scholarly journals Formation and Repair of Pyridyloxobutyl DNA Adducts and Their Relationship to Tumor Yield in A/J Mice

2012 ◽  
Vol 25 (10) ◽  
pp. 2167-2178 ◽  
Author(s):  
Anna M. Urban ◽  
Pramod Upadhyaya ◽  
Qing Cao ◽  
Lisa A. Peterson
Keyword(s):  
Dna Adducts ◽  
Tumor Yield

Cytochrome P4502E1 is responsible for miscoding etheno-Dna adducts induced by ethanol in the liver

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
Y Wang ◽  
J Nair ◽  
S Mueller ◽  
F Stickel ◽  
H Bartsch ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Cytochrome P4502e1

scholarly journals Identification of New Markers of Alcohol-Derived DNA Damage in Humans

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 366
Author(s):  
Valeria Guidolin ◽  
Erik S. Carlson ◽  
Andrea Carrà ◽  
Peter W. Villalta ◽  
Laura A. Maertens ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Adducts ◽  
Neutral Loss ◽  
Alcohol Exposure ◽  
Dose Dependence ◽  
Accurate Mass ◽  
Constant Neutral Loss ◽  
Covalent Modifications ◽  
Underlying Mechanisms

Alcohol consumption is a risk factor for the development of several cancers, including those of the head and neck and the esophagus. The underlying mechanisms of alcohol-induced carcinogenesis remain unclear; however, at these sites, alcohol-derived acetaldehyde seems to play a major role. By reacting with DNA, acetaldehyde generates covalent modifications (adducts) that can lead to mutations. Previous studies have shown a dose dependence between levels of a major acetaldehyde-derived DNA adduct and alcohol exposure in oral-cell DNA. The goal of this study was to optimize a mass spectrometry (MS)-based DNA adductomic approach to screen for all acetaldehyde-derived DNA adducts to more comprehensively characterize the genotoxic effects of acetaldehyde in humans. A high-resolution/-accurate-mass data-dependent constant-neutral-loss-MS3 methodology was developed to profile acetaldehyde-DNA adducts in purified DNA. This resulted in the identification of 22 DNA adducts. In addition to the expected N2-ethyldeoxyguanosine (after NaBH3CN reduction), two previously unreported adducts showed prominent signals in the mass spectra. MSn fragmentation spectra and accurate mass were used to hypothesize the structure of the two new adducts, which were then identified as N6-ethyldeoxyadenosine and N4-ethyldeoxycytidine by comparison with synthesized standards. These adducts were quantified in DNA isolated from oral cells collected from volunteers exposed to alcohol, revealing a significant increase after the exposure. In addition, 17 of the adducts identified in vitro were detected in these samples confirming our ability to more comprehensively characterize the DNA damage deriving from alcohol exposures.

scholarly journals Mutagenesis by single site-specific arylamine-DNA adducts

1989 ◽  
Vol 264 (33) ◽  
pp. 20120-20130
Author(s):  
P K Gupta ◽  
D L Johnson ◽  
T M Reid ◽  
M S Lee ◽  
L J Romano ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Single Site ◽  
Site Specific

scholarly journals Repair of aflatoxin B1 DNA adducts by the UvrABC endonuclease of Escherichia coli.

1993 ◽  
Vol 268 (11) ◽  
pp. 7990-8002
Author(s):  
C.A. Oleykowski ◽  
J.A. Mayernik ◽  
S.E. Lim ◽  
J.D. Groopman ◽  
L. Grossman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Adducts ◽  
Aflatoxin B1
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