Characterization of 2‘-Deoxyadenosine Adducts Derived from 4-Oxo-2-nonenal, a Novel Product of Lipid Peroxidation

2000 ◽  
Vol 13 (7) ◽  
pp. 565-574 ◽  
Author(s):  
Seon Hwa Lee ◽  
Diane Rindgen ◽  
Roy H. Bible, ◽  
Elisabeth Hajdu ◽  
Ian A. Blair
Keyword(s):  
Lipid Peroxidation ◽  
Deoxyadenosine Adducts

scholarly journals Isolation and characterization of a rat cDNA clone encoding a secreted superoxide dismutase reveals the epididymis to be a major site of its expression

1993 ◽  
Vol 293 (1) ◽  
pp. 21-25 ◽  
Author(s):  
A C F Perry ◽  
R Jones ◽  
L Hall
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Sequence Analysis ◽  
Cdna Clone ◽  
Northern Blot ◽  
Northern Blot Analysis ◽  
Major Site ◽  
Isolation And Characterization

Superoxide dismutase (SOD) plays a key role in combating loss of fertility of spermatozoa due to lipid peroxidation. Here we report the sequence of a cDNA encoding a secreted form of SOD isolated from a rat epididymal library. Northern-blot analysis indicates that the corresponding transcript is expressed principally in the cauda region of the epididymis, consistent with the high levels of SOD enzyme activity found in cauda-epididymidal plasma. Much lower levels of an identically sized transcript exist in all tissues examined, including placenta. PCR and subsequent sequence analysis of rat placental SOD strongly suggest that it is identical in sequence with epididymal SOD.

scholarly journals Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1589
Author(s):  
Dragana D. Antonić ◽  
Angelina R. Subotić ◽  
Milan B. Dragićević ◽  
Danijel Pantelić ◽  
Snežana M. Milošević ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Salicylic Acid ◽  
Drought Stress ◽  
De Novo ◽  
Foliar Application ◽  
Drought Response ◽  
Leaf Transcriptome ◽  
Relative Water ◽  
Impatiens Walleriana

Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.

CBIO-03. A COMPREHENSIVE CHARACTERIZATION OF THE GBM LIPIDOME REVEALS A MOLECULARLY-DEFINED SUB-GROUP WITH HEIGHTENED SENSITIVITY TO FERROPTOSIS

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi27-vi27
Author(s):  
Danielle Morrow ◽  
Jenna Minami ◽  
Nicholas Bayley ◽  
Kevin Williams ◽  
Robert Prins ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Metabolism ◽  
Model Systems ◽  
Molecular Signatures ◽  
Molecular Alterations ◽  
Lipidomic Analysis ◽  
Novel Association ◽  
Therapeutic Opportunity ◽  
Comprehensive Characterization

Abstract Cancers, including the universally lethal glioblastoma (GBM), have reprogrammed lipid metabolism to fuel tumor growth. However, the molecular alterations responsible for aberrant lipid metabolism, and the potential for identifying new therapeutic opportunities are not fully understood. To systematically investigate the GBM lipidome, we performed integrated transcriptomic, genomic and shotgun lipidomic analysis of an extensive library of molecularly diverse patient-derived GBM tumors and model systems. Using this comprehensive approach, we discovered two GBM sub-groups defined by their combined molecular and lipidomic profile. Among the most significant differences between the two groups were lipid length and desaturation. As a consequence of this signature, a subset was more sensitive to lipid peroxidation and ferroptosis. Our findings suggest a novel association between specific molecular signatures of GBM, lipid metabolism and lipid peroxidation-induced cell death. This relationship may present a new therapeutic opportunity to target reprogrammed lipid metabolism in a molecularly-defined subset of GBMs.

scholarly journals Characterization of Blood Oxidative Stress in Type 2 Diabetes Mellitus Patients: Increase in Lipid Peroxidation and SOD Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Suziy de M. Bandeira ◽  
Glaucevane da S. Guedes ◽  
Lucas José S. da Fonseca ◽  
André S. Pires ◽  
Daniel P. Gelain ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Lipid Peroxidation ◽  
Diabetic Patients ◽  
Sod Activity ◽  
Glucose Levels ◽  
Significant Difference ◽  
Close Relationship

