Two-Dimensional Differential Gel Electrophoresis of a Cell Line Derived from a Breast Cancer Micrometastasis Revealed a Stem/Progenitor Cell Protein Profile

2009 ◽  
Vol 8 (4) ◽  
pp. 2004-2014 ◽  
Author(s):  
Kai Bartkowiak ◽  
Marek Wieczorek ◽  
Friedrich Buck ◽  
Sönke Harder ◽  
Jennifer Moldenhauer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Gel Electrophoresis ◽  
Progenitor Cell ◽  
Protein Profile ◽  
Cell Protein ◽  
Two Dimensional ◽  
A Cell ◽  
Differential Gel Electrophoresis

Secretomic and Proteomic Analysis of Potential Breast Cancer Markers by Two-Dimensional Differential Gel Electrophoresis

2010 ◽  
Vol 9 (3) ◽  
pp. 1302-1322 ◽  
Author(s):  
Tzu-Chia Lai ◽  
Hsiu-Chuan Chou ◽  
Yi-Wen Chen ◽  
Tian-Ren Lee ◽  
Hsin-Tsu Chan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Cancer Markers ◽  
Differential Gel Electrophoresis

scholarly journals A Cell-Line-Specific Atlas of PARP-Mediated Protein Asp/Glu-ADP-Ribosylation in Breast Cancer

Cell Reports ◽  
2017 ◽  
Vol 21 (8) ◽  
pp. 2326-2337 ◽  
Author(s):  
Yuanli Zhen ◽  
Yajie Zhang ◽  
Yonghao Yu
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Adp Ribosylation ◽  
A Cell

scholarly journals Using 2-D electrophoresis followed by nano HPLC in nuclear protein analysis of MCF-7 breast cancer cell line by MALDI-TOF/TOF

2012 ◽  
Vol 10 (2) ◽  
pp. 407-412
Author(s):  
Soňa Tkáčiková ◽  
Ivan Talian ◽  
Marián Petrovič ◽  
Ján Sabo
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Gel Electrophoresis ◽  
Cancer Cell Line ◽  
Mass Analyzer ◽  
Maldi Tof ◽  
Mcf 7

AbstractThe biomarker identification is an important tool in early cancer detection. The MCF-7 breast cancer cell line was chosen as a model system. The nuclear proteins were extracted utilizing a commercially manufactured kit and separated on two dimensional (2-D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The first dimension was performed on isoelectric focusing strips with pH range 4–7. Afterwards the proteins were tryptic digested and identification was performed by matrix assisted laser desorption technique with time of flight mass analyzer (MALDI-TOF/TOF). For unambiguous identification proteins with too low concentration or spots contains protein mixture the nano high performance liquid chromatography (HPLC) was used. The 2-D gel electrophoresis (2-DGE) seems to be a good tool to separate large amount of proteins using relatively simple procedure and its hyphenation with HPLC can create the perfect analytical solution for proteome identification. About 150 nuclei protein spots were visualized and the most abundant of them were identified.

scholarly journals Proteomics Analysis by Two-Dimensional Differential Gel Electrophoresis Reveals the Lack of a Broad Response of Neisseria meningitidis to In Vitro-Produced AI-2

2005 ◽  
Vol 187 (1) ◽  
pp. 392-395 ◽  
Author(s):  
Stephan Schauder ◽  
Lucia Penna ◽  
Adeline Ritton ◽  
Catherine Manin ◽  
Fabienne Parker ◽  
...  
Keyword(s):  
Protein Expression ◽  
Neisseria Meningitidis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Differential Protein Expression ◽  
Proteomics Analysis ◽  
Global Response ◽  
Differential Gel Electrophoresis ◽  
Signaling Activity

ABSTRACT To investigate the effect of the autoinducer AI-2 on protein expression in Neisseria meningitidis, a luxS mutant of strain MC58 was grown in the presence and absence of in vitro-produced AI-2, and differential protein expression was assessed by two-dimensional differential gel electrophoresis. N. meningitidis did not show a global response to AI-2 signaling activity.

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