Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens , Sogatella furcifera , and Laodelphax striatellus

2019 ◽  
Vol 75 (6) ◽  
pp. 1606-1620 ◽  
Author(s):  
Peng He ◽  
Guang‐Lei Chen ◽  
Shuo Li ◽  
Jun Wang ◽  
Yun‐Feng Ma ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Binding Proteins ◽  
Nilaparvata Lugens ◽  
Laodelphax Striatellus ◽  
Sogatella Furcifera ◽  
Odorant Binding Proteins ◽  
Rice Planthoppers ◽  
Odorant Binding

scholarly journals Distinct Expression Profiles and Different Functions of Odorant Binding Proteins in Nilaparvata lugens Stål

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28921 ◽  
Author(s):  
Peng He ◽  
Jin Zhang ◽  
Nai-Yong Liu ◽  
Ya-Nan Zhang ◽  
Ke Yang ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Expression Profiles ◽  
Nilaparvata Lugens ◽  
Odorant Binding Proteins ◽  
Nilaparvata Lugens Stål ◽  
Odorant Binding

scholarly journals Three odorant-binding proteins are involved in the behavioral response of Sogatella furcifera to rice plant volatiles

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6576 ◽  
Author(s):  
Kui Hu ◽  
Sheng Liu ◽  
Lin Qiu ◽  
Youzhi Li
Keyword(s):  
Rice Plant ◽  
Plant Volatiles ◽  
Binding Proteins ◽  
Signal Transduction Pathway ◽  
Insect Behavior ◽  
Binding Affinities ◽  
Sogatella Furcifera ◽  
Odorant Binding Proteins ◽  
Weak Binding ◽  
Odorant Binding

Plant volatiles play an important role in regulating insect behavior. Odorant binding proteins (OBPs) are involved in the first step of the olfactory signal transduction pathway and plant volatiles recognition. Sogatella furcifera is one of the most destructive pests of rice crops. Understanding the functions of S. furcifera OBPs (SfurOBPs) in the host plant location and the behavioral responses of S. furcifera to rice plant volatiles could lead to improved, more environmentally-friendly, methods for controlling this pest. We found that SfurOBP1 displayed only weak binding with all the tested volatiles. SfurOBP2, SfurOBP3 and SfurOBP11 had different binding affinities to β-ionone. SfurOBP2 and SfurOBP11 had strong binding affinities to β-caryophyllene (Ki = 2.23 µM) and plant alcohol (Ki = 2.98 µM), respectively. The results of Y-olfactometer experiments indicate that S. furcifera was significantly repelled by octanal and n-octane but strongly attracted by (+)-limonene, acetophenone, 2-heptanone, n-hendecane, α-farnesene and β-ionone. Furthermore, the dsRNA-mediated gene silencing of SfurOBP2, SfurOBP3 and SfurOBP11 shifted the olfactory behavior of S. furcifera for β-ionone, α-farnesene and plant alcohol, respectively. These results suggest that the SfurOBPs are involved in the recognition of rice plant volatiles, and several potential repellants and lures for controlling this pest.

scholarly journals Molecular Characterization of Ca2+/Calmodulin-Dependent Protein Kinase II Isoforms in Three Rice Planthoppers—Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera

2019 ◽  
Vol 20 (12) ◽  
pp. 3014
Author(s):  
Wei-Xia Wang ◽  
Feng-Xiang Lai ◽  
Pin-Jun Wan ◽  
Qiang Fu ◽  
Ting-Heng Zhu
Keyword(s):  
Amino Acid ◽  
Protein Kinase ◽  
Variable Region ◽  
Nilaparvata Lugens ◽  
Laodelphax Striatellus ◽  
Sogatella Furcifera ◽  
Dependent Protein Kinase ◽  
Rice Planthoppers ◽  
Protein Kinase Ii ◽  
Dependent Protein

This study reports the identification of splice variants for the calcium/calmodulin-dependent protein kinase II (CaMKII) gene from Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera. CaMKII is a multifunctional serine/threonine protein kinase that transduces Ca2+ signals in cells to control a range of cellular processes in the nervous system and muscular tissue. Sequence analysis showed that CaMKII was 99.0% identical at the amino acid level among three rice planthoppers, with the exception of a variable region located in the association domain. Four kinds of 20–81 amino acid “inserts” were found in the variable region. The phylogenetic tree of the deduced amino acid sequences showed that the NlCaMKII isoforms were more closely related to the LsCaMKII isoforms and were slightly distinct from SfCaMKII. CaMKII-E was the dominant type among the five main isoforms. CaMKII genes were constitutively expressed in various nymphal and adult stages and in tested tissues with the predominant transcription occurring in the head. There was no major tissue specificity of isoform expression, but the expression pattern and relative abundance of isoforms varied when compared with the RT-PCR between tissues. In addition, RNAi in N. lugens with dsRNA at a concentration of 200 ng nymph−1 induced a mortality of 77.7% on the 10th day and a reduction in the mRNA expression level of 67.2%. Unlike the holometabolous insect Helicoverpa armigera, the knockdown of NlCaMKII did not suppress the expression of 20E response genes, such as ECR, USP1, and HR3, in N. lugens. These results indicate that the role of CaMKII in hemimetabolous insects may be different from that in holometabolous insects.

scholarly journals Comparison of upper sublethal and lethal temperatures in three species of rice planthoppers

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shahbaz Ali ◽  
Pei Li ◽  
Asad Ali ◽  
Maolin Hou
Keyword(s):  
Plant Material ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Laodelphax Striatellus ◽  
Sogatella Furcifera ◽  
Lethal Temperatures ◽  
Rice Planthoppers ◽  
Critical Thermal Maxima ◽  
Increasing Temperature ◽  
Occurrence Patterns

Abstract Temperature is an important environmental factor for ectotherms’ fitness and survival. The upper sublethal and lethal temperatures were compared between adults of three closely related destructive planthopper species, the small brown planthopper (Laodelphax striatellus, SBPH), the brown planthopper (Nilaparvata lugens, BPH), and the white-backed planthopper (Sogatella furcifera, WBPH) in the absence and presence of the host plant (Oryza sativa, var. Taichong1). Values of the critical thermal maxima (CTmax) were higher in SBPH than in both BPH and WBPH and higher in BPH than in WBPH, and values of the heat coma temperatures (HCT) were higher in both BPH and SBPH than in WBPH. CTmax and HCT values were higher in the presence than in the absence of plant material. Between sexes, females generally showed higher CTmax and HCT than males. The upper lethal temperatures (ULT50) measured in the absence of plant material were not significantly different among the planthopper species. The planthoppers also exhibited different behaviors in an increasing temperature regime, with fewer insects dropping-off from the plant in SBPH than in BPH and WBPH. These results indicate that SBPH and BPH are more heat tolerant than WBPH. The findings highlight the biological divergence in closely related planthopper species and the importance of performing the heat tolerance measurement in an ecologically relevant setting, which serves to predict seasonal and spatial occurrence patterns of the destructive planthopper species.

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