scholarly journals Proteome of Human Subcutaneous Adipose Tissue Stromal Vascular Fraction Cells versus Mature Adipocytes Based on DIGE

2011 ◽  
Vol 10 (4) ◽  
pp. 1519-1527 ◽  
Author(s):  
Indu Kheterpal ◽  
Ginger Ku ◽  
Liana Coleman ◽  
Gang Yu ◽  
Andrey A. Ptitsyn ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Subcutaneous Adipose

scholarly journals Comparison of infrapatellar and subcutaneous adipose tissue stromal vascular fraction and stromal/stem cells in osteoarthritic subjects

2012 ◽  
Vol 8 (10) ◽  
pp. 757-762 ◽  
Author(s):  
Pedro Pires de Carvalho ◽  
Katie M. Hamel ◽  
Robert Duarte ◽  
Andrew G. S. King ◽  
Masudul Haque ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Subcutaneous Adipose ◽  
Stromal Stem Cells

scholarly journals A novel conjunctive microenvironment derived from human subcutaneous adipose tissue contributes to physiology of its superficial layer

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Leandra Santos Baptista ◽  
Isis Côrtes ◽  
Bianca Montenegro ◽  
Cesar Claudio-da-Silva ◽  
Marielle Bouschbacher ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Ex Vivo ◽  
Binding Protein ◽  
Receptor Gene ◽  
Stromal Vascular Fraction ◽  
Superficial Fascia ◽  
Fatty Acid Binding ◽  
Retinacula Cutis ◽  
Subcutaneous Adipose

Abstract Background In human subcutaneous adipose tissue, the superficial fascia distinguishes superficial and deep microenvironments showing extensions called retinacula cutis. The superficial subcutaneous adipose tissue has been described as hyperplastic and the deep subcutaneous adipose tissue as inflammatory. However, few studies have described stromal-vascular fraction (SVF) content and adipose-derived stromal/stem cells (ASCs) behavior derived from superficial and deep subcutaneous adipose tissue. In this study, we analyzed a third conjunctive microenvironment: the retinacula cutis superficialis derived from superficial subcutaneous adipose tissue. Methods The samples of abdominal human subcutaneous adipose tissue were obtained during plastic aesthetic surgery in France (Declaration DC-2008-162) and Brazil (Protocol 145/09). Results The SVF content was characterized in situ by immunofluorescence and ex vivo by flow cytometry revealing a high content of pre-adipocytes rather in superficial subcutaneous adipose tissue microenvironment. Adipogenic assays revealed higher percentage of lipid accumulation area in ASCs from superficial subcutaneous adipose tissue compared with retinacula cutis superficialis (p < 0.0001) and deep subcutaneous adipose tissue (p < 0.0001). The high adipogenic potential of superficial subcutaneous adipose tissue was corroborated by an up-regulation of adipocyte fatty acid-binding protein (FABP4) compared with retinacula cutis superficialis (p < 0.0001) and deep subcutaneous adipose tissue (p < 0.0001) and of C/EBPα (CCAAT/enhancer-binding protein alpha) compared with retinacula cutis superficialis (p < 0.0001) and deep subcutaneous adipose tissue (p < 0.0001) microenvironments. Curiously, ASCs from retinacula cutis superficialis showed a higher level of adiponectin receptor gene compared with superficial subcutaneous adipose tissue (p = 0.0409), widely known as an anti-inflammatory hormone. Non-induced ASCs from retinacula cutis superficialis showed higher secretion of human vascular endothelial growth factor (VEGF), compared with superficial (p = 0.0485) and deep (p = 0.0112) subcutaneous adipose tissue and with adipogenic-induced ASCs from superficial (p = 0.0175) and deep (p = 0.0328) subcutaneous adipose tissue. Furthermore, ASCs from retinacula cutis superficialis showed higher secretion of Chemokine (C–C motif) ligand 5 (CCL5) compared with non-induced (p = 0.0029) and induced (p = 0.0089) superficial subcutaneous adipose tissue. Conclusions This study highlights the contribution to ASCs from retinacula cutis superficialis in their angiogenic property previously described for the whole superficial subcutaneous adipose tissue besides supporting its adipogenic potential for superficial subcutaneous adipose tissue.

scholarly journals Macrophage Phenotypes in the Adipose Tissue of Postmenopausal Women

2015 ◽  
pp. S427-S433
Author(s):  
A. KRÁLOVÁ ◽  
I. KRÁLOVÁ LESNÁ ◽  
J. FRONĚK ◽  
S. ČEJKOVÁ ◽  
A. SEKERKOVÁ ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Postmenopausal Women ◽  
Subcutaneous Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Premenopausal Women ◽  
Kidney Donors ◽  
Positive Correlation ◽  
Living Kidney Donors ◽  
Membrane Bound ◽  
Subcutaneous Adipose

Atherosclerosis pathology is the interplay between high intravascular LDL particle concentration and monocyte/macrophage presence within the sub-endothelial space of the artery. In this project, phenotypes of macrophages connected with subclinical inflammation in adipose tissue of living kidney donors were studied. Samples of subcutaneous adipose tissue of living kidney donors (n=36) were exposed to collagenase. Stromal vascular fraction (SVF) was eluted from the samples, then labeled with monoclonal antibodies (anti-CD14 and anti-calprotectin), conjugated with fluorochromes and analyzed by flow cytometry. The positive correlation between the number of total macrophages and calprotectin-positive macrophages with BMI in the subcutaneous adipose tissue of postmenopausal women was demonstrated (p<0.05; R=0.43 and p<0.01; R=0.60), whereas no positive correlation in premenopausal women and men was shown. In conclusion, we documented a significant effect of BMI increase on the presence of total macrophages in adipose tissue of postmenopausal women, in contrast to premenopausal women. This difference was much more pronounced when proinflammatory macrophages with membrane-bound calprotectin were analyzed.

Insulin resistance, systemic and regional inflammation in subcutaneous adipose tissue during weight loss in a population with obesity

2019 ◽  
Author(s):  
Frederique Van de Velde ◽  
Margriet Ouwens ◽  
Arsene-Helene Batens ◽  
Samyah Shadid ◽  
Bruno Lapauw ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Weight Loss ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose

701-P: Effects of Exercise on Extracellular Matrix Modifiers in Subcutaneous Adipose Tissue of Obese Adults

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 701-P
Author(s):  
PALLAVI VARSHNEY ◽  
BENJAMIN J. RYAN ◽  
CHIWOON AHN ◽  
MICHAEL W. SCHLEH ◽  
JEFFREY F. HOROWITZ
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Obese Adults ◽  
Subcutaneous Adipose
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