This study evaluated the oxidative stress through enzymatic and nonenzymatic biomarkers in diabetic patients with and without hypertension and prediabetics. The SOD and CAT (in erythrocytes) and GPx (in plasma) enzymatic activities, plasma levels of lipid peroxidation, and total thiols were measured in the blood of 55 subjects with type 2 diabetes and 38 subjects without diabetes (9 pre-diabetics and 29 controls) aged 40–86 years. The total SOD activity and the lipid peroxidation were higher in diabetics compared to nondiabetics. In stratified groups, the total SOD activity was different for the hypertensive diabetics compared to the prediabetics and normotensive controls. Lipid peroxidation was significantly higher in both groups of diabetics (hypertensive and normotensive) compared to prediabetic groups and hypertensive and normotensive controls. There was no significant difference in the CAT and GPx activities, as well as in the concentration of total thiols in the groups studied. Present data strongly suggest the involvement of oxidative stress in the pathophysiology of diabetes, revealing that the increased lipid peroxidation has a close relationship with high glucose levels, as observed by the fasting glucose and HbA1c levels. The results evidence the correlation between lipid peroxidation and DM, irrespective of the presence of hypertension.

scholarly journals Overexpression of ThSOS from Tamarix hispida improves salt tolerance

2019 ◽  
Author(s):  
Zhongyuan Liu ◽  
Qingjun Xie ◽  
Feifei Tang ◽  
Jing Wu ◽  
Wenfang Dong ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Salt Stress ◽  
Abiotic Stresses ◽  
Bioinformatics Analysis ◽  
Functional Characterization ◽  
Hormone Treatment ◽  
Gene Overexpression ◽  
Tamarix Hispida ◽  
Ros Scavenging

Abstract Highlights ● The functional characterization of ThSOS genes were investigated by bioinformatics analysis and molecular characterization. ● ThSOS genes can respond to abiotic stresses (salt and drought) and hormone treatment (ABA). ● ThSOS3 gene overexpression increased ROS-scavenging capability and decreasing lipid peroxidation in cell membrane. ● ThSOS3 could effectively enhance the tolerance of transgenic T. hispida and Arabidopsis to salt stress.

scholarly journals Properties of spermatozoal superoxide dismutase and lack of involvement of superoxides in metal-ion-catalysed lipid-peroxidation and reactions in semen

1980 ◽  
Vol 191 (2) ◽  
pp. 289-297 ◽  
Author(s):  
M R F Mennella ◽  
R Jones
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Metal Ion ◽  
Cold Shock ◽  
Total Activity ◽  
Cellular Damage ◽  
Purification And Characterization ◽  
Boar Spermatozoa

1. The distribution and properties of superoxide dismutase were examined in mammalian semen, and the enzyme was used to investigate the role of superoxides in metal-ion-catalysed lipid-peroxidation reactions in spermatozoa. 2. Superoxide dismutase activity was detected in seminal plasma and spermatozoa from all species studied, exceptionally high activity being found in donkey semen. The enzyme is easily solubilized from spermatozoa, as 85-90% of the total activity is released by cold shock, a relatively mild form of cellular damage. 3. Purification and characterization of the enzyme from supernatant fractions prepared from cold-shocked boar spermatozoa showed it to be cyanide-sensitive, to have a mol.wt. of 31 000, a pI of 5.9 and to contain 1.85 g-atoms of copper and 1.91 g-atoms of zinc per mol of protein. However, extensive sonication of spermatozoa released a small amount of a cyanide-insensitive enzyme, presumably a mangano superoxide dismutase, from the mitochondrial matrix. 4. The presence of superoxide dismutase in spermatozoa, either intracellularly or extracellularly, did not inhibit ascorbate/Fe2+-catalysed lipid-peroxidation reactions, suggesting that superoxides are not essential intermediates in this system.

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