Growth and nocturnal acid accumulation during early ontogeny of Agave attenuata grown in nutrient solution and in vitro culture

1997 ◽  
Vol 39 (1) ◽  
pp. 1-11 ◽  
Author(s):  
H. Wen ◽  
J. Wagner ◽  
W. Larcher
Keyword(s):  
In Vitro Culture ◽  
Nutrient Solution ◽  
Early Ontogeny ◽  
Acid Accumulation ◽  
Nocturnal Acid Accumulation

Initiation of roots on hypocotyl cuttings of Pinussylvestris, with emphasis on direct rooting, root elongation, and auxin uptake

1988 ◽  
Vol 18 (11) ◽  
pp. 1457-1462 ◽  
Author(s):  
Roland Grönroos ◽  
Sara von Arnold
Keyword(s):  
In Vitro Culture ◽  
Nutrient Solution ◽  
Acid Treatment ◽  
Root Elongation ◽  
Hydroponic Culture ◽  
Indolylacetic Acid ◽  
Auxin Uptake ◽  
Hypocotyl Cuttings ◽  
Wound Tissue

Direct rooting of hypocotyl cuttings of Pinussylvestris L. is described and compared with rooting via wound tissue. The optimal 3-indolylbutyric acid treatment for direct rooting of Pinussylvestris hypocotyl cuttings under in vitro conditions was 1.23 mM for 24 h. This treatment resulted in 45% rooting within 3 weeks after cutting. During this period, only direct rooting was observed. Three to 4 weeks after cutting, roots developed either directly on the hypocotyl or via wound tissue. After the first month, rooting took place predominantly via wound tissue. The optimal 3-indolylbutyric acid treatment found for in vitro culture could successfully be used for hydroponic culture (under nonsterile conditions). In vitro culture inhibited root elongation. However, after transfer of rooted cuttings from in vitro to hydroponic culture, root elongation increased markedly. Uptake studies with 3-[5(n)-3H]indolylacetic acid mixed with the nutrient solution showed that auxin uptake by the cutting probably occurred mainly through uptake of the nutrient solution. After 24 h treatment with 1.43 mM 3-indolylacetic acid, the concentration taken up by the cuttings was 0.91 nmol/mg fresh weight. Differences between individual cuttings in auxin uptake could not explain the poor direct rooting of Pinussylvestris hypocotyl cuttings cultured in vitro.

scholarly journals Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Miguel Contreras Ramírez ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Contreras ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

scholarly journals Influence of concentration and ratio of macro elements in culture medium on the regeneration of grape meristems in vitro

2020 ◽  
Vol 25 ◽  
pp. 05005
Author(s):  
Anton Rebrov
Keyword(s):  
In Vitro Culture ◽  
Chronic Diseases ◽  
Nutrient Solution ◽  
Culture Medium ◽  
Total Content ◽  
Regenerative Capacity ◽  
Macro Elements ◽  
Grape Varieties

The results of studies on the development of culture medium for initiation grape meristems into in vitro culture in order to increase their regenerative capacity during recovery from chronic diseases are presented. In the improved medium, in comparison with analogs, the total content of macro salts was reduced and their ratio was optimized, taking into account their consumption and the characteristics of their absorption from the nutrient solution by the grape plant. In the course of testing the new culture medium, its effectiveness has been established for grape varieties from various ecological and geographical groups. A higher and more stable percentage of survival and regeneration of extremely small meristems have been established against the background of various modifications of the recipe for the traditionally used culture medium of Murashige and Skoog.

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens

Effect of antioxidants on in vitro culture of pomegranate cv. Sindhuri

2018 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
Ravi Kumar ◽  
◽  
M.L. Jakhar ◽  
Komal Sekhawat ◽  
Swarnlata Kumawat ◽  
...  
Keyword(s):  
In Vitro Culture

Leaf Color of Plants Regenerated through In Vitro Culture from Variegated Leaf Segments of Caladium.

1993 ◽  
Vol 62 (3) ◽  
pp. 619-624 ◽  
Author(s):  
Yu Zhu ◽  
Tetsuyuki Takemoto ◽  
Susumu Yazawa
Keyword(s):  
In Vitro Culture ◽  
Leaf Color ◽  
Leaf Segments ◽  
Variegated Leaf

In vitro Culture of Human PBMCs

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Santosh Panda ◽  
Balachandran Ravindran
Keyword(s):  
In Vitro Culture ◽  
Human Pbmcs
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