Occurrence of Periodic Acid–Schiff Positive Material in the Pituitary of the Parrot Fish, Pseudoscarus guacamaia

Nature ◽  
1957 ◽  
Vol 180 (4594) ◽  
pp. 1055-1055 ◽  
Author(s):  
A. J. MATTY
Keyword(s):  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Positive Material ◽  
Parrot Fish

scholarly journals Neuropathology of SCA34 showing widespread oligodendroglial pathology with vacuolar white matter degeneration: a case study

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Kokoro Ozaki ◽  
Takashi Irioka ◽  
Toshiki Uchihara ◽  
Akane Yamada ◽  
Ayako Nakamura ◽  
...  
Keyword(s):  
White Matter ◽  
Spinocerebellar Ataxia ◽  
Periodic Acid ◽  
Neuronal Loss ◽  
Spinocerebellar Ataxia Type ◽  
Gene Encoding ◽  
Periodic Acid Schiff ◽  
Positive Material ◽  
Glial Fibrillary Tangles ◽  
Macroscopic Findings

AbstractSpinocerebellar ataxia type 34 (SCA34) is an autosomal dominant inherited ataxia due to mutations in ELOVL4, which encodes one of the very long-chain fatty acid elongases. SCA38, another spinocerebellar ataxia, is caused by mutations in ELOVL5, a gene encoding another elongase. However, there have been no previous studies describing the neuropathology of either SCA34 or 38. This report describes the neuropathological findings of an 83-year-old man with SCA34 carrying a pathological ELOVL4 mutation (NM_022726, c.736T>G, p.W246G). Macroscopic findings include atrophies in the pontine base, cerebellum, and cerebral cortices. Microscopically, marked neuronal and pontocerebellar fiber loss was observed in the pontine base. In addition, in the pontine base, accumulation of CD68-positive macrophages laden with periodic acid-Schiff (PAS)-positive material was observed. Many vacuolar lesions were found in the white matter of the cerebral hemispheres and, to a lesser extent, in the brainstem and spinal cord white matter. Immunohistological examination and ultrastructural observations with an electron microscope suggest that these vacuolar lesions are remnants of degenerated oligodendrocytes. Electron microscopy also revealed myelin sheath destruction. Unexpectedly, aggregation of the four-repeat tau was observed in a spatial pattern reminiscent of progressive supranuclear palsy. The tau lesions included glial fibrillary tangles resembling tuft-shaped astrocytes and neurofibrillary tangles and pretangles. This is the first report to illustrate that a heterozygous missense mutation in ELOVL4 leads to neuronal loss accompanied by macrophages laden with PAS-positive material in the pontine base and oligodendroglial degeneration leading to widespread vacuoles in the white matter in SCA34.

Ovary of the southern hairy-nosed wombat (Lasiorhinus latifrons): its divergent structural organisation

2019 ◽  
Vol 31 (9) ◽  
pp. 1457
Author(s):  
William G. Breed ◽  
Eleanor J. Peirce ◽  
Chris M. Leigh
Keyword(s):  
Zona Pellucida ◽  
Cycle Length ◽  
Periodic Acid ◽  
Oestrous Cycle ◽  
Interstitial Tissue ◽  
Cortical Region ◽  
Periodic Acid Schiff ◽  
Structural Organisation ◽  
Positive Material ◽  
Theca Interna

The organisation of the ovarian interstitial tissue in the southern hairy-nosed wombat Lasiorhinus latifrons was investigated. Unlike in most other marsupials, the outer cortical region of the ovary contains abundant luteinised interstitial tissue that largely occurs in discrete lobules, many of which contain a localised area of non-cellular, highly eosinophilic and periodic acid-Schiff-positive material. The findings suggest that the latter arises from the zona pellucida that surrounded the oocyte in growing follicles and that the luteinised interstitial tissue thus developed from transformed theca interna of degenerated atretic follicles. It is hypothesised that this tissue synthesises and secretes progestogens, which may result in the long, and variable, oestrous cycle length that has been found to occur in this species.

The Haemocytes and Connective Tissue Formation in an Insect, Rhodnius Prolixus (Hemiptera)

1956 ◽  
Vol s3-97 (37) ◽  
pp. 89-98
Author(s):  
V. B. WIGGLESWORTH
Keyword(s):  
Connective Tissue ◽  
Basement Membrane ◽  
Blood Cell ◽  
Periodic Acid ◽  
Rhodnius Prolixus ◽  
Ground Substance ◽  
Muscle Fibres ◽  
Tissue Formation ◽  
Periodic Acid Schiff ◽  
Positive Material

The amoebocytes, which form the most abundant type of blood-cell in Rhodnius, contain rounded, oval, or rod-shaped inclusions which stain by the periodic acid/Schiff method. These are believed to be neutral mucopolysaccharides. The amoebocytes apply themselves to the basal membranes, which likewise are PAS-positive, and appear to contribute to these membranes by discharging their contents. They insinuate themselves into the developing muscles and give rise to the sheaths of connective tissue by which the muscle fibres are surrounded. And they collect around deposits of injected Indian ink, producing similar sheets of PAS-positive material, presumably mucopolysaccharide. The chitinous endocuticle is PAS-negative (except in a few special regions such as the neck and the conjunctival membranes of the limbs). It becomes positive during digestion by the moulting fluid. The tracheae react similarly. Other PAS-positive structures which are not produced by the amoebocytes are the striated border of the mid-gut, the basement membrane of the gut, the perilemma around the ganglia and nerves, and the ground substance within the ganglia.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Detection of Carriers in Glanzmann's Thrombasthenia

1983 ◽  
Vol 49 (03) ◽  
pp. 182-186
Author(s):  
G T E Zonneveld ◽  
E F van Leeuwen ◽  
A Sturk ◽  
J W ten Cate
Keyword(s):  
Bleeding Time ◽  
Periodic Acid ◽  
Type I ◽  
Clot Retraction ◽  
Periodic Acid Schiff ◽  
Glanzmann’S Thrombasthenia ◽  
Aggregation Response ◽  
Glanzmann's Thrombasthenia ◽  
Sds Page ◽  
Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity

2020 ◽  
Vol 138 ◽  
pp. 237-246 ◽  
Author(s):  
J Řehulka ◽  
A Kubátová ◽  
V Hubka
Keyword(s):  
Rainbow Trout ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Bladder Wall ◽  
Swim Bladder ◽  
Cell Reactivity ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Hepatic Congestion ◽  
Phoma Herbarum

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bladder contents revealed abundant septate hyphae, and histopathological examination showed periodic acid-Schiff-positive mycelia in the lumen and wall of the swim bladder. Histopathological examination of the thickened posterior swim bladder revealed muscle hyperplasia with expansion by inflammatory cells. The causative agent was identified as Phoma herbarum through morphological analysis and DNA sequencing. The disease was reproduced in rainbow trout fingerlings using intraperitoneal injection of a spore suspension. Necropsy in dead and moribund fish revealed extensive congestion and haemorrhages in the serosa of visceral organs and in liver and abdominal serosanguinous fluid. Histopathological examination showed severe hepatic congestion, sinusoidal dilatation, Kupffer cell reactivity, leukostasis and degenerative changes. Fungi were disseminated to the liver, pyloric caeca, kidney, spleen and heart. Although infections caused by Phoma spp. have been repeatedly reported in fish, species identification has been hampered by extensive taxonomic changes. The results of this study confirmed the pathogenicity of P. herbarum in salmonids by using a reliably identified strain during experimental fish infection and provides new knowledge regarding the course of infection.

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