Intake of NaCl solution in rats with diabetes insipidus.

1969 ◽  
Vol 68 (1, Pt.1) ◽  
pp. 38-44 ◽  
Author(s):  
Genaro M. Palmieri ◽  
Samuel Taleisnik
Keyword(s):  
Diabetes Insipidus ◽  
Nacl Solution

Altered acceptance and rejection of NaCl in rats with diabetes insipidus

1960 ◽  
Vol 199 (1) ◽  
pp. 22-24 ◽  
Author(s):  
Libby F. Titlebaum ◽  
John L. Falk ◽  
Jean Mayer
Keyword(s):  
Diabetes Insipidus ◽  
Serum Sodium ◽  
Sodium Level ◽  
Serum Sodium Level ◽  
Extracellular Fluid ◽  
Solution Concentration ◽  
Lower Solution ◽  
Extracellular Fluid Volume ◽  
Internal Environment ◽  
Nacl Solution

Groups of normal rats and animals with diabetes insipidus were tested on their relative acceptance and rejection of various concentrations of NaCl solution. Rats with diabetes inspidus drank more than normal rats in the hypotonic range. Their acceptance was maximal at a lower solution concentration than in the case of normal animals and relative rejection began at concentrations where normals ingested maximally. The results are discussed in relation to such factors in the internal environment as serum sodium level and extracellular fluid volume.

Effect of Age on Hypertensive Stimuli and the Development of Hypertension in Brattleboro Rats

1979 ◽  
Vol 57 (3) ◽  
pp. 273-275 ◽  
Author(s):  
H. Dlouhá ◽  
J. Křeček ◽  
J. Zicha
Keyword(s):  
Blood Pressure ◽  
Diabetes Insipidus ◽  
Unilateral Nephrectomy ◽  
Brattleboro Rats ◽  
Nacl Solution ◽  
Adult Rats ◽  
Saline Administration ◽  
Solution Saline ◽  
Effect Of Age ◽  
Inherited Diabetes

1. In rats with inherited diabetes insipidus, unilateral nephrectomy plus drinking of 0·6% NaCl solution (saline) did not influence blood pressure in adult rats. However, when these factors applied before puberty, they produced hypertension. 2. We therefore analysed whether saline administration before puberty or unilateral nephrectomy before puberty was more important for this hypertensive response. 3. Saline drinking was found to be necessary for the response because hypertension was elicited by unilateral nephrectomy in adult rats only if saline consumption began before puberty.

Effect of Sodium Loading on the Urinary Excretion of Prostaglandins E2 and F2α in Rats with Hereditary Diabetes Insipidus (Brattleboro Rats)

1985 ◽  
Vol 68 (1) ◽  
pp. 93-96 ◽  
Author(s):  
M. Rathaus ◽  
N. Kariv ◽  
J. Shapira ◽  
J. Bernheim
Keyword(s):  
Urinary Excretion ◽  
Diabetes Insipidus ◽  
Sodium Intake ◽  
Current Evidence ◽  
Sodium Balance ◽  
Brattleboro Rats ◽  
Nacl Solution ◽  
Salt Loading ◽  
Sodium Loading ◽  
Hereditary Diabetes

1. Current evidence suggests that the antidiuretic hormone (ADH) and changes in sodium balance influence renal prostaglandins (PGs). To separate these two mechanisms, the effect of sodium loading on the urinary excretion of PGE2 and PGF2α was studied in female Brattleboro rats with diabetes insipidus (DIHO) and compared with that in female, age matched, heterozygous Long Evans controls (LEHE). 2. Ten DIHO and ten LEHE rats had a normal sodium intake. In ten DIHO rats a 0.16% NaCl solution was supplied instead of drinking water for either 8 days (n = 5) or 14 days (n = 5). In two groups of LEHE rats, sodium loading was obtained with a 0.80% NaCl solution for the same study periods. Urine PGs were measured by radioimmunoassay in three 24 h urine collections for each rat. 3. Urine PGs were significantly increased in the 8 day loaded but not in the 14 day loaded LEHE rats. In DIHO rats, a non-significant increase in both PGE2 and PGF2α was present after 8 days of sodium loading, while PGE2 and the E/F ratio were decreased after 14 days of salt loading. 4. The findings suggest that the natriuresis induced by sodium loading in the rat may be mediated in part by increased production of PGs. In addition, it seems that ADH plays a role in this response.

