Diamagnetic Susceptibilities of Various Inorganic Phosphates and Organic Phosphate Esters

1993 ◽  
Vol 66 (2) ◽  
pp. 371-373 ◽  
Author(s):  
Fujio Takahashi ◽  
Yasuzo Sakai ◽  
Toshihito Yoshida
Keyword(s):  
Organic Phosphate ◽  
Phosphate Esters ◽  
Inorganic Phosphates

Selective retention of organic phosphate esters and phosphonates on aluminium oxide

1986 ◽  
Vol 6 (5) ◽  
pp. 477-483 ◽  
Author(s):  
M-A. Coletti-Previero ◽  
M. Pugnière ◽  
H. Mattras ◽  
J. C. Nicolas ◽  
A. Previero
Keyword(s):  
Aqueous Solution ◽  
Aluminium Oxide ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Phosphonic Acids ◽  
Experimental Conditions ◽  
Phosphate Compound ◽  
Phosphorylated Compounds ◽  
Selective Retention ◽  
Inorganic Phosphates

Compounds containing the −PO3H2 function, such as monoesters of phosphoric acid and phosphonic acids, specifically bind to aluminium oxide in aqueous solution under experimental conditions where non-phosphorylated compounds are completely desorbed. The bound organic phosphate can be specifically displaced by aqueous solution of inorganic phosphates thus allowing their separation or detection by a technique similar to that of affinity chromatography. The consequences of this finding for phosphate compound biochemistry are discussed.

Role of Organic Phosphate in Mineralization of Bone in vitro

1981 ◽  
Vol 60 (C) ◽  
pp. 1586-1586 ◽  
Author(s):  
H. C. Tenenbaum
Keyword(s):  
Defined Medium ◽  
Organic Phosphate ◽  
Limiting Factor ◽  
Serum Free ◽  
Osteogenic Cells ◽  
Inorganic Phosphates

Calvarial periostea were dissected from 17-day-old embryonic chicks and folded with the osteogenic cells in apposition. The folded explains were cultured for up to six d on serum and plasma clots or in serum-free hormone-supplemented completely-defined medium. Osteoid consistently formed in such cultures in both types of media, and this osteoid mineralized when appropriate levels of β-glycerophosphate were added to each type of medium. The data presented suggest that the levels of organic phosphate might be more important than inorganic phosphates as a limiting factor in the initiation of mineralization of bone in vitro.

scholarly journals CHLORIDE-DEPENDENT ORGANIC PHOSPHATE SENSITIVITY OF THE OXYGENATION REACTION IN CROCODILIAN HEMOGLOBINS

1994 ◽  
Vol 192 (1) ◽  
pp. 1-11 ◽  
Author(s):  
R Weber ◽  
F White
Keyword(s):  
Molecular Mechanisms ◽  
Feedback Regulation ◽  
Organic Phosphate ◽  
Alligator Mississippiensis ◽  
Phosphate Esters ◽  
Caiman Crocodilus ◽  
Allosteric Interaction ◽  
Allosteric Effector ◽  
Intensive Exercise ◽  
O2 Supply

We show that crocodilian hemoglobins (Hbs), which previously have been considered to be model pigments lacking allosteric interaction with organic phosphate esters, do exhibit oxylabile ATP and 2,3-diphosphoglycerate (DPG) binding that decreases O2 affinity and increases pH sensitivity (Bohr effect), in the absence of Cl- and at the low Cl- concentrations that may occur in crocodilian plasma during 'post-ingestive alkaline tides'. Hbs from different species vary in their phosphate sensitivities. In Alligator mississippiensis Hb, O2 affinity shows greater ATP than DPG sensitivity at low [cofactor]/[Hb] ratios. In Paleosuchus palpebrosus Hb, where even a high Cl- concentration (0.1 mol l-1) does not completely suppress the phosphate effects, the opposite is true, whereas both ATP and DPG exert similar effects in Caiman crocodilus Hb. Lactate, at concentrations that may occur after intensive exercise, similarly depresses Hb O2-affinity, indicating an O2 demand/O2 supply feedback regulation. Curiously, inositol hexaphosphate (IHP), a potent allosteric effector in other vertebrate Hbs, has no effect on A. mississippiensis and P. palpebrosus Hb and only small effects on C. crocodilus Hb, presumably because of steric hindrance at the binding site. The molecular mechanisms underlying the observations (particularly the implication of ss82-Lys, i.e. the lysine residue at position 82 of the ss-chains that binds phosphate and Cl-) are considered. Their physiological significance requires further study.

