Metabolic pathways of 1-butyl [3-13C]acrylate. Identification of urinary metabolites in rat using nuclear magnetic resonance and mass spectroscopy

1994 ◽  
Vol 7 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Igor Linhart ◽  
Richard Hrabal ◽  
Jaroslav Smejkal ◽  
Jiri Mitera
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Mass Spectroscopy ◽  
Metabolic Pathways ◽  
Urinary Metabolites ◽  
Nuclear Magnetic

Elucidation of metabolic pathways in glycogen-accumulating organisms with in vivo13C nuclear magnetic resonance

2007 ◽  
Vol 9 (11) ◽  
pp. 2694-2706 ◽  
Author(s):  
Paulo C. Lemos ◽  
Yu Dai ◽  
Zhiguo Yuan ◽  
Jurg Keller ◽  
Helena Santos ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Metabolic Pathways ◽  
Nuclear Magnetic

scholarly journals A systematic review of the small molecule studies of osteoarthritis using nuclear magnetic resonance and mass spectroscopy

2019 ◽  
Vol 27 (4) ◽  
pp. 560-570 ◽  
Author(s):  
M.K.J. Jaggard ◽  
C.L. Boulangé ◽  
P. Akhbari ◽  
U. Vaghela ◽  
R. Bhattacharya ◽  
...  
Keyword(s):  
Systematic Review ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Small Molecule ◽  
Mass Spectroscopy ◽  
Nuclear Magnetic

Immunosuppressive activity of cyclosporine metabolites compared and characterized by mass spectroscopy and nuclear magnetic resonance

1990 ◽  
Vol 36 (2) ◽  
pp. 225-229 ◽  
Author(s):  
K R Copeland ◽  
R W Yatscoff ◽  
R M McKenna
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Mass Spectroscopy ◽  
Proton Nuclear Magnetic Resonance ◽  
Therapeutic Monitoring ◽  
Potency Ratio ◽  
Transplant Patients ◽  
In Vitro Systems ◽  
Nuclear Magnetic

Abstract Eight cyclosporine (CsA) metabolites were isolated from the urine of renal-transplant patients by high-pressure liquid chromatography. Structure and purity of the metabolites were assessed by fast atomic bombardment/mass spectroscopy, by proton nuclear magnetic resonance (NMR), and, when the quantity of metabolites permitted, by 13C-NMR. The immunosuppressive activities (I) of the metabolites were tested in three separate in vitro systems: primary and secondary mixed lymphocyte reactions as well as by a mitogen-stimulated system. The I, as measured by comparing the concentration of each metabolite required for 50% inhibition of incorporation of [3H] thymidine, varied among the assay systems, as did the ranking of I among the test systems. In general, the I of most metabolites in all assay systems were less than 10% of that for CsA. Metabolites with single modifications exhibited the greatest I; e.g., that of M-17 was congruent to 16% of that of CsA (potency ratio 0.16) in a secondary mixed lymphocyte reaction. The significance of these findings in relation to therapeutic monitoring of CsA is discussed.

scholarly journals Catabolism of Glucose and Lactose in Bifidobacterium animalis subsp. lactis, Studied by13C Nuclear Magnetic Resonance

2013 ◽  
Vol 79 (24) ◽  
pp. 7628-7638 ◽  
Author(s):  
Irene González-Rodríguez ◽  
Paula Gaspar ◽  
Borja Sánchez ◽  
Miguel Gueimonde ◽  
Abelardo Margolles ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Metabolic Pathways ◽  
Transcriptional Analysis ◽  
Content Type ◽  
Bifidobacterium Animalis ◽  
Metabolic Flux Distribution ◽  
Nuclear Magnetic ◽  
In Cells

ABSTRACTBifidobacteria are widely used as probiotics in several commercial products; however, to date there is little knowledge about their carbohydrate metabolic pathways. In this work, we studied the metabolism of glucose and lactose in the widely used probiotic strainBifidobacterium animalissubsp.lactisBB-12 byin vivo13C nuclear magnetic resonance (NMR) spectroscopy. The metabolism of [1-13C]glucose was characterized in cells grown in glucose as the sole carbon source. Moreover, the metabolism of lactose specifically labeled with13C on carbon 1 of the glucose or the galactose moiety was determined in suspensions of cells grown in lactose. These experiments allowed the quantification of some intermediate and end products of the metabolic pathways, as well as determination of the consumption rate of carbon sources. Additionally, the labeling patterns in metabolites derived from the metabolism of glucose specifically labeled with13C on carbon 1, 2, or 3 in cells grown in glucose or lactose specifically labeled in carbon 1 of the glucose moiety ([1-13Cglucose]lactose), lactose specifically labeled in carbon 1 of the galactose moiety ([1-13Cgalactose]lactose), and [1-13C]glucose in lactose-grown cells were determined in cell extracts by13C NMR. The NMR analysis showed that the recovery of carbon was fully compatible with the fructose 6-phosphate, or bifid, shunt. The activity of lactate dehydrogenase, acetate kinase, fructose 6-phosphate phosphoketolase, and pyruvate formate lyase differed significantly between glucose and lactose cultures. The transcriptional analysis of several putative glucose and lactose transporters showed a significant induction of Balat_0475 in the presence of lactose, suggesting a role for this protein as a lactose permease. This report provides the firstin vivoexperimental evidence of the metabolic flux distribution in the catabolic pathway of glucose and lactose in bifidobacteria and shows that the bifid shunt is the only pathway involved in energy recruitment from these two sugars. On the basis of our experimental results, a model of sugar metabolism inB. animalissubsp.lactisis proposed.

scholarly journals Combining reverse genetics and nuclear magnetic resonance-based metabolomics unravels trypanosome-specific metabolic pathways

2015 ◽  
Vol 96 (5) ◽  
pp. 917-926 ◽  
Author(s):  
Frédéric Bringaud ◽  
Marc Biran ◽  
Yoann Millerioux ◽  
Marion Wargnies ◽  
Stefan Allmann ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Metabolic Pathways ◽  
Reverse Genetics ◽  
Nuclear Magnetic

Metabolomics and protozoan parasites

2013 ◽  
Vol 58 (2) ◽  
Author(s):  
Timothy Paget ◽  
Nicolas Haroune ◽  
Sushmita Bagchi ◽  
Edward Jarroll
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Mass Spectroscopy ◽  
State Of The Art ◽  
The State ◽  
Protozoan Parasites ◽  
Gas And Liquid Chromatography ◽  
Nuclear Magnetic

AbstractIn this review, we examine the state-of-the-art technologies (gas and liquid chromatography, mass spectroscopy and nuclear magnetic resonance, etc.) in the well-established area of metabolomics especially as they relate to protozoan parasites.

Polyprenols of Listeria monocytogenes

1973 ◽  
Vol 51 (6) ◽  
pp. 939-941 ◽  
Author(s):  
A. Vilim ◽  
M. C. Woods ◽  
K. K. Carroll
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Listeria Monocytogenes ◽  
Magnetic Resonance ◽  
Mass Spectroscopy ◽  
Nuclear Magnetic

Listeria monocytogenes was found to contain polyprenols in a concentration of 215 mg/kg of lyophilized bacteria. They consisted mainly of undecaprenol with a small amount of decaprenol. Characterization by infrared, nuclear magnetic resonance, and mass spectroscopy indicated that the undecaprenol had the same structure as that isolated from other bacteria.

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