scholarly journals Tuning macromolecular fate through site-selective chemical conjugation of a novel half-life extender

2020 ◽  
Author(s):  
Aimee Usera
Keyword(s):  
Half Life ◽  
Chemical Conjugation ◽  
Selective Chemical ◽  
Site Selective

scholarly journals Rational Generation of Lasso Peptides Based on Biosynthetic Gene Mutations and Site-Selective Chemical Modifications

2021 ◽  
Author(s):  
Tan Liu ◽  
Xiaojie Ma ◽  
Jiahui Yu ◽  
Wensheng Yang ◽  
guiyang wang ◽  
...  
Keyword(s):  
Natural Products ◽  
Gene Mutations ◽  
Proteolytic Degradation ◽  
Biosynthetic Gene ◽  
Chemical Modifications ◽  
Lasso Peptides ◽  
Selective Chemical ◽  
Site Selective

Lasso peptides are a unique family of natural products whose structures feature a specific threaded fold, which confers these peptides the resistance to thermal and proteolytic degradation. This stability gives...

scholarly journals Site-Selective Chemical State Analysis for Magnetite Structure Using Powder Spectro-Diffractometry

1999 ◽  
Vol 38 (S1) ◽  
pp. 381 ◽  
Author(s):  
Yanan Xiao ◽  
Shinjiro Hayakawa ◽  
Yohichi Gohshi ◽  
Masaharu Oshima ◽  
Hiroki Okudera ◽  
...  
Keyword(s):  
Chemical State ◽  
Selective Chemical ◽  
Site Selective ◽  
State Analysis

scholarly journals A Tale of Two Bioconjugations: pH Controlled Divergent Reactivity of Protein A-Oxo Aldehydes in Competing A-Oxo-Mannich and Catalyst-Free Aldol Ligations

2021 ◽  
Author(s):  
Tessa Keenan ◽  
Richard Spears ◽  
Saeed Akkad ◽  
Clare Mahon ◽  
Natasha E. Hatton ◽  
...  
Keyword(s):  
Chemical Biology ◽  
Protein A ◽  
Acidic Ph ◽  
Chemical Methods ◽  
New Methods ◽  
Catalyst Free ◽  
Selective Chemical ◽  
Site Selective ◽  
Novel Protein ◽  
Protein Enzyme

<div><div><div><p>Site-selective chemical methods for protein bioconjugation have revolutionised the fields of cell and chemical biology through the development of novel protein/enzyme probes bearing fluorescent, spectroscopic or even toxic cargos. Herein we report two new methods for the bioconjugation of a-oxo aldehyde handles within proteins using small molecule aniline and/or phenol probes. The ‘a-oxo-Mannich’ and ‘catalyst-free aldol’ ligations both compete for the electrophilic a-oxo aldehyde which displays pH divergent reactivity proceeding through the “Mannich” pathway at acidic pH to afford bifunctionalised bioconjugates, and the “catalyst-free aldol” pathway at neutral pH to afford monofunctionalised bioconjugates. We explore the substrate scope and utility of both these bioconjugations in the construction of neoglycoproteins, in the process formulating a mechanistic rationale for how both pathways intersect with each other at different reaction pH.</p></div></div></div>

nSite-Selective, Chemical Modification of Protein at Aromatic Side Chain and Their Emergent Applications

2021 ◽  
Vol 28 ◽  
Author(s):  
Arnab Chowdhury ◽  
Saurav Chatterjee ◽  
Akumlong Pongen ◽  
Dhanjit Sarania ◽  
Nitesh Mani Tripathi ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Chemical Reactivity ◽  
Future Perspective ◽  
Side Chain ◽  
Aromatic Side Chain ◽  
Therapeutic Applications ◽  
Selective Chemical ◽  
Photocatalytic Reactions ◽  
Metal Catalyzed ◽  
Site Selective

: Site-selective chemical modification of protein side chain has probed enormous opportunities in the fundamental understanding of cellular biology and therapeutic applications. Primarily, in the field of biopharmaceutical where formulation of bioconjugates is found to be potential medicine than an individual constituent. In this regard, Lysine and Cysteine are the most widely used endogenous amino acid for these purposes. Recently, the aromatic side chain residues (Trp, Tyr, and His) that are low abundant in protein have gained more attention in therapeutic applications due to their advantages of chemical reactivity and specificity. This review discusses the site-selective bioconjugation methods for aromatic side chains (Trp, Tyr and His) and highlights the developed strategies in the last three years, along with their applications. Also, the review highlights the prevalent methods published earlier. We have examined that metal-catalyzed and photocatalytic reactions are gaining more attention for bioconjugation, though their practical operation is under development. The review has been summarized with the future perspective of protein and peptide conjugations contemplating therapeutic applications and challenges.

Site-selective Chemical Modification of Chymotrypsin Using a Peptidyl Diphenyl 1-Amino-2-phenylethylphosphonate Derivative

2013 ◽  
Vol 42 (8) ◽  
pp. 860-862 ◽  
Author(s):  
Shin Ono ◽  
Junya Murai ◽  
Takahiko Nakai ◽  
Hirofumi Kuroda ◽  
Yoshikazu Horino ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Selective Chemical ◽  
Site Selective

Single-Site Labeling of Native Proteins Enabled by a Chemoselective and Site-Selective Chemical Technology

2018 ◽  
Vol 140 (44) ◽  
pp. 15114-15123 ◽  
Author(s):  
Srinivasa Rao Adusumalli ◽  
Dattatraya Gautam Rawale ◽  
Usha Singh ◽  
Prabhanshu Tripathi ◽  
Rajesh Paul ◽  
...  
Keyword(s):  
Chemical Technology ◽  
Single Site ◽  
Native Proteins ◽  
Selective Chemical ◽  
Site Selective
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