Use of n-Butanol - Formic Acid - Water Mixture in the Paper Chromatography of Amino-Acids and Sugars

Nature ◽  
1952 ◽  
Vol 170 (4320) ◽  
pp. 279-280 ◽  
Author(s):  
L. F. WIGGINS ◽  
J. HOWARTH WILLIAMS
1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.


2006 ◽  
Vol 128 (6) ◽  
pp. 2030-2034 ◽  
Author(s):  
Volker Knecht ◽  
Alan E. Mark ◽  
Siewert-Jan Marrink

1954 ◽  
Vol 58 (5) ◽  
pp. 595-601 ◽  
Author(s):  
Burnham S. Walker ◽  
Norman Telles ◽  
Edward Pastore

1956 ◽  
Vol 1 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Jorge Awapara ◽  
Yukiko Sato

1968 ◽  
Vol 37 ◽  
pp. 545-546 ◽  
Author(s):  
Brother Thomas McCullough ◽  
Edward DeJong ◽  
Brother Chester Caster

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