Changes of Organic Phosphate Esters during Glycolysis in Blood followed by a Paper Chromatography Method

Nature ◽  
1957 ◽  
Vol 180 (4598) ◽  
pp. 1351-1352 ◽  
Author(s):  
L. MACHO
Keyword(s):  
Paper Chromatography ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Chromatography Method

Paper Chromatography of Phosphate Esters

Nature ◽  
1953 ◽  
Vol 171 (4358) ◽  
pp. 838-839 ◽  
Author(s):  
E. FLETCHER ◽  
F. H. MALPRESS
Keyword(s):  
Paper Chromatography ◽  
Phosphate Esters

Diamagnetic Susceptibilities of Various Inorganic Phosphates and Organic Phosphate Esters

1993 ◽  
Vol 66 (2) ◽  
pp. 371-373 ◽  
Author(s):  
Fujio Takahashi ◽  
Yasuzo Sakai ◽  
Toshihito Yoshida
Keyword(s):  
Organic Phosphate ◽  
Phosphate Esters ◽  
Inorganic Phosphates

scholarly journals CHLORIDE-DEPENDENT ORGANIC PHOSPHATE SENSITIVITY OF THE OXYGENATION REACTION IN CROCODILIAN HEMOGLOBINS

1994 ◽  
Vol 192 (1) ◽  
pp. 1-11 ◽  
Author(s):  
R Weber ◽  
F White
Keyword(s):  
Molecular Mechanisms ◽  
Feedback Regulation ◽  
Organic Phosphate ◽  
Alligator Mississippiensis ◽  
Phosphate Esters ◽  
Caiman Crocodilus ◽  
Allosteric Interaction ◽  
Allosteric Effector ◽  
Intensive Exercise ◽  
O2 Supply

We show that crocodilian hemoglobins (Hbs), which previously have been considered to be model pigments lacking allosteric interaction with organic phosphate esters, do exhibit oxylabile ATP and 2,3-diphosphoglycerate (DPG) binding that decreases O2 affinity and increases pH sensitivity (Bohr effect), in the absence of Cl- and at the low Cl- concentrations that may occur in crocodilian plasma during 'post-ingestive alkaline tides'. Hbs from different species vary in their phosphate sensitivities. In Alligator mississippiensis Hb, O2 affinity shows greater ATP than DPG sensitivity at low [cofactor]/[Hb] ratios. In Paleosuchus palpebrosus Hb, where even a high Cl- concentration (0.1 mol l-1) does not completely suppress the phosphate effects, the opposite is true, whereas both ATP and DPG exert similar effects in Caiman crocodilus Hb. Lactate, at concentrations that may occur after intensive exercise, similarly depresses Hb O2-affinity, indicating an O2 demand/O2 supply feedback regulation. Curiously, inositol hexaphosphate (IHP), a potent allosteric effector in other vertebrate Hbs, has no effect on A. mississippiensis and P. palpebrosus Hb and only small effects on C. crocodilus Hb, presumably because of steric hindrance at the binding site. The molecular mechanisms underlying the observations (particularly the implication of ss82-Lys, i.e. the lysine residue at position 82 of the ss-chains that binds phosphate and Cl-) are considered. Their physiological significance requires further study.

STUDIES ON THE PRESERVATION OF BLOOD: VII. THE INFLUENCE OF INOSINE ON THE METABOLIC BEHAVIOR OF THE ERYTHROCYTE DURING THE PRESERVATION OF BLOOD IN THE COLD

1959 ◽  
Vol 37 (1) ◽  
pp. 69-79
Author(s):  
D. Rubinstein ◽  
S. Kashket ◽  
Rhoda Blostein ◽  
O. F. Denstedt
Keyword(s):  
Cold Storage ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Blood Samples ◽  
The Third ◽  
Duration Of Storage ◽  
The Difference ◽  
Blood Specimens ◽  
Metabolic Behavior ◽  
High Degree

Inosine, like other purine nucleosides when added to preserved blood specimens, induces a resynthesis of organic phosphate esters in the erythrocytes. Apart from the difference in rates of reaction, the metabolic reconstitution of the cells is the same at 37° and 4 °C. The reconstitution of the esters occurs rapidly even at 4 °C, and the higher the concentration of inosine added, the more prolonged is the maintenance of the esters. When inosine was added repeatedly to blood samples during storage, a phase of synthesis was induced with each addition of nucleoside. The capacity of the erythrocytes to resynthesize 2,3-diphosphoglyceric acid (DPG) remained normal regardless of the storage age of the sample but the capacity to replenish ATP decreased with the duration of storage. Addition of inosine at the end of the third week of cold storage can effect a high degree of restoration of metabolic viability in the cells at 4 °C within 24 hours.

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