Label-free Selected Reaction Monitoring Enables Multiplexed Quantitation of S100 Protein Isoforms in Cancer Cells

2013 ◽  
Vol 12 (8) ◽  
pp. 3679-3688 ◽  
Author(s):  
Juan Martínez-Aguilar ◽  
Mark P. Molloy
Keyword(s):  
Cancer Cells ◽  
S100 Protein ◽  
Protein Isoforms ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Label Free

scholarly journals Constrained selected reaction monitoring: Quantification of selected post-translational modifications and protein isoforms

Methods ◽  
2013 ◽  
Vol 61 (3) ◽  
pp. 304-312 ◽  
Author(s):  
Xiaoqian Liu ◽  
Zhicheng Jin ◽  
Richard O’Brien ◽  
Joan Bathon ◽  
Harry C. Dietz ◽  
...  
Keyword(s):  
Protein Isoforms ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Post Translational Modifications

scholarly journals A general strategy for studying multisite protein phosphorylation using label-free selected reaction monitoring mass spectrometry

2011 ◽  
Vol 418 (2) ◽  
pp. 267-275 ◽  
Author(s):  
Christie L. Eissler ◽  
Steven C. Bremmer ◽  
Juan S. Martinez ◽  
Laurie L. Parker ◽  
Harry Charbonneau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Phosphorylation ◽  
Selected Reaction Monitoring ◽  
General Strategy ◽  
Reaction Monitoring ◽  
Label Free

scholarly journals Automated Selected Reaction Monitoring Software for Accurate Label-Free Protein Quantification

2012 ◽  
Vol 11 (7) ◽  
pp. 3766-3773 ◽  
Author(s):  
Johan Teleman ◽  
Christofer Karlsson ◽  
Sofia Waldemarson ◽  
Karin Hansson ◽  
Peter James ◽  
...  
Keyword(s):  
Protein Quantification ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Label Free ◽  
Free Protein ◽  
Monitoring Software

scholarly journals Quantitative Analysis of Energy Metabolic Pathways in MCF-7 Breast Cancer Cells by Selected Reaction Monitoring Assay

2012 ◽  
Vol 11 (8) ◽  
pp. 422-434 ◽  
Author(s):  
Andrei P. Drabovich ◽  
Maria P. Pavlou ◽  
Apostolos Dimitromanolakis ◽  
Eleftherios P. Diamandis
Keyword(s):  
Breast Cancer ◽  
Quantitative Analysis ◽  
Cancer Cells ◽  
Metabolic Pathways ◽  
Breast Cancer Cells ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Mcf 7

scholarly journals Label-Free Quantitation of Protein Modifications by Pseudo Selected Reaction Monitoring with Internal Reference Peptides

2012 ◽  
Vol 11 (6) ◽  
pp. 3467-3479 ◽  
Author(s):  
Stacy D. Sherrod ◽  
Matthew V. Myers ◽  
Ming Li ◽  
Jeremy S. Myers ◽  
Kristin L. Carpenter ◽  
...  
Keyword(s):  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Label Free ◽  
Internal Reference ◽  
Protein Modifications ◽  
Label Free Quantitation

scholarly journals Discovery of a human testis-specific protein complex TEX101-DPEP3 and selection of its disrupting antibodies

2018 ◽  
Author(s):  
Christina Schiza ◽  
Dimitrios Korbakis ◽  
Efstratia Panteleli ◽  
Keith Jarvi ◽  
Andrei P. Drabovich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Surface ◽  
Protein Interactions ◽  
Specific Protein ◽  
Selected Reaction Monitoring ◽  
Human Testis ◽  
Specific Cell ◽  
Reaction Monitoring ◽  
Label Free ◽  
Affinity Capture

SUMMARYTEX101 is a testis-specific protein expressed exclusively in male germ cells and is a validated biomarker of male infertility. Studies in mice suggest that TEX101 is a cell-surface chaperone which regulates, through protein-protein interactions, the maturation of proteins involved in spermatozoa transit and oocyte binding. Male TEX101-null mice are sterile. Here, we identified by co-immunoprecipitation-mass spectrometry the interactome of human TEX101 in testicular tissues and spermatozoa. The testis-specific cell-surface dipeptidase 3 (DPEP3) emerged as the top hit. We further validated the TEX101-DPEP3 complex by using hybrid immunoassays. Combinations of antibodies recognizing different epitopes of TEX101 and DPEP3 facilitated development of a simple immunoassay to screen for disruptors of TEX101-DPEP3 complex. As a proof-of-a-concept, we demonstrated that anti-TEX101 antibody T4 disrupted the native TEX101-DPEP3 complex. Disrupting antibodies may be used to study the human TEX101-DPEP3 complex, and to develop modulators for male fertility.Non-standard abbreviationsTEX101Testis-expressed sequence 101 proteinDPEP3Dipeptidase 3AC-MSAffinity capture-mass spectrometryco-IP-MSCoimmunoprecipitation-mass spectrometryGPIGlycosylphosphatidylinositolLFQLabel-free quantificationmAbMonoclonal antibodyNHSN-hydroxysuccinimidePRMParallel reaction monitoringPTMPosttranslational modificationSPSeminal plasmaSRMSelected reaction monitoringFDRfalse detection rate

Use of selected reaction monitoring data for label-free quantification of protein modification stoichiometry

PROTEOMICS ◽  
2010 ◽  
Vol 10 (23) ◽  
pp. 4301-4305 ◽  
Author(s):  
Deepa Balasubramaniam ◽  
Christie L. Eissler ◽  
Cynthia V. Stauffacher ◽  
Mark C. Hall
Keyword(s):  
Protein Modification ◽  
Monitoring Data ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsia

2013 ◽  
Vol 12 (11) ◽  
pp. 3148-3159 ◽  
Author(s):  
Richard T. Blankley ◽  
Christal Fisher ◽  
Melissa Westwood ◽  
Robyn North ◽  
Philip N. Baker ◽  
...  
Keyword(s):  
Early Onset ◽  
Predictive Markers ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Label Free
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