 15N recovery. 3. In 15NH4(+)-perfused liver (n = 6), 15N incorporation into glutamate and alanine (1.0-1.3 mumol/g), as well as progressive formation of [15N2]urea, was observed during the first 2 h of perfusion. In the second and third hour, hepatic concentrations of [omega-15N]citrulline and [omega, omega'-15N]argininosuccinate increased to n.m.r.-detectable levels (0.3-0.9 mumol/g). The [15N]aspartate pool was large in the absence of added ornithine, but on its addition was rapidly incorporated into argininosuccinate (n = 3). 4. In [15N]alanine-perfused liver, major metabolites were [15N]glutamate, [gamma-15N]glutamine and [15N]urea. Urea-cycle intermediates were undetectable. 5. The results suggest that, in intact liver provided with excess ammonia, low concentrations of cytosolic argininosuccinate synthetase and argininosuccinate lyase limited the rate of metabolite flux in the urea cycle. By contrast, in alanine-perfused liver at a physiological rate of urea synthesis, mitochondrial carbamoylphosphate synthetase was rate-limiting. 6. The potential utility of 15N n.m.r. for study of metabolite channelling through urea-cycle enzymes in intact liver is discussed.

Modulation of mRNA Levels for Urea Cycle Enzymes in Rat Liver by Diet and Hormones

1986 ◽  
Vol 478 (1 Metabolic Reg) ◽  
pp. 292-294 ◽  
Author(s):  
SIDNEY M. MORRIS ◽  
CAROLE L. MONCMAN ◽  
WILLIAM E. O'BRIEN
Keyword(s):  
Rat Liver ◽  
Urea Cycle ◽  
Mrna Levels ◽  
Urea Cycle Enzymes

Studies on Urea Cycle Enzymes in Rat Liver during Acute Uraemia

1975 ◽  
Vol 5 (1) ◽  
pp. 15-20 ◽  
Author(s):  
G. Hoppe-Seyler ◽  
K. P. Maier ◽  
P. Schollmeyer ◽  
J. Frohlich ◽  
H. Talke ◽  
...  
Keyword(s):  
Rat Liver ◽  
Urea Cycle ◽  
Urea Cycle Enzymes

Hormonal Regulation of Four Urea Cycle Enzymes in Postnatal Rat Liver in Organ Culture

Enzyme ◽  
1985 ◽  
Vol 34 (4) ◽  
pp. 186-195 ◽  
Author(s):  
Adele Hart ◽  
John B. Balinsky
Keyword(s):  
Organ Culture ◽  
Rat Liver ◽  
Hormonal Regulation ◽  
Urea Cycle ◽  
Urea Cycle Enzymes

Regulation of mRNA levels for five urea cycle enzymes in rat liver by diet, cyclic AMP, and glucocorticoids

1987 ◽  
Vol 256 (1) ◽  
pp. 343-353 ◽  
Author(s):  
Sidney M. Morris ◽  
Carole L. Moncman ◽  
Katherine D. Rand ◽  
George J. Dizikes ◽  
Stephen D. Cederbaum ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Rat Liver ◽  
Urea Cycle ◽  
Mrna Levels ◽  
Urea Cycle Enzymes
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