Wild-Type and Mutant Bovine Odorant-Binding Proteins To Probe the Role of the Quaternary Structure Organization in the Protein Thermal Stability

2008 ◽  
Vol 7 (12) ◽  
pp. 5221-5229 ◽  
Author(s):  
Anna Marabotti ◽  
Andrea Scirè ◽  
Maria Staiano ◽  
Roberta Crescenzo ◽  
Vincenzo Aurilia ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Binding Proteins ◽  
Quaternary Structure ◽  
Wild Type ◽  
Odorant Binding Proteins ◽  
Protein Thermal Stability ◽  
Odorant Binding

scholarly journals Antennal Enriched Odorant Binding Proteins Are Required for Odor Communication in Glossina f. fuscipes

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 541
Author(s):  
Souleymane Diallo ◽  
Mohd Shahbaaz ◽  
JohnMark O. Makwatta ◽  
Jackson M. Muema ◽  
Daniel Masiga ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Tissue Expression ◽  
Tsetse Fly ◽  
Tsetse Flies ◽  
Odorant Binding Proteins ◽  
Male Antenna ◽  
Female Antenna ◽  
Odor Communication ◽  
Odorant Binding

Olfaction is orchestrated at different stages and involves various proteins at each step. For example, odorant-binding proteins (OBPs) are soluble proteins found in sensillum lymph that might encounter odorants before reaching the odorant receptors. In tsetse flies, the function of OBPs in olfaction is less understood. Here, we investigated the role of OBPs in Glossina fuscipes fuscipes olfaction, the main vector of sleeping sickness, using multidisciplinary approaches. Our tissue expression study demonstrated that GffLush was conserved in legs and antenna in both sexes, whereas GffObp44 and GffObp69 were expressed in the legs but absent in the antenna. GffObp99 was absent in the female antenna but expressed in the male antenna. Short odorant exposure induced a fast alteration in the transcription of OBP genes. Furthermore, we successfully silenced a specific OBP expressed in the antenna via dsRNAi feeding to decipher its function. We found that silencing OBPs that interact with 1-octen-3-ol significantly abolished flies’ attraction to 1-octen-3-ol, a known attractant for tsetse fly. However, OBPs that demonstrated a weak interaction with 1-octen-3-ol did not affect the behavioral response, even though it was successfully silenced. Thus, OBPs’ selective interaction with ligands, their expression in the antenna and their significant impact on behavior when silenced demonstrated their direct involvement in olfaction.

scholarly journals Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Heliyon ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. e06427
Author(s):  
Haixia Zhan ◽  
Du Li ◽  
Youssef Dewer ◽  
Changying Niu ◽  
Fengqi Li ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Functional Characterization ◽  
Drosophila Suzukii ◽  
Odorant Binding Proteins ◽  
Odorant Binding

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

2019 ◽  
Vol 27 (5) ◽  
pp. 1019-1030 ◽  
Author(s):  
Ling Wang ◽  
Ying‐Dong Bi ◽  
Ming Liu ◽  
Wei Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Expression Profiles ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Pheromone Binding to General Odorant-binding Proteins from the Navel Orangeworm

2010 ◽  
Vol 36 (7) ◽  
pp. 787-794 ◽  
Author(s):  
Zhao Liu ◽  
Diogo M. Vidal ◽  
Zainulabeuddin Syed ◽  
Yuko Ishida ◽  
Walter S. Leal
Keyword(s):  
Binding Proteins ◽  
Navel Orangeworm ◽  
Odorant Binding Proteins ◽  
Odorant Binding
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