Influence of the Protein Staining in the Fast Ultrasonic Sample Treatment for Protein Identification through Peptide Mass Fingerprint and Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

2008 ◽  
Vol 7 (5) ◽  
pp. 2097-2106 ◽  
Author(s):  
M. Galesio ◽  
D. V. Vieira ◽  
R. Rial-Otero ◽  
C. Lodeiro ◽  
I. Moura ◽  
...  
Keyword(s):  
Protein Identification ◽  
Laser Desorption ◽  
Sample Treatment ◽  
Peptide Mass Fingerprint ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Protein Staining ◽  
Matrix Assisted Laser

scholarly journals Proteomic analysis of different extracts from barley grains

2011 ◽  
Vol 48 (No. 6) ◽  
pp. 261-264 ◽  
Author(s):  
J. Chmelík ◽  
P. Řehulka ◽  
M. Střelcová ◽  
V. Kubáň ◽  
C. Mayrhofer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Protein Identification ◽  
Laser Desorption ◽  
Good Sensitivity ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Barley Grains ◽  
Matrix Assisted Laser

Identification of several proteins extracted with various solvents from barley grains was performed by the proteomic strategy based on a combination of gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and bioinformatics. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. In order to simplify the protein mixture extracted from grains, we used sequential extractions of grains with various solvents (based on modified Osborne system). Several proteins (e.g. β-amylase, B3-hordein) were identified in this way. It seems that this approach can become an important tool for proteomics of cereals.

scholarly journals Identification of Polypeptides Expressed in Response to Vinyl Chloride, Ethene, and Epoxyethane in Nocardioides sp. Strain JS614 by Using Peptide Mass Fingerprinting

2007 ◽  
Vol 73 (13) ◽  
pp. 4368-4372 ◽  
Author(s):  
Adina S. Chuang ◽  
Timothy E. Mattes
Keyword(s):  
Vinyl Chloride ◽  
Laser Desorption ◽  
Peptide Mass Fingerprinting ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.

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