Small-Scale Preparation of Glyoxylic Acid labelled with Carbon-14

Nature ◽  
1958 ◽  
Vol 181 (4605) ◽  
pp. 335-336 ◽  
Author(s):  
R. M. KRUPKA ◽  
G. H. N. TOWERS
1952 ◽  
Vol 74 (9) ◽  
pp. 2418-2419 ◽  
Author(s):  
E. C. Jorgensen ◽  
J. A. Bassham ◽  
M. Calvin ◽  
B. M. Tolbert

1972 ◽  
Vol 126 (4) ◽  
pp. 781-790 ◽  
Author(s):  
N. W. Penn ◽  
R. Suwalski ◽  
C. O'Riley ◽  
K. Bojanowski ◽  
R. Yura

A method is given for small-scale preparation of DNA from 1.0–1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.


2009 ◽  
Vol 20 (2) ◽  
pp. 139-142 ◽  
Author(s):  
G. Qi ◽  
J. T. Li ◽  
Q. P. Ruan ◽  
J. Yang ◽  
Z. X. Su

2014 ◽  
Vol 69 (7) ◽  
pp. 326-330 ◽  
Author(s):  
Paul I. Dron ◽  
Walter E. Klein ◽  
Josef Michl

A convenient procedure for the preparation of p-carborane by pyrolysis of o-carborane on a scale of 300 - 350 mg of > 99:5% pure product per day is described.


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