Effect of ADH on Rat Renal Papilla, an Ultrastructural and Cytochemical Study

1973 ◽  
Vol 31 ◽  
pp. 410-411
Author(s):  
C. N. Sun ◽  
H. J. White ◽  
E. J. Towbin
Keyword(s):  
Electron Microscopy ◽  
Diabetes Insipidus ◽  
Renal Papilla ◽  
Milk Diet ◽  
Intercellular Spaces ◽  
Cytochemical Study ◽  
Collecting Tubule ◽  
Concentrate Urine ◽  
Staining Techniques ◽  
Collecting Tubules

Diabetes insipidus and compulsive water drinking are representative of two categories of antidiuretic hormone (ADH) lack. We studied a strain of rats with congenital diabetes insipidus homozygote (DI) and normal rats on an isocaloric fortified dilute milk diet. In both cases, the collecting tubules could not concentrate urine. Special staining techniques, Alcian Blue-PAS for light microscopy and lanthanum nitrate for electron microscopy were used to demonstrate the changes in interstitial mucopolysaccharides (MPS). The lanthanum staining was done according to the method of Khan and Overton.Electron microscopy shows cytoplasmic lesions, vacules, swelling and degenerating mitochondria and intercellular spaces (IS) in the collecting tubule cells in DI and rats on milk diet.

Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

1978 ◽  
Vol 36 (2) ◽  
pp. 310-311
Author(s):  
W. Liebrich
Keyword(s):  
Hela Cells ◽  
Calf Serum ◽  
Glass Tube ◽  
Serum Free Medium ◽  
Nacl Solution ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Serum Free ◽  
All Solutions ◽  
Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

Reactivity of Pulmonary Alveolar Cells to Crocidolite Asbestos Fibers

1982 ◽  
Vol 40 ◽  
pp. 334-335
Author(s):  
Charles L. Sanders ◽  
Roy R. Adee
Keyword(s):  
Osmium Tetroxide ◽  
Intratracheal Instillation ◽  
Uranyl Acetate ◽  
Fiber Suspension ◽  
Nacl Solution ◽  
Alveolar Cells ◽  
Asbestos Fibers ◽  
Fischer Rats ◽  
Mineral Silicates

Asbestos is a generic name for a group of hydrated mineral silicates that occur naturally in a fibrous form. The early interactions of asbestos fibers with alveolar cells in large part determines their long-term toxicity. Young adult, SPF, Fischer rats were given a single intratracheal instillation of 2 mg crocidolite asbestos suspended in 0.5 ml of 0.9% NaCl solution. About 80% of the fibers had lengths of less than 10 ym as measured on light micrographs of the fiber suspension. Two rats were killed at 3 hr, 1 d and 1, 4, 8, 12 and 16 wk after instillation and the lungs instilled with 8 ml McDowell - Trumps at 20 cm H2O. Lung tissue was dehydrated and sputtered coated with palladium-gold for SEM or post-fixed in osmium tetroxide, embedded in epoxy resin and sections stained with uranyl acetate and lead citrate for TEM.

Abstract #813 Adult Atypical Sellar Teratoid Tumor Presenting as Diabetes Insipidus

2018 ◽  
Vol 24 ◽  
pp. 178-179
Author(s):  
Sabrina Huq ◽  
Mahalakshmi Honasoge ◽  
Ebru Sulanc
Keyword(s):  
Diabetes Insipidus
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