Simplifying sample handling for protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

2007 ◽  
Vol 21 (20) ◽  
pp. 3269-3278 ◽  
Author(s):  
Francisco M. Cordeiro ◽  
Ricardo J. Carreira ◽  
Raquel Rial-Otero ◽  
M. Gabriela Rivas ◽  
Isabel Moura ◽  
...  
Keyword(s):  
Protein Identification ◽  
Laser Desorption ◽  
Peptide Mass Fingerprint ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Sample Handling ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Identification of Polypeptides Expressed in Response to Vinyl Chloride, Ethene, and Epoxyethane in Nocardioides sp. Strain JS614 by Using Peptide Mass Fingerprinting

2007 ◽  
Vol 73 (13) ◽  
pp. 4368-4372 ◽  
Author(s):  
Adina S. Chuang ◽  
Timothy E. Mattes
Keyword(s):  
Vinyl Chloride ◽  
Laser Desorption ◽  
Peptide Mass Fingerprinting ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.

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