Regulation of Genes for Inducible Nitric Oxide Synthase and Urea Cycle Enzymes in Rat Liver in Endotoxin Shock

2000 ◽  
Vol 268 (1) ◽  
pp. 221-224 ◽  
Author(s):  
Shoko Tabuchi ◽  
Tomomi Gotoh ◽  
Kei Miyanaka ◽  
Kimio Tomita ◽  
Masataka Mori
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Liver ◽  
Inducible Nitric Oxide Synthase ◽  
Urea Cycle ◽  
Endotoxin Shock ◽  
Oxide Synthase ◽  
Urea Cycle Enzymes ◽  
Inducible Nitric Oxide

LPS-induced downregulation of MRP2 and BSEP in human liver is due to a posttranscriptional process

2004 ◽  
Vol 287 (5) ◽  
pp. G1008-G1016 ◽  
Author(s):  
Marieke G. L. Elferink ◽  
Peter Olinga ◽  
Annelies L. Draaisma ◽  
Marjolijn T. Merema ◽  
Klaas Nico Faber ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Liver ◽  
Inducible Nitric Oxide Synthase ◽  
Human Liver ◽  
Mrna Levels ◽  
Liver Slices ◽  
Oxide Synthase ◽  
Human Liver Slices ◽  
Inducible Nitric Oxide

Endotoxin-induced cholestasis in rodents is caused by hepatic downregulation of transporters, including the basolateral Na+-dependent taurocholate transporter (ntcp) and the canalicular bile salt export pump (bsep) and multidrug resistance-associated protein 2 (mrp2). Details about the regulation of the human transporter proteins during this process are lacking. We used precision-cut human and rat liver slices to study the regulation of transporter expression during LPS-induced cholestasis. We investigated the effect of LPS on nitrate/nitrite and cytokine production in relation to the expression of inducible nitric oxide synthase, NTCP, BSEP, and MRP2 both at the level of mRNA with RT-PCR and protein using immunofluorescence microscopy. In liver slices from both species, LPS-induced expression of inducible nitric oxide synthase was detected within 1–3 h and remained increased over 24 h. In rat liver slices, this was accompanied by a significant decrease of rat ntcp and mrp2 mRNA levels, whereas bsep levels were not affected. These results are in line with previous in vivo studies and validate our liver slice technique. In LPS-treated human liver slices, NTCP mRNA was downregulated and showed an inverse correlation with the amounts of TNF-α and Il-1β produced. In contrast, MRP2 and BSEP mRNA levels were not affected under these conditions. However, after 24-h LPS challenge, both proteins were virtually absent in human liver slices, whereas marker proteins remained detectable. In conclusion, we show that posttranscriptional mechanisms play a more prominent role in LPS-induced regulation of human MRP2 and BSEP compared with the rat transporter proteins.

Chronic iron overload enhances inducible nitric oxide synthase expression in rat liver

Nitric Oxide ◽  
2005 ◽  
Vol 13 (1) ◽  
pp. 54-61 ◽  
Author(s):  
Pamela Cornejo ◽  
Patricia Varela ◽  
Luis A. Videla ◽  
Virginia Fernández
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Iron Overload ◽  
Rat Liver ◽  
Inducible Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

S-Adenosylmethionine modulates inducible nitric oxide synthase gene expression in rat liver and isolated hepatocytes

2001 ◽  
Vol 35 (6) ◽  
pp. 692-699 ◽  
Author(s):  
Pedro Lorenzo Majano ◽  
Carmelo Garcı́a-Monzón ◽  
Elena Ruiz Garcı́a-Trevijano ◽  
Fernando José Corrales ◽  
Javier Cámara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Liver ◽  
Inducible Nitric Oxide Synthase ◽  
Isolated Hepatocytes ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide
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