Effect of 2,4-Dichlorophenoxyacetic Acid and Maleic Hydrazide on Potato Proteins as shown by Paper Electrophoresis

Nature ◽  
1955 ◽  
Vol 176 (4491) ◽  
pp. 1029-1030 ◽  
Author(s):  
GRACE K. YASUDA ◽  
MERLE G. PAYNE ◽  
JESS L. FULTS
Keyword(s):  
Maleic Hydrazide ◽  
Paper Electrophoresis ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

scholarly journals Changes in Leaf-Sugar Content and Enzyme Activity of Immature Sugarcane Following Foliar Application of Indole-3-Acetic Acid, 2,4-Dichlorophenoxyacetic Acid, and Maleic Hydrazide

1969 ◽  
Vol 49 (1) ◽  
pp. 1-34
Author(s):  
Alex G. Alexander
Keyword(s):  
Acetic Acid ◽  
Maleic Hydrazide ◽  
Foliar Application ◽  
Sugar Content ◽  
Water Soluble ◽  
Dichlorophenoxyacetic Acid ◽  
Enzyme Preparations ◽  
Freeze Dried ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid, and maleic hydrazide were applied as foliar sprays to 10-week-old sugarcane plants during initial studies of the interrelationships of growth-regulating materials with the sugar-metabolizing enzymes of sugarcane. Leaf samples were harvested at 1, 3, 9, and 27 days following treatment for sugar and enzyme assays. Sugar analyses were run for total ketoses, sucrose, fructose, and total reducing sugars, with glucose being determined by calculation. A series of acid phosphatase assays were conducted using as substrates the following compounds: ß-glycerophosphate, adenosinetriphosphate, uridine diphosphate glucose, glucose-1-phosphate, glucose-6-phosphate, fructose-6- phosphate, fructose-1,6-diphosphate, and 3-phosphoglyceric acid. Additional enzymes included invertase, amylase, hexokinase, phosphohexose isomerase, aldolase, triosephosphate dehydrogenase, phosphoglyceryl kinase, condensing enzyme, isocitric acid dehydrogenase, transaminase, peroxidase, and glucose oxidase. All enzyme preparations consisted of dialyzed water-soluble protein extracted from freeze-dried leaf tissue and precipitated with ammonium sulfate between 35 and 95 percent of saturation.

THE EFFECT OF MALEIC HYDRAZIDE ON SYNTHESIS OF LIGNIN

1964 ◽  
Vol 44 (3) ◽  
pp. 253-258 ◽  
Author(s):  
E. V. Parups
Keyword(s):  
Gibberellic Acid ◽  
Ferulic Acid ◽  
Ultraviolet Light ◽  
Light Absorption ◽  
Maleic Hydrazide ◽  
Lignin Content ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

The effect of maleic hydrazide and several other chemicals on the synthesis of lignin was investigated in oat coleoptile, pea hypocotyl, and tobacco. When measured by ultraviolet light absorption, maleic hydrazide depressed While 1,4-naphthoquinone increased the lignin content in the conducting tissues of oat coleoptile. The lignin content of oat coleoptiles treated with maleic hydrazide in presence of 1,4-naphthoquinone and tyrosine was reduced. Additions of ferulic acid generally overcame the effects of maleic hydrazide; p-benzoquinone was ineffective. Gibberellic acid and 2,4-dichlorophenoxyacetic acid generally promoted the synthesis of lignin. Joint use of these chemicals and maleic hydrazide produced intermediate levels of lignin. The hygroscopicity and filling value of flue-cured tobacco was related to the lignin content of the leaf.

Enhancement by Osmotic Treatment of Hairy Root Transformation of Alfalfa Suspension Cultures, and Chromosomal Variation in the Transformed Tissues

1997 ◽  
Vol 24 (3) ◽  
pp. 345 ◽  
Author(s):  
Zi-Qin Xu ◽  
Jing-Fen Jia ◽  
Zhi-De Hu
Keyword(s):  
Chromosomal Abnormalities ◽  
Paper Electrophoresis ◽  
Suspension Cultures ◽  
Transformation Frequency ◽  
Dichlorophenoxyacetic Acid ◽  
Structural Alterations ◽  
Root Transformation ◽  
Selection Medium ◽  
Osmotic Treatment ◽  
2,4 Dichlorophenoxyacetic Acid

Seeds of alfalfa were germinated on Murashige and Skoog medium without phytohormones. The hypocotyls ofseedlings were excised and cultured on the same medium with 2,4- dichlorophenoxyacetic acid (9.05 mM) to induce callus. Granular calluses were suspended and cultured in Schenk and Hildebrandt medium supplemented with 2.26 mM 2,4-dichlorophenoxyacetic acid on a shaker at 80 rpm. Alfalfa suspension cultures were treated with 0.45 M mannitol for 1 h, and washed twice with 0.16 M CaCl2 .2H2 O. After pretreatment, the pellets were resuspended in Schenk and Hildebrandt medium without phytohormone (10 mL g-1 suspension cultures), and 0.2 mL of Agrobacterium rhizogenes suspension was added. A mixture of alfalfa cells and Agrobacterium was co-cultured at 25 ± 2˚C for 2 days. Transformants (transformed calluses and hairy roots) were obtained by hormone-free selection. Several factors, such as culturing stage of suspension cultures, phytohormone constitution of suspension medium and basal selection medium of transformants, affected the transformation frequency remarkably. Paper electrophoresis revealed that over 70% of transformants could synthesise agropine and mannopine. A comparative cytological analysis revealed the number and structural alterations of chromosomes in the resulting materials. The transformed tissue was not able to be regenerated, possibly due to chromosomal abnormalities.

Effect of Maleic Hydrazide and Other Plant Growth Regulators on the Pea Aphid, Acyrthosiphon pisum (Harris), Caged on Broad Bean, Vicia faba L

1960 ◽  
Vol 92 (7) ◽  
pp. 494-499 ◽  
Author(s):  
A. G. Robinson
Keyword(s):  
Plant Growth ◽  
Vicia Faba ◽  
Acyrthosiphon Pisum ◽  
Maleic Hydrazide ◽  
Broad Bean ◽  
Pea Aphid ◽  
Dichlorophenoxyacetic Acid ◽  
Root Absorption ◽  
Chlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

In a preliminary report (Robinson, 1959) it was shown that the plant growth regulator maleic hydrazide (MH) caused both nymphal mortality and reduced fecundity to the pea aphid, Acyrthosiphon pisum (Harris), caged on treated broad bean, Vicia faba L. It was also stated that 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), and 2-methyl-4-chlorophenoxyacetic acid (MCPA), applied by root absorption to broad bean had no effect on adult or nymphal mortality, or fecundity of adult A. pisum, at the rates used.

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

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