An optimised, small-scale preparation of high-quality RNA from dry seeds ofDavidia involucrata

2009 ◽  
Vol 20 (2) ◽  
pp. 139-142 ◽  
Author(s):  
G. Qi ◽  
J. T. Li ◽  
Q. P. Ruan ◽  
J. Yang ◽  
Z. X. Su
Keyword(s):  
Small Scale ◽  
High Quality ◽  
Scale Preparation

scholarly journals From Coffee to Biodiesel—Deep Eutectic Solvents for Feedstock and Biodiesel Purification

Separations ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 22 ◽  
Author(s):  
Aleksandra Sander ◽  
Ana Petračić ◽  
Jelena Parlov Vuković ◽  
Lana Husinec
Keyword(s):  
Potassium Carbonate ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Acid Number ◽  
Biodiesel Production ◽  
Small Scale ◽  
Total Acid ◽  
High Quality ◽  
Purification Method ◽  
Coffee Grounds

Over three billion cups of coffee are consumed daily, making waste coffee grounds readily available throughout the world. Containing approximately 10–15 wt% of oil, they have great potential for biodiesel production. The goal of this work was to produce high quality biodiesel from waste coffee grounds. One fresh and four different types of waste coffee grounds were collected. Oil was extracted by the Soxhlet method with n-hexane and then purified via extractive deacidification with a potassium carbonate-based deep eutectic solvent. Biodiesels were synthesized by means of alkali catalyzed transesterification at different catalyst:methanol:oil mass ratios and reaction times. Impurities present in crude biodiesels were extracted with a choline chloride-based deep eutectic solvent. All batch extraction experiments were performed at room conditions in a small scale extractor. Optimal conditions for synthesis and purification were defined in order to assure high quality of the produced biodiesel. Additionally, continuous column extraction with the choline chloride-based solvent was tested as a purification method for crude biodiesel. Stabilization time and optimal biodiesel to solvent mass ratio were determined. The potassium carbonate-based solvent efficiently reduced the total acid number of the feedstock (deacidification efficiency ranged from 86.18 to 94.15%), while the one based on choline chloride removed free glycerol and glycerides from crude biodiesels. After continuous purification, the purified biodiesel was of excellent quality with glycerol and glyceride contents below the EN 14214 limit.

scholarly journals The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid

1972 ◽  
Vol 126 (4) ◽  
pp. 781-790 ◽  
Author(s):  
N. W. Penn ◽  
R. Suwalski ◽  
C. O'Riley ◽  
K. Bojanowski ◽  
R. Yura
Keyword(s):  
Deoxyribonucleic Acid ◽  
Phosphorus Content ◽  
Alkaline Solutions ◽  
Small Scale ◽  
Rat Tissues ◽  
Adult Rat ◽  
Frog Brain ◽  
Rat Brain And Liver ◽  
Scale Preparation ◽  
Rat Tissue

A method is given for small-scale preparation of DNA from 1.0–1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.

Convenient Small-Scale Preparation of p-Carborane by Pyrolysis of o-Carborane

2014 ◽  
Vol 69 (7) ◽  
pp. 326-330 ◽  
Author(s):  
Paul I. Dron ◽  
Walter E. Klein ◽  
Josef Michl
Keyword(s):  
Small Scale ◽  
Convenient Procedure ◽  
Pure Product ◽  
Scale Preparation

A convenient procedure for the preparation of p-carborane by pyrolysis of o-carborane on a scale of 300 - 350 mg of > 99:5% pure product per day is described.

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