Relation between Ribonuclease Activity and Ribonucleic Acid Content

Nature ◽  
1957 ◽  
Vol 180 (4594) ◽  
pp. 1048-1049 ◽  
Author(s):  
L. LEDOUX ◽  
A. PILERI ◽  
F. VANDERHAEGHE ◽  
S. BRÄNDLI
Keyword(s):  
Ribonucleic Acid ◽  
Acid Content ◽  
Ribonuclease Activity

Ribonuclease Activity and Ribonucleic Acid Content of Homologous Human Normal and Cancer Cells

Nature ◽  
1958 ◽  
Vol 181 (4613) ◽  
pp. 913-914 ◽  
Author(s):  
L. LEDOUX ◽  
S. BRÄNDLI ◽  
J. C. DE PAEPE
Keyword(s):  
Cancer Cells ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Ribonuclease Activity

RIBONUCLEASE ACTIVITY OF FISH MUSCLE EXTRACTS

1958 ◽  
Vol 36 (1) ◽  
pp. 633-643 ◽  
Author(s):  
Neil Tomlinson
Keyword(s):  
Ribonucleic Acid ◽  
Fish Muscle ◽  
Ribonuclease Activity ◽  
Phosphodiesterase Activity ◽  
Ph Optimum ◽  
Ophiodon Elongatus ◽  
Zone Electrophoresis

A ribonuclease was partially purified from muscle of lingcod (Ophiodon elongatus). The preparation degraded yeast and lingcod ribonucleic acid. Four 3′-mononucleotides were identified as digestion products. The enzyme was readily destroyed by heat and acid (10 minutes at 58 °C, pH 2.5), and was inhibited strongly by Zn++, Cu++, monoiodoacetate, formaldehyde, and NaF. Its pH optimum was 6.5. Ribonuclease activity was also demonstrated in muscle extracts from spring salmon, lemon sole, dogfish, sculpin, herring, and swordfish. The muscle extracts of all fish examined possessed phosphodiesterase activity. The ribonuclease in lingcod extract could be separated from two phosphodiesterases also present by zone electrophoresis.

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