Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek’s Disease Virus

2012 ◽  
Vol 11 (12) ◽  
pp. 5663-5677 ◽  
Author(s):  
Ko-yi Chien ◽  
Kevin Blackburn ◽  
Hsiao-Ching Liu ◽  
Michael B. Goshe
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

scholarly journals Induction of Host Gene Expression following Infection of Chicken Embryo Fibroblasts with Oncogenic Marek's Disease Virus

2001 ◽  
Vol 75 (1) ◽  
pp. 533-539 ◽  
Author(s):  
Robin W. Morgan ◽  
Luc Sofer ◽  
Amy S. Anderson ◽  
Erin L. Bernberg ◽  
Jing Cui ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Class I Mhc ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

ABSTRACT Microarrays containing 1,126 nonredundant cDNAs selected from a chicken activated T-cell expressed sequence tag database (http://chickest.udel.edu ) were used to examine changes in host cell gene expression that accompany infection of chicken embryo fibroblasts (CEF) with Marek's disease virus (MDV). Host genes that were reproducibly induced by infection of CEF with the oncogenic RB1B strain of MDV included macrophage inflammatory protein, interferon response factor 1, interferon-inducible protein, quiescence-specific protein, thymic shared antigen 1, major histocompatibility complex (MHC) class I, MHC class II, β2-microglobulin, clusterin, interleukin-13 receptor alpha chain, ovotransferrin, a serine/threonine kinase, and avian leukosis virus subgroup J glycoprotein.

Ovotransferrin expression and release by chicken cell lines infected with Marek’s disease virus

2007 ◽  
Vol 85 (1) ◽  
pp. 150-155 ◽  
Author(s):  
Francesco Giansanti ◽  
M. Federica Giardi ◽  
M. Teresa Massucci ◽  
Dario Botti ◽  
Giovanni Antonini
Keyword(s):  
Antiviral Activity ◽  
Viral Infection ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Lymphoblastoid Cells ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Mammals posses both serum transferrin and lactoferrin, whose functions are taken over in birds by ovotransferrin, displaying both iron transport and antibacterial activities. Ovotransferrin also exerts antiviral activity towards Marek’s disease virus, an avian member of the herpes family of viruses. This virus infects lymphoid organs and induces the transcription of ovotransferrin in infected chicken embryo fibroblasts. However, it has not yet been established whether ovotransferrin gene transcription is linked to the release of the protein outside the cells or whether ovotransferrin expression and release also occurs in chicken lymphoblastoid cells in which the Marek’s disease viral genome is integrated. Our results indicate that both serum and egg-white isoforms of ovotransferrin are expressed and released in the supernatants of chicken embryo fibroblast and lymphoblastoid cells in the absence of infection. Viral infection of chicken embryo fibroblasts caused a slight increase of ovotransferrin release, whereas viral reinfection of lymphoblastoid cells caused a remarkable ovotransferrin release in a virus concentration-dependent manner. These findings suggest that ovotransferrin release in vivo may play a crucial role in protecting the whole organism from viral infection spreading, and support the hypothesis that the antiviral activity of ovotransferrin is an important part of the innate immune response in birds, resembling the antiviral activity of lactoferrin in mammals.

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