Glutamine Metabolism of Normal and Malignant Cells cultivated in Synthetic Media

Nature ◽  
1959 ◽  
Vol 183 (4669) ◽  
pp. 1201-1202 ◽  
Author(s):  
ARTHUR E. PASIEKA ◽  
JOSEPH F. MORGAN
Keyword(s):  
Glutamine Metabolism ◽  
Malignant Cells ◽  
Synthetic Media

CARBOHYDRATE UTILIZATION BY CHICK EMBRYONIC HEART CULTURES

1960 ◽  
Vol 38 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Joseph F. Morgan ◽  
Helen J. Morton
Keyword(s):  
Embryonic Heart ◽  
Serum Enzymes ◽  
Cell Multiplication ◽  
Complete Medium ◽  
Malignant Cells ◽  
Carbohydrate Utilization ◽  
Physiological Measurement ◽  
Normal Tissues ◽  
Heart Cultures ◽  
Synthetic Media

Freshly explanted chick embryonic heart fragments were cultivated in completely synthetic media. Survival of such cultures in the complete medium, containing glucose, was established at approximately 35 to 40 days, while in the absence of carbohydrate the cultures died within 3 to 5 days. Survival was considered to be a more physiological measurement than rapid cell multiplication for normal tissues and was adopted as the criterion for all experiments reported. Fifty-two compounds were tested for their ability to replace glucose, as the sole carbohydrate, in this system. Of these, seven (mannose, fructose, galactose, β-glucose, maltose, glucose-1-phosphate, and glucose-6-phosphate) replaced glucose completely. Five others (sorbitol, alpha-methyl-D-glucoside, turanose, dextrin, and fructose-6-phosphate) were partially active. The remainder were negative. Comparison is made of the present results with those obtained by other workers using malignant cells in the presence of serum–enzymes. The present results suggest that the ability to replace glucose decreases progressively as compounds down the Embden–Meyerhof pathway are tested.

CARBOHYDRATE UTILIZATION BY CHICK EMBRYONIC HEART CULTURES

1960 ◽  
Vol 38 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Joseph F. Morgan ◽  
Helen J. Morton
Keyword(s):  
Embryonic Heart ◽  
Serum Enzymes ◽  
Cell Multiplication ◽  
Complete Medium ◽  
Malignant Cells ◽  
Carbohydrate Utilization ◽  
Physiological Measurement ◽  
Normal Tissues ◽  
Heart Cultures ◽  
Synthetic Media

Freshly explanted chick embryonic heart fragments were cultivated in completely synthetic media. Survival of such cultures in the complete medium, containing glucose, was established at approximately 35 to 40 days, while in the absence of carbohydrate the cultures died within 3 to 5 days. Survival was considered to be a more physiological measurement than rapid cell multiplication for normal tissues and was adopted as the criterion for all experiments reported. Fifty-two compounds were tested for their ability to replace glucose, as the sole carbohydrate, in this system. Of these, seven (mannose, fructose, galactose, β-glucose, maltose, glucose-1-phosphate, and glucose-6-phosphate) replaced glucose completely. Five others (sorbitol, alpha-methyl-D-glucoside, turanose, dextrin, and fructose-6-phosphate) were partially active. The remainder were negative. Comparison is made of the present results with those obtained by other workers using malignant cells in the presence of serum–enzymes. The present results suggest that the ability to replace glucose decreases progressively as compounds down the Embden–Meyerhof pathway are tested.

Nuclear coiled bodies and the nucleolus

1994 ◽  
Vol 52 ◽  
pp. 20-21
Author(s):  
K. Brasch ◽  
J. Williams ◽  
D. Gallo ◽  
T. Lee ◽  
R. L. Ochs
Keyword(s):  
Mouse Liver ◽  
Nuclear Body ◽  
Nuclear Bodies ◽  
Model Systems ◽  
Coiled Body ◽  
Rrna Processing ◽  
Malignant Cells ◽  
Sm Proteins ◽  
Coiled Bodies ◽  
Unique Protein

Though first described in 1903 by Ramon-y-Cajal as silver-staining “accessory bodies” to nucleoli, nuclear bodies were subsequently rediscovered by electron microscopy about 30 years ago. Nuclear bodies are ubiquitous, but seem most abundant in hyperactive and malignant cells. The best studied type of nuclear body is the coiled body (CB), so termed due to characteristic morphology and content of a unique protein, p80-coilin (Fig.1). While no specific functions have as yet been assigned to CBs, they contain spliceosome snRNAs and proteins, and also the nucleolar protein fibrillarin. In addition, there is mounting evidence that CBs arise from or are generated near the nucleolus and then migrate into the nucleoplasm. This suggests that as yet undefined links may exist, between nucleolar pre-rRNA processing events and the spliceosome-associated Sm proteins in CBs.We are examining CB and nucleolar changes in three diverse model systems: (1) estrogen stimulated chick liver, (2) normal and neoplastic cells, and (3) polyploid mouse liver.

CLINICAL SYMPTOMS, BIOCHEMISTRY AND CYTOLOGY OF PLEURAL, PERITONEAL EFFUSIONS

2016 ◽  
pp. 66-71
Author(s):  
Van Mao Nguyen ◽  
Huyen Quynh Trang Pham
Keyword(s):  
Pleural Effusion ◽  
Clinical Symptoms ◽  
Cross Sectional Study ◽  
Malignant Cells ◽  
Sectional Study ◽  
Pleural Effusions ◽  
Cross Sectional ◽  
Breath Sounds ◽  
Peritoneal Effusion ◽  
Neutrophil Leukocytes

Background: The cytology and the support of clinical symptoms, biochemistry for diagnosis of the cases of effusions are very important. Objectives: - To describe some of clinical symptoms and biochemistry of effusions. - To compare the results between cytology and biochemistry by the causes of pleural, peritoneal fluids. Material & Method: A cross-sectional study to describe all of 47 patients with pleural, peritoneal effusions examinated by cytology in the Hospital of Hue University of Medicine and Pharmacy from April 2013 to January 2014. Results: In 47 cases with effusions, pleural effusion accounting for 55.32%, following peritoneal effusions 29.79% and 14.89% with both of them. The most common symptoms in patients with pleural effusions were diminished or absent tactile fremitus, dull percussion, diminished or absent breath sounds (100%), in patients with peritoneal effusions was ascites (95.24%). 100% cases with pleural effusions, 50% cases with peritoneal effusions and 80% cases with pleural and peritoneal effusions were exudates. The percentage of malignant cells in patients with pleural effusions was 26.92%, in peritoneal effusions was 28.57%, in pleural and peritoneal effusions was 42.86%. The percentage of detecting the malignant cells in patients with suspected cancer in the first test was 57.14%, in the second was 9.53% and 33.33% undetectable. Most of cases which had malignant cells and inflammatory were exudates, all of the cases which had a few cells were transudates. Besides, 7.5% cases which had high neutrophil leukocytes were transudates. Conclusion: Cytology should be carry out adding to the clinical examinations and biochemistry tests to have an exact diagnosis, especially for the malignant ones. For the case with suspected cancer, we should repeat cytology test one more time to increase the ability to detect malignant cells. Key words: Effusion, pleural effusion, peritoneal effusion, cytology, biochemistry

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