scholarly journals A general strategy for studying multisite protein phosphorylation using label-free selected reaction monitoring mass spectrometry

2011 ◽  
Vol 418 (2) ◽  
pp. 267-275 ◽  
Author(s):  
Christie L. Eissler ◽  
Steven C. Bremmer ◽  
Juan S. Martinez ◽  
Laurie L. Parker ◽  
Harry Charbonneau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Phosphorylation ◽  
Selected Reaction Monitoring ◽  
General Strategy ◽  
Reaction Monitoring ◽  
Label Free

scholarly journals Measurement of Protein Phosphorylation Stoichiometry by Selected Reaction Monitoring Mass Spectrometry

2010 ◽  
Vol 9 (5) ◽  
pp. 2752-2761 ◽  
Author(s):  
Lily L. Jin ◽  
Jiefei Tong ◽  
Amol Prakash ◽  
Scott M. Peterman ◽  
Jonathan R. St-Germain ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Phosphorylation ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring

scholarly journals Discovery of a human testis-specific protein complex TEX101-DPEP3 and selection of its disrupting antibodies

2018 ◽  
Author(s):  
Christina Schiza ◽  
Dimitrios Korbakis ◽  
Efstratia Panteleli ◽  
Keith Jarvi ◽  
Andrei P. Drabovich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Surface ◽  
Protein Interactions ◽  
Specific Protein ◽  
Selected Reaction Monitoring ◽  
Human Testis ◽  
Specific Cell ◽  
Reaction Monitoring ◽  
Label Free ◽  
Affinity Capture

SUMMARYTEX101 is a testis-specific protein expressed exclusively in male germ cells and is a validated biomarker of male infertility. Studies in mice suggest that TEX101 is a cell-surface chaperone which regulates, through protein-protein interactions, the maturation of proteins involved in spermatozoa transit and oocyte binding. Male TEX101-null mice are sterile. Here, we identified by co-immunoprecipitation-mass spectrometry the interactome of human TEX101 in testicular tissues and spermatozoa. The testis-specific cell-surface dipeptidase 3 (DPEP3) emerged as the top hit. We further validated the TEX101-DPEP3 complex by using hybrid immunoassays. Combinations of antibodies recognizing different epitopes of TEX101 and DPEP3 facilitated development of a simple immunoassay to screen for disruptors of TEX101-DPEP3 complex. As a proof-of-a-concept, we demonstrated that anti-TEX101 antibody T4 disrupted the native TEX101-DPEP3 complex. Disrupting antibodies may be used to study the human TEX101-DPEP3 complex, and to develop modulators for male fertility.Non-standard abbreviationsTEX101Testis-expressed sequence 101 proteinDPEP3Dipeptidase 3AC-MSAffinity capture-mass spectrometryco-IP-MSCoimmunoprecipitation-mass spectrometryGPIGlycosylphosphatidylinositolLFQLabel-free quantificationmAbMonoclonal antibodyNHSN-hydroxysuccinimidePRMParallel reaction monitoringPTMPosttranslational modificationSPSeminal plasmaSRMSelected reaction monitoringFDRfalse detection rate

scholarly journals Selected reaction monitoring mass spectrometry of mastitis milk reveals pathogen-specific regulation of bovine host response proteins

2018 ◽  
Vol 101 (7) ◽  
pp. 6532-6541 ◽  
Author(s):  
Ulrike Kusebauch ◽  
Lorenzo E. Hernández-Castellano ◽  
Stine L. Bislev ◽  
Robert L. Moritz ◽  
Christine M. Røntved ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Response ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Specific Regulation
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