STUDIES ON THE PRESERVATION OF BLOOD: VII. THE INFLUENCE OF INOSINE ON THE METABOLIC BEHAVIOR OF THE ERYTHROCYTE DURING THE PRESERVATION OF BLOOD IN THE COLD

1959 ◽  
Vol 37 (1) ◽  
pp. 69-79
Author(s):  
D. Rubinstein ◽  
S. Kashket ◽  
Rhoda Blostein ◽  
O. F. Denstedt
Keyword(s):  
Cold Storage ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Blood Samples ◽  
The Third ◽  
Duration Of Storage ◽  
The Difference ◽  
Blood Specimens ◽  
Metabolic Behavior ◽  
High Degree

Inosine, like other purine nucleosides when added to preserved blood specimens, induces a resynthesis of organic phosphate esters in the erythrocytes. Apart from the difference in rates of reaction, the metabolic reconstitution of the cells is the same at 37° and 4 °C. The reconstitution of the esters occurs rapidly even at 4 °C, and the higher the concentration of inosine added, the more prolonged is the maintenance of the esters. When inosine was added repeatedly to blood samples during storage, a phase of synthesis was induced with each addition of nucleoside. The capacity of the erythrocytes to resynthesize 2,3-diphosphoglyceric acid (DPG) remained normal regardless of the storage age of the sample but the capacity to replenish ATP decreased with the duration of storage. Addition of inosine at the end of the third week of cold storage can effect a high degree of restoration of metabolic viability in the cells at 4 °C within 24 hours.

A NEW SPECIFIC ANTIDOTE FOR ORGANIC PHOSPHATE ESTER POISONING

PEDIATRICS ◽  
1961 ◽  
Vol 28 (6) ◽  
pp. 950-955
Author(s):  
William T. Read ◽  
Mollie A. Combes
Keyword(s):  
Organic Phosphate ◽  
Phosphate Esters ◽  
Toxic Effects ◽  
Phosphate Ester ◽  
Accidental Ingestion

As the organic phosphate esters become more widely used, particularly in the home, intoxication from accidental ingestion will no doubt increase. For this reason a discussion of the toxic effects and treatment is presented. The signs, symptoms and treatment are summarized in the Tables.

scholarly journals NMR spectroscopic study of organic phosphate esters coprecipitated with calcite

2016 ◽  
Vol 183 ◽  
pp. 46-62 ◽  
Author(s):  
Brian L. Phillips ◽  
Zelong Zhang ◽  
Laura Kubista ◽  
Silvia Frisia ◽  
Andrea Borsato
Keyword(s):  
Spectroscopic Study ◽  
Organic Phosphate ◽  
Phosphate Esters

Photochemical behavior of organic phosphate esters in aqueous solutions irradiated with a mercury lamp

1992 ◽  
Vol 49 (3) ◽  
pp. 368-374 ◽  
Author(s):  
Seiichi Ishikawa ◽  
Yutaka Uchimura ◽  
Kenzo Baba ◽  
Yoshio Eguchi ◽  
Kozo Kido
Keyword(s):  
Aqueous Solutions ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Mercury Lamp ◽  
Photochemical Behavior

THE METABOLISM OF THE ERYTHROCYTE: XIV. METABOLISM OF NUCLEOSIDES BY THE ERYTHROCYTE

1956 ◽  
Vol 34 (5) ◽  
pp. 927-937 ◽  
Author(s):  
David Rubinstein ◽  
Orville F. Denstedt
Keyword(s):  
Blood Cell ◽  
Experimental Evidence ◽  
Adenosine Deaminase ◽  
Red Blood Cell ◽  
Metabolic Pathway ◽  
Pentose Phosphate ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Red Cell ◽  
Rate Limiting

Adenosine, added to blood, undergoes deamination in the red cells with the formation of inosine. The latter undergoes phosphorolysis to yield hypoxanthine and presumably ribose-1-phosphate. The pentose phosphate is metabolized to lactate yielding ATP in the process. The adenosine deaminase and the purine riboside phosphorylase of the red cell occur in the stroma-free hemolyzate. No adenine is formed from adenosine in the blood, nor is adenine metabolized by the erythrocyte. Xanthosine, guanosine, and inosine, added to blood, follow the same metabolic pathway as adenosine and their metabolism brings about a comparable degree of resynthesis of ATP and other organic phosphate esters and formation of lactate. The experimental evidence indicates that the phosphorolysis of the nucleoside is not the rate-limiting reaction in the metabolism of these compounds in the red blood cell.

THE METABOLISM OF THE ERYTHROCYTE: XIV. METABOLISM OF NUCLEOSIDES BY THE ERYTHROCYTE

1956 ◽  
Vol 34 (1) ◽  
pp. 927-937 ◽  
Author(s):  
David Rubinstein ◽  
Orville F. Denstedt
Keyword(s):  
Blood Cell ◽  
Experimental Evidence ◽  
Adenosine Deaminase ◽  
Red Blood Cell ◽  
Metabolic Pathway ◽  
Pentose Phosphate ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Red Cell ◽  
Rate Limiting

Adenosine, added to blood, undergoes deamination in the red cells with the formation of inosine. The latter undergoes phosphorolysis to yield hypoxanthine and presumably ribose-1-phosphate. The pentose phosphate is metabolized to lactate yielding ATP in the process. The adenosine deaminase and the purine riboside phosphorylase of the red cell occur in the stroma-free hemolyzate. No adenine is formed from adenosine in the blood, nor is adenine metabolized by the erythrocyte. Xanthosine, guanosine, and inosine, added to blood, follow the same metabolic pathway as adenosine and their metabolism brings about a comparable degree of resynthesis of ATP and other organic phosphate esters and formation of lactate. The experimental evidence indicates that the phosphorolysis of the nucleoside is not the rate-limiting reaction in the metabolism of these compounds in the red blood cell.

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