DNA Damage by Dimethylformamide:  Role of Hydrogen Peroxide Generated during Degradation

2000 ◽  
Vol 13 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Kaoru Midorikawa ◽  
Mariko Murata ◽  
Shinji Oikawa ◽  
Saeko Tada-Oikawa ◽  
Shosuke Kawanishi
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage

scholarly journals Role of Dipicolinic Acid in Resistance and Stability of Spores of Bacillus subtilis with or without DNA-Protective α/β-Type Small Acid-Soluble Proteins

2006 ◽  
Vol 188 (11) ◽  
pp. 3740-3747 ◽  
Author(s):  
Barbara Setlow ◽  
Swaroopa Atluri ◽  
Ryan Kitchel ◽  
Kasia Koziol-Dube ◽  
Peter Setlow
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Bacillus Subtilis ◽  
Dipicolinic Acid ◽  
Dry Weight ◽  
Definitive Evidence ◽  
Dry Heat ◽  
Spore Resistance ◽  
Wet Heat

ABSTRACT Dipicolinic acid (DPA) comprises ∼10% of the dry weight of spores of Bacillus species. Although DPA has long been implicated in spore resistance to wet heat and spore stability, definitive evidence on the role of this abundant molecule in spore properties has generally been lacking. Bacillus subtilis strain FB122 (sleB spoVF) produced very stable spores that lacked DPA, and sporulation of this strain with DPA yielded spores with nearly normal DPA levels. DPA-replete and DPA-less FB122 spores had similar levels of the DNA protective α/β-type small acid-soluble spore proteins (SASP), but the DPA-less spores lacked SASP-γ. The DPA-less FB122 spores exhibited similar UV resistance to the DPA-replete spores but had lower resistance to wet heat, dry heat, hydrogen peroxide, and desiccation. Neither wet heat nor hydrogen peroxide killed the DPA-less spores by DNA damage, but desiccation did. The inability to synthesize both DPA and most α/β-type SASP in strain PS3664 (sspA sspB sleB spoVF) resulted in spores that lost viability during sporulation, at least in part due to DNA damage. DPA-less PS3664 spores were more sensitive to wet heat than either DPA-less FB122 spores or DPA-replete PS3664 spores, and the latter also retained viability during sporulation. These and previous results indicate that, in addition to α/β-type SASP, DPA also is extremely important in spore resistance and stability and, further, that DPA has some specific role(s) in protecting spore DNA from damage. Specific roles for DPA in protecting spore DNA against damage may well have been a major driving force for the spore's accumulation of the high levels of this small molecule.

On the role of iron and copper ions in hydrogen peroxide-induced cellular DNA damage

2002 ◽  
Vol 32 (2) ◽  
pp. 198-199 ◽  
Author(s):  
Dimitrios Galaris ◽  
Ben-Zhan Zhu ◽  
Balz Frei
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Copper Ions ◽  
Cellular Dna

scholarly journals Role of Trypanosoma cruzi nucleoside diphosphate kinase 1 in DNA-damage responses

2019 ◽  
Author(s):  
Chantal Reigada ◽  
Melisa Sayé ◽  
Fabio Di Girolamo ◽  
Edward A. Valera-Vera ◽  
Claudio A. Pereira ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Dna Repair ◽  
Trypanosoma Cruzi ◽  
Mammalian Cells ◽  
Spontaneous Mutation ◽  
Nucleoside Diphosphate Kinase ◽  
Dna Integrity ◽  
Dna Damage Responses

AbstractNME23/NDPK proteins are well conserved proteins found in all living organism. Besides their catalytic activity of nucleoside diphosphate kinase (NDPK) they are considered multifunctional, which were first characterized as non-metastatic proteins in mammalian cells. Later, increasing evidences placed NME/NDPK as proteins involved in DNA stability such as gene regulation and DNA-repair. TcNDPK1 is the canonical NDPK isoform present in the parasite Trypanosoma cruzi, orthologous to NME23-H1/H2 which has been shown to have in vitro nuclease activity and DNA-binding properties. In the present study we investigate the role of TcNDPK1 in DNA-damage responses using heterologous gene expression systems and over-expression in epimastigote cells. We found that different strains of bacteria, WT and ndk-mutants, expressing the enzyme decreased about 5 fold and 18 fold the spontaneous mutation rate, respectively. In addition, yeasts lacking the endogenous gene YNK1 (YNK1-) and expressing TcNDPK1, were significantly more resistant to different concentrations of hydrogen peroxide and were less sensible to UV radiation than controls. Parasites over-expressing TcNDPK1 were able to withstand different genotoxic stresses caused by hydrogen peroxide, phleomycin and hidroxyurea. In addition, under oxidative damage, TcNDPK1 over-expressing parasites presented lesser genomic damage and augmented levels of poly(ADP)ribose and poly(ADP)ribose polymerase, an enzyme involved in DNA repair. These results strongly suggest that TcNDPK1 is involved in the maintenance of parasite genomic-DNA integrity, thus, giving rise to a novel function.

scholarly journals Oxidative DNA Damage and Apoptosis Induced by Aclarubicin, an Anthracycline: Role of Hydrogen Peroxide and Copper

2019 ◽  
Vol 39 (7) ◽  
pp. 3443-3451 ◽  
Author(s):  
HIDEKI MIZUTANI ◽  
YUKA HAYASHI ◽  
MIYABI HASHIMOTO ◽  
MASANORI IMAI ◽  
YOSHIMI ICHIMARU ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Oxidative Dna Damage
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